BLAST
XX - BLAST results.ipynb
A
, B
, C
, or D
(check your post-its)mygroup
to the letter of your groupBLAST
search in the cellBLAST
outputQuery= query protein sequence
Length=400
Score
Sequences producing significant alignments: (Bits)
PITG_08491T0 Phytophthora infestans T30-4 choline transporter-l... 34.3
> PITG_08491T0 Phytophthora infestans T30-4 choline transporter-like
protein (441 aa)
Length=486
Score = 34.3 bits (77), Method: Compositional matrix adjust.
Identities = 22/69 (32%), Positives = 38/69 (55%), Gaps = 4/69 (6%)
Query 106 EVILPMMYQFALKPSFADVINDYKPYSKHTAGVSDQELKGEATTWMLADKNSRMKAFLSQ 165
E+++PM+Y L F ++ Y P HTA ++ EL+G T ++A+ S + F ++
Sbjct 40 ELMVPMLYSLYLVVLFHLPVSAYYP---HTASMTAHELQGAVITILVAETPSIIIQF-AK 95
Query 166 IKTKSNSSE 174
T SN S+
Sbjct 96 CHTSSNISQ 104
Query= query protein sequence
Length=400
Score
Sequences producing significant alignments: (Bits)
PITG_08491T0 Phytophthora infestans T30-4 choline transporter-l... 34.3
> PITG_08491T0 Phytophthora infestans T30-4 choline transporter-like
protein (441 aa)
Length=486
Score = 34.3 bits (77), Method: Compositional matrix adjust.
Identities = 22/69 (32%), Positives = 38/69 (55%), Gaps = 4/69 (6%)
Query 106 EVILPMMYQFALKPSFADVINDYKPYSKHTAGVSDQELKGEATTWMLADKNSRMKAFLSQ 165
E+++PM+Y L F ++ Y P HTA ++ EL+G T ++A+ S + F ++
Sbjct 40 ELMVPMLYSLYLVVLFHLPVSAYYP---HTASMTAHELQGAVITILVAETPSIIIQF-AK 95
Query 166 IKTKSNSSE 174
T SN S+
Sbjct 96 CHTSSNISQ 104
A
: ?B
: ?C
: ?D
: ?A
: 1e-05B
: 0.001C
: 0.1D
: 10BLAST
interpretationPITG\_?????T0
) are correct
dbA
contains only three different sequences: two are repeated 50,000 times (composition)query.fasta
is random sequence, not real protein (GIGO!)
nr
or PFam
matches