04 - Using NCBI BLAST+ Service with Biopython¶
Learning Outcomes¶
- Use of
NCBIremoteBLAST+using Python and Biopython in the Jupyter notebook - Creating a remote
BLAST+query, and its connection to running aBLAST+search on the server - Reading
BLAST+output from theNCBIserver into a Python variable - Using Entrez queries to customise your remote search
- Computational analysis and visualisation of
BLAST+output
Introduction¶
This notebook presents examples of methods for using `BLAST` programmatically, with the webservice provided by NCBI. All calculations will be run on NCBI's servers, using NCBI's databases (not your local `BLAST` installation), but you will be controlling the search using Python code in this notebook.
The advantages to using a programmatic interface for remote BLAST queries are the same as those listed in notebook 03:
- It is easy to set up repeatable searches for many sequences, or collections of sequences
- It is easy to read in the search results and conduct downstream analyses that add value to your search
Where it is impractical to submit a large number of similar queries via a web form (because it is tiresome to point-and-click over and over again), this can be handled programmatically instead. You have the opportunity to change custom options to help refine your query, just as in the website interface. If you need to repeat a query, it can be trivial to get the same settings every time, if you use a programmatic approach.
Python imports¶
To interact with the NCBI's `BLAST` service, we will again use the free `Biopython` programming tools. These provide an interface to interact with NCBI's `BLAST` server, run jobs, and to retrieve the output files.
We import these tools, and some standard library packages for working with files (os) below.
In [1]:
# Show plots as part of the notebook
%matplotlib inline
# Standard library packages
import os
# Import Biopython tools for running remote BLAST searches
from Bio.Blast import NCBIWWW
# Import Biopython SeqIO module to handle reading sequence data
from Bio import SeqIO
Running and analysing a remote BLASTX search¶
As in [notebook 03](03-programming_for_blast.ipynb), your first worked example will be to run a `BLASTX` search, querying a nucleotide sequence against a protein database, to identify potential homologues. What is different about this search is that you will be conducting it at NCBI, and using a different database.
You will use Biopython in the code blocks below to first perform the same BLASTX search you carried out in the exercise from notebook 01 - this will query a lantibiotic biosynthesis protein from Kitasatospora against other Kitasatospora species - and write the results to file. Then you will parse the results into a pandas dataframe, and finally plot some summary statistics using seaborn.
Run BLASTX¶
Running a remote `BLAST` search with `Biopython` is, in some ways, simpler than running a local `BLAST` query. The key steps are:
- Read the query sequence(s) from a source (possibly a local file, but maybe a remote database)
- Run a remote job with the
NCBIWWW.qblast()method, specifying your query sequence, database, andBLASTprogram - Parse the output you get back from NCBI
To run the remote job, you need the same kind of information as if you were running BLAST via the web interface - these arguments are compulsory:
- the
BLASTprogram to use - query sequence(s) to search with
- the database to search in
but you can provide some extra choices when you run the remote job, including restricting the remote search on the basis of taxonomy, just as you did in the exercise from notebook 01.
Firstly, you will obtain a query sequence for the search.
You will use the same penicillin-binding protein from notebook 01, reading it as before from a local file, but this time using Biopython's SeqIO() module.
When data such as biological sequences are read in, their metadata - information on database IDs, and other features - follows them. `Biopython` does a nice job of showing us this information if we look at it with the `print()` function:
In [2]:
# Define path to data directory
datadir = os.path.join("data", "kitasatospora")
# Load sequence of penicillin-binding protein, and inspect the information
seqfile = os.path.join(datadir, "k_sp_CB01950_penicillin.fasta")
seq = SeqIO.read(seqfile, "fasta")
print(seq)
ID: lcl|LISX01000001.1_cds_OKJ16671.1_31
Name: lcl|LISX01000001.1_cds_OKJ16671.1_31
Description: lcl|LISX01000001.1_cds_OKJ16671.1_31 [locus_tag=AMK19_00175] [protein=penicillin-binding protein] [protein_id=OKJ16671.1] [location=39730..41184]
Number of features: 0
Seq('GTGAACAAGCCGATCCGCCGGGTGTCGATCTTCTGCCTGGTCCTGATCCTGGCC...TAG', SingleLetterAlphabet())
However, the remote `BLAST` server requires us to present our sequence in `FASTA` format!
One of the clever things about Biopython's sequence objects - and a big advantage of using programmatic approaches - is that we can readily convert our sequence information into a number of different formats. To do this, we can use the sequence's .format() method to produce a FASTA-formatted string.
Doing this does not change the original sequence or its information in any way, but it creates a new presentation of that data, which we can use as our query:
In [3]:
# We need the sequence as a string, so use the .format() method
print(seq.format("fasta"))
>lcl|LISX01000001.1_cds_OKJ16671.1_31 [locus_tag=AMK19_00175] [protein=penicillin-binding protein] [protein_id=OKJ16671.1] [location=39730..41184] GTGAACAAGCCGATCCGCCGGGTGTCGATCTTCTGCCTGGTCCTGATCCTGGCCCTGATG CTCCGGGTGAACTGGGTGCAGGGCGTTCAGGCGTCGACGTGGGCCAACAACCCGCACAAC GACCGCACCAAGTACGACAAGTACGCCTACCCGCGCGGCAACATCATCGTCGGCGGCCAG GCCGTCACCAAGTCCGACTTCGTCAACGGGCTGCGCTACAAGTACAAGCGCTCCTGGGTG GACGGGCCGATGTACGCGCCGGTCACCGGCTACTCCTCGCAGACGTACGACGCCAGCCAG CTGGAGAAGCTGGAGGACGGCATCCTCTCCGGCACCGACTCGCGGCTGTTCTTCCGCAAC ACCCTGGACATGCTGACCGGCAAGCCCAAGCAGGGCGGCGACGTGGTCACCACCATCGAC CCCAAGGTGCAGAAGGCCGGCTTCGAGGGGCTCGGCAACAAGAAGGGCGCCGCGGTCGCC ATCGACCCGAAGACCGGGGCGATCCTCGGGCTGGTCTCCACCCCGTCCTACGACCCGGGC ACCTTCGCGGGCGGCACCAAGGACGACGAGAAGGCCTGGACGGCACTCGACAGCGACCCG AACAAGCCGATGCTGAACCGGGCGCTGCGCGAGACCTACCCGCCCGGCTCGACCTTCAAG CTGGTCACCGCGGCGACCGCGTTCGAGACCGGCAAGTACCAGAGCCCGTCGGACGTCACC GACACCCCGGACCAGTACATCCTGCCCGGCACCAGCACCCCGCTGATCAACGCCAGCCCC ACCGAGGACTGCGGGAACGCCACCGTGCAACACGCGATGGACCTGTCCTGCAACACGGTG TTCGGCAAGATGGGCGCCGAGCTGGGCGGCGACAAGCTGCGGGCGCAGGCCGAGAAGTTC GGCTTCAACAAGGAAGTGACCATCCCGATCCGGGCCGACGCCAGCCACTTCCCGTCCGGC TCCAAGCCCGACGGCACCGCGATGGACGCGATCGGCCAGCACGACACCCGGGCCACCCCG CTGCAGATGGCCATGGTGGCCGCCGCGATCGCCAACAACGGCTCGCTGATGGAGCCCTAC CTGGTCGCCCAGGAGGGCTCGGCCGGGAACGTGATCTCCACCCACACCGAGCACCAGCTG TCGCAGGCGGTCTCGCCGGCCACCGCGCAGAAGCTCCAGCAGCTGATGGAGTCGGTGGTG CAGCACGGCACCGGCACGAACGCGAAGATCCCGGGCGTGACGGTCGGCGGCAAGACCGGC ACCGCCCAGCACGGCCAGGACAACTCGGGTCTGCCGTTCGCCTGGTTCGTCTCCTACGCC AAGGGCCAGGACGGCAAGCAGGTCGCCGTCGCGGTGGTGGTCGAGGACGGCTCCAGCGAG GCGCAGCAGATCTCCGGCGGCTCGCTGGCCGCGCCGATCGCCAAGGCGATGATGCAGGCG GCGCTCGGGAAGTAG
You are now almost ready to build your remote `BLAST` query
The last two things you need to do are to consider the database we're going to search against, and the format you want the data returned in.
- You are going to query against the
pdbaadatabase (which contains amino acid sequences for the set of proteins with known structures) as this is small, and should return a result fairly quickly. This is a useful way to identify protein structures that may be useful for threading or other analyses (structural analyses will be covered in session 04). - You are going to request
"Text"format output, which will be easy for humans to read.
The search will return a special kind of object called a 'handle', which we still need to do some work with, to turn into a file, or read the results from.
NOTE: the remote search may take some time.
In [4]:
# Run a remote BLASTX search, with the penicillin-binding protein as a query,
# against the pdbaa database, getting text output
result_handle = NCBIWWW.qblast("blastx", "pdbaa", seq.format("fasta"),
format_type="Text")
Once the search has completed, you can read() the contents of the handle into a variable called output, and then inspect the contents using the print() function.
In [5]:
# Read the contents of the handle, and print to the cell
output = result_handle.read()
print(output)
<p><!--
QBlastInfoBegin
Status=READY
QBlastInfoEnd
--><p>
<PRE>
BLASTX 2.8.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: 9RXN2PZZ014
Database: PDB protein database
97,497 sequences; 24,642,510 total letters
Query= lcl|LISX01000001.1_cds_OKJ16671.1_31 [locus_tag=AMK19_00175]
[protein=penicillin-binding protein] [protein_id=OKJ16671.1]
[location=39730..41184]
Length=1455
Score E
Sequences producing significant alignments: (Bits) Value
3UN7_A Chain A, Crystal Structure Of Pbpa From Mycobacterium ... 297 2e-95
3LO7_A Chain A, Crystal structure of PBPA from Mycobacterium ... 295 2e-94
4MNR_A Chain A, Crystal Structure Of D,d-transpeptidase Domai... 216 2e-64
4RA7_A Chain A, Structure of a putative peptidoglycan glycosy... 128 6e-32
4N1X_A Chain A, Structure of a putative peptidoglycan glycosy... 128 9e-32
4JBF_A Chain A, Crystal structure of peptidoglycan glycosyltr... 128 9e-32
5E31_A Chain A, 2.3 Angstrom Crystal Structure Of The Monomer... 115 4e-27
4CPK_A Chain A, Crystal Structure Of Pbp2a Double Clinical Mu... 114 1e-26
1VQQ_A Chain A, Structure Of Penicillin Binding Protein 2a Fr... 114 1e-26
4BL2_A Chain A, Crystal Structure Of Pbp2a Clinical Mutant E1... 114 1e-26
3ZG5_A Chain A, Crystal Structure Of Pbp2a From Mrsa In Compl... 114 1e-26
4BL3_A Chain A, Crystal Structure Of Pbp2a Clinical Mutant N1... 112 4e-26
1MWS_A Chain A, Structure Of Nitrocefin Acyl-Penicillin Bindi... 112 5e-26
1MWR_A Chain A, Structure Of Semet Penicillin Binding Protein... 108 7e-25
3UE3_A Chain A, Crystal Structure Of Acinetobacter Baumanni Pbp3 93.2 8e-20
5TRO_A Chain A, 1.8 Angstrom Resolution Crystal Structure Of ... 91.7 3e-19
5UY7_A Chain A, Crystal Structure Of A Peptidoglycan Glycosyl... 90.9 5e-19
5LP4_A Chain A, Penicillin-Binding Protein (PBP2) from Helico... 89.0 2e-18
3EQV_A Chain A, Crystal Structure Of Penicillin-binding Prote... 87.8 5e-18
3EQU_A Chain A, Crystal Structure Of Penicillin-Binding Prote... 87.0 9e-18
5KSH_A Chain A, Crystal Structure Of Penicillin-binding Prote... 85.9 2e-17
4L0L_A Chain A, Crystal Structure Of P.aeruginosa Pbp3 In Com... 78.2 5e-15
3PBN_A Chain A, Crystal Structure Of Apo Pbp3 From Pseudomona... 77.8 7e-15
4BJP_A Chain A, Crystal Structure Of E. Coli Penicillin Bindi... 77.8 7e-15
4FSF_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa P... 77.8 7e-15
4WEK_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa P... 77.8 7e-15
3OC2_A Chain A, Crystal Structure Of Penicillin-binding Prote... 77.8 9e-15
4ZTK_A Chain A, Transpeptidase Domain Of Ftsi4 D,d-transpepti... 64.3 6e-11
4OVD_A Chain A, Crystal structure of a putative peptidoglycan... 65.5 7e-11
4U3T_A Chain A, Crystal Structure Of The Transpeptidase Domai... 60.1 2e-09
3ZG8_B Chain B, Crystal Structure Of Penicillin Binding Prote... 60.5 2e-09
2WAF_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From S... 58.2 1e-08
2V2F_F Chain F, Crystal Structure Of Pbp1a From Drug-resistan... 57.8 1e-08
2C5W_B Chain B, Penicillin-Binding Protein 1a (Pbp-1a) Acyl-E... 55.8 6e-08
2ZC5_B Chain B, Penicillin-Binding Protein 1a (Pbp 1a) Acyl-E... 55.1 1e-07
2C6W_B Chain B, Penicillin-Binding Protein 1a (Pbp-1a) From S... 54.7 1e-07
3ZG7_B Chain B, Crystal Structure Of Penicillin-binding Prote... 53.9 3e-07
2WAD_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From S... 53.5 4e-07
2WAE_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From S... 53.1 6e-07
3UDF_A Chain A, Crystal Structure Of Apo Pbp1a From Acinetoba... 50.8 3e-06
1K25_A Chain A, PBP2x from a Highly Penicillin-resistant Stre... 49.7 6e-06
5U47_A Chain A, 1.95 Angstrom Resolution Crystal Structure Of... 48.9 1e-05
1RP5_A Chain A, Pbp2x From Streptococcus Pneumoniae Strain 52... 47.4 4e-05
4OON_A Chain A, Crystal Structure Of Pbp1a In Complex With Co... 46.6 7e-05
1PYY_A Chain A, Double Mutant Pbp2x T338aM339F FROM STREPTOCO... 43.9 4e-04
1PMD_A Chain A, Penicillin-Binding Protein 2x (Pbp-2x) 43.5 5e-04
1QME_A Chain A, Penicillin-binding Protein 2x (pbp-2x) 43.1 7e-04
4YE5_A Chain A, The Crystal Structure Of A Peptidoglycan Synt... 42.7 8e-04
3DWK_A Chain A, Identification Of Dynamic Structural Motifs I... 42.4 0.001
2Z2L_B Chain B, Penicillin-binding Protein 2x (pbp2x) From St... 40.4 0.004
ALIGNMENTS
>3UN7_A Chain A, Crystal Structure Of Pbpa From Mycobacterium Tuberculosis
3UN7_B Chain B, Crystal Structure Of Pbpa From Mycobacterium Tuberculosis
3UPN_A Chain A, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Imipenem Acyl-Enzyme Complex
3UPN_B Chain B, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Imipenem Acyl-Enzyme Complex
3UPO_A Chain A, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Penicillin G Acyl-Enzyme Complex
3UPO_B Chain B, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Penicillin G Acyl-Enzyme Complex
3UPP_A Chain A, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Ceftrixaone Acyl-Enzyme Complex
3UPP_B Chain B, Structure Of Penicillin-Binding Protein A From M. Tuberculosis:
Ceftrixaone Acyl-Enzyme Complex
Length=462
Score = 297 bits (760), Expect = 2e-95, Method: Compositional matrix adjust.
Identities = 192/461 (42%), Positives = 265/461 (57%), Gaps = 25/461 (5%)
Frame = +1
Query 109 NPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTY 288
+P N R D+Y+ RG I GGQ + S +G R+++ R + + +YAPVTG+ S Y
Sbjct 8 DPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDG-RFRFLRVYPNPEVYAPVTGFYSLRY 66
Query 289 DASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGL----- 453
++ LE+ ED IL+G+D RLF R D TG+ +GG+V TTI+P++Q+AG++ +
Sbjct 67 SSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCY 126
Query 454 GNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDE-KAWTALDSDPNKPMLNRALR 630
G KGA VA++P TG IL LVS+PSYDP A + + +AW L +P P+ NRA+
Sbjct 127 GPCKGAVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAIS 186
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLIN--ASPTEDCGNAT 804
ETYPPGSTFK++T A A G ++ +T P LPG++ L N +P D +
Sbjct 187 ETYPPGSTFKVITTAAALAAGATET-EQLTAAPT-IPLPGSTAQLENYGGAPCGDEPTVS 244
Query 805 VQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTA- 981
++ A SCNT F ++G G D LR+ A FG + P + G PD A
Sbjct 245 LREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPR-PTPLQVAESTVGPIPDSAAL 303
Query 982 -MDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ-EGSAGNVISTHTEHQLSQAVS 1155
M +IGQ D TPL A +AA IAN G M PYLV +G IST +Q +AVS
Sbjct 304 GMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVRYQQRRAVS 363
Query 1156 PATAQKLQQLM---ESVVQHGTGTNAKIPGVTVGGKTGTAQHGQD-NSGLPFAWFVSYAK 1323
P A KL +LM E V Q IPGV + KTGTA+HG D P AW++++A
Sbjct 364 PQVAAKLTELMVGAEKVAQQ----KGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAP 419
Query 1324 GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
Q K VAVAV+VE+G+ +GG+LAAPI +A+++AAL
Sbjct 420 AQAPK-VAVAVLVENGADRLSA-TGGALAAPIGRAVIEAAL 458
>3LO7_A Chain A, Crystal structure of PBPA from Mycobacterium tuberculosis
3LO7_B Chain B, Crystal structure of PBPA from Mycobacterium tuberculosis
Length=483
Score = 295 bits (755), Expect = 2e-94, Method: Compositional matrix adjust.
Identities = 192/461 (42%), Positives = 265/461 (57%), Gaps = 25/461 (5%)
Frame = +1
Query 109 NPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTY 288
+P N R D+Y+ RG I GGQ + S +G R+++ R + + +YAPVTG+ S Y
Sbjct 29 DPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDG-RFRFLRVYPNPEVYAPVTGFYSLRY 87
Query 289 DASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGL----- 453
++ LE+ ED IL+G+D RLF R D TG+ +GG+V TTI+P++Q+AG++ +
Sbjct 88 SSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCY 147
Query 454 GNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDE-KAWTALDSDPNKPMLNRALR 630
G KGA VA++P TG IL LVS+PSYDP A + + +AW L +P P+ NRA+
Sbjct 148 GPCKGAVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAIS 207
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLIN--ASPTEDCGNAT 804
ETYPPGSTFK++T A A G ++ +T P LPG++ L N +P D +
Sbjct 208 ETYPPGSTFKVITTAAALAAGATET-EQLTAAPT-IPLPGSTAQLENYGGAPCGDEPTVS 265
Query 805 VQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTA- 981
++ A SCNT F ++G G D LR+ A FG + P + G PD A
Sbjct 266 LREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPR-PTPLQVAESTVGPIPDSAAL 324
Query 982 -MDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ-EGSAGNVISTHTEHQLSQAVS 1155
M +IGQ D TPL A +AA IAN G M PYLV +G IST +Q +AVS
Sbjct 325 GMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVS 384
Query 1156 PATAQKLQQLM---ESVVQHGTGTNAKIPGVTVGGKTGTAQHGQD-NSGLPFAWFVSYAK 1323
P A KL +LM E V Q IPGV + KTGTA+HG D P AW++++A
Sbjct 385 PQVAAKLTELMVGAEKVAQQ----KGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAP 440
Query 1324 GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
Q K VAVAV+VE+G+ +GG+LAAPI +A+++AAL
Sbjct 441 AQAPK-VAVAVLVENGADRLSA-TGGALAAPIGRAVIEAAL 479
>4MNR_A Chain A, Crystal Structure Of D,d-transpeptidase Domain Of Peptidoglycan
Glycosyltransferase From Eggerthella Lenta
Length=457
Score = 216 bits (550), Expect = 2e-64, Method: Compositional matrix adjust.
Identities = 167/457 (37%), Positives = 236/457 (52%), Gaps = 54/457 (12%)
Frame = +1
Query 85 VQASTWANNPHNDRTKYDKYAYPRGNI-----IVGGQAVTKSDFVNGLRYKYKRSWVDGP 249
VQA + N P N+ T + RG I +V ++V + D Y+R + G
Sbjct 4 VQADYYQNXPGNNHTLAKEARSERGTIATYDGVVLARSVKEEDGT------YEREYPAGD 57
Query 250 MYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKV 429
+ + V GYSS + S +EK + L G ++ + + L+ G G DV T++ K+
Sbjct 58 LASHVVGYSSPQFGNSGIEKAYNDTLKGEENFASWTDVLNSFAGIGTAGNDVTLTLNSKI 117
Query 430 QKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
Q+A + L +KGA V DP TGAIL S P+Y+ FA E+A +++P+
Sbjct 118 QQAAQDALAGRKGACVVXDPDTGAILAXASAPTYNAADFAAVI---EQA----NANPDDS 170
Query 610 ML-NRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTE 786
L +RA Y PGSTFK+VT ATA E DV + PGT + NA+ +
Sbjct 171 TLVDRAAGSLYAPGSTFKIVTLATALE-------DDVAGEDTVFSSPGTXE-IGNATVSN 222
Query 787 ----DCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP 954
+ G+ T+ A +LS NTVFG++G E G DKL A AE FGFNKE+ P+ S P
Sbjct 223 FNKANYGSLTLAQATELSSNTVFGQLGVEXGADKLVAGAESFGFNKEIDFPLYTPESLXP 282
Query 955 SGS----KPDGTAMDAIGQ--HDT-----------RATPLQMAMVAAAIANNGSLMEPYL 1083
S P A A G+ DT +AT L+ A V AIAN+G + +PYL
Sbjct 283 SAEDLQKSPWELAWAAAGEPVGDTTRPGRESPAGPQATVLEXAXVGTAIANDGVIXQPYL 342
Query 1084 VAQEGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTG 1260
V +A G + + +L QAVS TA +++ ++ VVQ+GTGT A IPG+ V GKTG
Sbjct 343 VDSVNNANGERSFSASPTKLXQAVSKTTAGRVRDVLLGVVQNGTGTAAAIPGIDVAGKTG 402
Query 1261 TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDG 1371
TA+ N +WFV A +D +V VA+V+EDG
Sbjct 403 TAEKENGND----SWFVGXAPAED-PRVVVAIVIEDG 434
>4RA7_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with nafcillin
4RA7_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with nafcillin
Length=453
Score = 128 bits (321), Expect = 6e-32, Method: Compositional matrix adjust.
Identities = 131/434 (30%), Positives = 196/434 (45%), Gaps = 40/434 (9%)
Frame = +1
Query 112 PHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYD 291
P N+ T K AY + I+ VT ++ V Y R++ + GY S Y
Sbjct 13 PTNNHT-IAKSAYVQRGAIITSDGVTLAESVKQDDGTYVRNYPHDGXASHTVGYISTQYG 71
Query 292 ASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGA 471
+ +E + L+G +R+ L G G VV TI+ + Q L G+
Sbjct 72 TAGIESSXNETLTGHADHSDWRSALYSXAGINTTGSSVVLTINSQXQAVAEAALQGYSGS 131
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
V DP TGA+L S+PSY T ++S +++R + Y PGS
Sbjct 132 IVVXDPSTGAVLAKASSPSYTHAELG----------TIIESGTGSQLVDRTTQALYSPGS 181
Query 652 TFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTST---PLINASPTEDCGNATVQHAMD 822
+FK VT A +T K T Y PGT I+ ED G ++ A
Sbjct 182 SFKTVTLAAGIDTHK-------TTLDTTYSAPGTXEIGGGTIHNYANEDXGTIPLREAFA 234
Query 823 LSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPD------GTAM 984
S NT ++G LG D L + A FG+ + S P+ ++ +
Sbjct 235 RSSNTALAQLGVALGADNLVSYARAFGYGTALGQDFSTTPSLXPNPAEXTTWELAWASCG 294
Query 985 DAIGQHDTRATP----LQMAMVAAAIANNGSLMEPYLVAQEGS-AGNVISTHTEHQLSQA 1149
+G+H + A P Q A++AAAIAN G + PY+V + S G V+ST + L QA
Sbjct 295 LPVGEHASPAGPQTTVXQNAVIAAAIANGGVVXNPYIVDRVLSPEGAVVSTTSPKSLGQA 354
Query 1150 VSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTA--QHGQDNSGLPFAWFVSYAK 1323
VS TA ++++ VV+ GTG A++PGV + GKTGTA ++G NS +F+ +A
Sbjct 355 VSADTAAQVREAXLGVVESGTGXGARVPGVKIAGKTGTADVENGNFNS-----FFIGFAP 409
Query 1324 GQDGKQVAVAVVVE 1365
D + V+VV+E
Sbjct 410 -YDHPTLVVSVVIE 422
>4N1X_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with penicillin G
4N1X_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with penicillin G
Length=472
Score = 128 bits (321), Expect = 9e-32, Method: Compositional matrix adjust.
Identities = 132/445 (30%), Positives = 201/445 (45%), Gaps = 40/445 (9%)
Frame = +1
Query 79 QGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSWVDGPMYA 258
+G + + +N N+ T K AY + I+ VT ++ V Y R++ +
Sbjct 21 KGTENLYFQSNATNNHT-IAKSAYVQRGAIITSDGVTLAESVKQDDGTYVRNYPHDGXAS 79
Query 259 PVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKA 438
GY S Y + +E + L+G +R+ L G G VV TI+ + Q
Sbjct 80 HTVGYISTQYGTAGIESSXNETLTGHADHSDWRSALYSXAGINTTGSSVVLTINSQXQAV 139
Query 439 GFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLN 618
L G+ V DP TGA+L S+PSY T ++S +++
Sbjct 140 AEAALQGYSGSIVVXDPSTGAVLAKASSPSYTHAELG----------TIIESGTGSQLVD 189
Query 619 RALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTST---PLINASPTED 789
R + Y PGS+FK VT A +T K T Y PGT I+ ED
Sbjct 190 RTTQALYSPGSSFKTVTLAAGIDTHK-------TTLDTTYSAPGTXEIGGGTIHNYANED 242
Query 790 CGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKP 969
G ++ A S NT ++G LG D L + A FG+ + S P+ ++
Sbjct 243 XGTIPLREAFARSSNTALAQLGVALGADNLVSYARAFGYGTALGQDFSTTPSLXPNPAEX 302
Query 970 D------GTAMDAIGQHDTRATP----LQMAMVAAAIANNGSLMEPYLVAQEGS-AGNVI 1116
+ +G+H + A P Q A++AAAIAN G + PY+V + S G V+
Sbjct 303 TTWELAWASCGLPVGEHASPAGPQTTVXQNAVIAAAIANGGVVXNPYIVDRVLSPEGAVV 362
Query 1117 STHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTA--QHGQDNSG 1290
ST + L QAVS TA ++++ VV+ GTG A++PGV + GKTGTA ++G NS
Sbjct 363 STTSPKSLGQAVSADTAAQVREAXLGVVESGTGXGARVPGVKIAGKTGTADVENGNFNS- 421
Query 1291 LPFAWFVSYAKGQDGKQVAVAVVVE 1365
+F+ +A D + V+VV+E
Sbjct 422 ----FFIGFAP-YDHPTLVVSVVIE 441
>4JBF_A Chain A, Crystal structure of peptidoglycan glycosyltransferase
from Atopobium parvulum DSM 20469.
4JBF_B Chain B, Crystal structure of peptidoglycan glycosyltransferase
from Atopobium parvulum DSM 20469.
4QJG_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with penicillin V
4QJG_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with penicillin V
4R0Q_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cephalothin
4R0Q_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cephalothin
4R1G_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cloxacillin
4R1G_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cloxacillin
4R23_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with dicloxacillin
4R23_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with dicloxacillin
4R3J_A Chain A, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cefapirin
4R3J_B Chain B, Structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum in complex with cefapirin
Length=482
Score = 128 bits (321), Expect = 9e-32, Method: Compositional matrix adjust.
Identities = 131/434 (30%), Positives = 196/434 (45%), Gaps = 40/434 (9%)
Frame = +1
Query 112 PHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYD 291
P N+ T K AY + I+ VT ++ V Y R++ + GY S Y
Sbjct 42 PTNNHT-IAKSAYVQRGAIITSDGVTLAESVKQDDGTYVRNYPHDGXASHTVGYISTQYG 100
Query 292 ASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGA 471
+ +E + L+G +R+ L G G VV TI+ + Q L G+
Sbjct 101 TAGIESSXNETLTGHADHSDWRSALYSXAGINTTGSSVVLTINSQXQAVAEAALQGYSGS 160
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
V DP TGA+L S+PSY T ++S +++R + Y PGS
Sbjct 161 IVVXDPSTGAVLAKASSPSYTHAELG----------TIIESGTGSQLVDRTTQALYSPGS 210
Query 652 TFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTST---PLINASPTEDCGNATVQHAMD 822
+FK VT A +T K T Y PGT I+ ED G ++ A
Sbjct 211 SFKTVTLAAGIDTHK-------TTLDTTYSAPGTXEIGGGTIHNYANEDXGTIPLREAFA 263
Query 823 LSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPD------GTAM 984
S NT ++G LG D L + A FG+ + S P+ ++ +
Sbjct 264 RSSNTALAQLGVALGADNLVSYARAFGYGTALGQDFSTTPSLXPNPAEXTTWELAWASCG 323
Query 985 DAIGQHDTRATP----LQMAMVAAAIANNGSLMEPYLVAQEGS-AGNVISTHTEHQLSQA 1149
+G+H + A P Q A++AAAIAN G + PY+V + S G V+ST + L QA
Sbjct 324 LPVGEHASPAGPQTTVXQNAVIAAAIANGGVVXNPYIVDRVLSPEGAVVSTTSPKSLGQA 383
Query 1150 VSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTA--QHGQDNSGLPFAWFVSYAK 1323
VS TA ++++ VV+ GTG A++PGV + GKTGTA ++G NS +F+ +A
Sbjct 384 VSADTAAQVREAXLGVVESGTGXGARVPGVKIAGKTGTADVENGNFNS-----FFIGFAP 438
Query 1324 GQDGKQVAVAVVVE 1365
D + V+VV+E
Sbjct 439 -YDHPTLVVSVVIE 451
>5E31_A Chain A, 2.3 Angstrom Crystal Structure Of The Monomeric Form
Of Penicillin Binding Protein 2 Prime From Enterococcus Faecium.
Length=644
Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust.
Identities = 98/343 (29%), Positives = 158/343 (46%), Gaps = 29/343 (8%)
Frame = +1
Query 319 GILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTG 498
G LS TD+ + L + + + G D+ TID K QK F+ LG K G+ VA PKTG
Sbjct 283 GKLSITDADGVEKKVL--IEHEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTG 340
Query 499 AILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAAT 678
+L L S+PSYDP G ++ + A + +P +P ++R Y PGSTFK +TAA
Sbjct 341 DLLALASSPSYDPNKXTNGISQED--YKAYEENPEQPFISR-FATGYAPGSTFKXITAAI 397
Query 679 AFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGA 858
+ G P++V +S + D ++ A+ S N +
Sbjct 398 GLDNGTI-DPNEVLTINGLKWQKDSSWGSYQVTRVSDVSQVDLKTALIYSDNIYXAQETL 456
Query 859 ELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAI------GQHDTRATP 1020
+ G K R +KF F +++ +PI + + S D D + GQ + P
Sbjct 457 KXGEKKFRTGLDKFIFGEDLDLPISXNPAQI---SNEDSFNSDILLADTGYGQGELLINP 513
Query 1021 LQMAMVAAAIANNGSLMEPYLVAQEGSAG--NVISTHTEHQLSQAVSPATAQKLQQLMES 1194
+Q A + ANNG+L+ P L+A + + NVI Q + P L+++++
Sbjct 514 IQQAAXYSVFANNGTLVYPKLIADKETKDKKNVIG----ETAVQTIVP----DLREVVQD 565
Query 1195 VVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLPFAWFVSY 1317
V +GT + G+ + KTGTA+ QD G ++ ++
Sbjct 566 V--NGTAHSLSALGIPLAAKTGTAEIKEKQDEKGKENSFLFAF 606
>4CPK_A Chain A, Crystal Structure Of Pbp2a Double Clinical Mutant N146k-
E150k From Mrsa
4CPK_B Chain B, Crystal Structure Of Pbp2a Double Clinical Mutant N146k-
E150k From Mrsa
Length=643
Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 107/384 (28%), Positives = 165/384 (43%), Gaps = 59/384 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 258 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 314
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 315 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 371
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 372 ITTSPGSTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 424
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 425 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 477
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 478 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 528
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLP 1296
+ +S L M+ VV + + GK+GTA+ Q +G
Sbjct 529 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ 588
Query 1297 FAWFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 589 IGWFISYDK--DNPNMMMAINVKD 610
>1VQQ_A Chain A, Structure Of Penicillin Binding Protein 2a From Methicillin
Resistant Staphylococcus Aureus Strain 27r At 1.80 A
Resolution.
1VQQ_B Chain B, Structure Of Penicillin Binding Protein 2a From Methicillin
Resistant Staphylococcus Aureus Strain 27r At 1.80 A
Resolution.
1MWU_A Chain A, Structure Of Methicillin Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.60 A Resolution.
1MWU_B Chain B, Structure Of Methicillin Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.60 A Resolution.
4DKI_A Chain A, Structural Insights Into The Anti- Methicillin-Resistant
Staphylococcus Aureus (Mrsa) Activity Of Ceftobiprole
4DKI_B Chain B, Structural Insights Into The Anti- Methicillin-Resistant
Staphylococcus Aureus (Mrsa) Activity Of Ceftobiprole
Length=646
Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 107/384 (28%), Positives = 165/384 (43%), Gaps = 59/384 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 261 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 317
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 318 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 374
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 375 ITTSPGSTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 427
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 428 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 480
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 481 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 531
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLP 1296
+ +S L M+ VV + + GK+GTA+ Q +G
Sbjct 532 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ 591
Query 1297 FAWFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 592 IGWFISYDK--DNPNMMMAINVKD 613
>4BL2_A Chain A, Crystal Structure Of Pbp2a Clinical Mutant E150k From
Mrsa
4BL2_B Chain B, Crystal Structure Of Pbp2a Clinical Mutant E150k From
Mrsa
Length=643
Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 107/384 (28%), Positives = 165/384 (43%), Gaps = 59/384 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 258 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 314
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 315 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 371
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 372 ITTSPGSTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 424
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 425 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 477
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 478 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 528
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLP 1296
+ +S L M+ VV + + GK+GTA+ Q +G
Sbjct 529 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ 588
Query 1297 FAWFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 589 IGWFISYDK--DNPNMMMAINVKD 610
>3ZG5_A Chain A, Crystal Structure Of Pbp2a From Mrsa In Complex With
Peptidoglycan Analogue At Allosteric
3ZG5_B Chain B, Crystal Structure Of Pbp2a From Mrsa In Complex With
Peptidoglycan Analogue At Allosteric
3ZG0_A Chain A, Crystal Structure Of Ceftaroline Acyl-pbp2a From Mrsa
With Non-covalently Bound Ceftaroline And Muramic Acid At
Allosteric Site Obtained By Cocrystallization
3ZG0_B Chain B, Crystal Structure Of Ceftaroline Acyl-pbp2a From Mrsa
With Non-covalently Bound Ceftaroline And Muramic Acid At
Allosteric Site Obtained By Cocrystallization
3ZFZ_A Chain A, Crystal Structure Of Ceftaroline Acyl-pbp2a From Mrsa
With Non-covalently Bound Ceftaroline And Muramic Acid At
Allosteric Site Obtained By Soaking
3ZFZ_B Chain B, Crystal Structure Of Ceftaroline Acyl-pbp2a From Mrsa
With Non-covalently Bound Ceftaroline And Muramic Acid At
Allosteric Site Obtained By Soaking
4CJN_A Chain A, Crystal Structure Of Pbp2a From Mrsa In Complex With
Quinazolinone Ligand
4CJN_B Chain B, Crystal Structure Of Pbp2a From Mrsa In Complex With
Quinazolinone Ligand
5M18_A Chain A, Crystal structure of PBP2a from MRSA in the presence
of Cefepime ligand
5M18_B Chain B, Crystal structure of PBP2a from MRSA in the presence
of Cefepime ligand
5M19_A Chain A, Crystal structure of PBP2a from MRSA in the presence
of Oxacillin ligand
5M19_B Chain B, Crystal structure of PBP2a from MRSA in the presence
of Oxacillin ligand
5M1A_A Chain A, Crystal structure of PBP2a from MRSA in the presence
of Ceftazidime ligand
5M1A_B Chain B, Crystal structure of PBP2a from MRSA in the presence
of Ceftazidime ligand
Length=642
Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust.
Identities = 107/384 (28%), Positives = 165/384 (43%), Gaps = 59/384 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 257 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 313
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 314 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 370
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 371 ITTSPGSTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 423
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 424 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 476
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 477 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 527
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLP 1296
+ +S L M+ VV + + GK+GTA+ Q +G
Sbjct 528 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ 587
Query 1297 FAWFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 588 IGWFISYDK--DNPNMMMAINVKD 609
>4BL3_A Chain A, Crystal Structure Of Pbp2a Clinical Mutant N146k From
Mrsa
4BL3_B Chain B, Crystal Structure Of Pbp2a Clinical Mutant N146k From
Mrsa
Length=640
Score = 112 bits (281), Expect = 4e-26, Method: Compositional matrix adjust.
Identities = 106/382 (28%), Positives = 163/382 (43%), Gaps = 58/382 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 258 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 314
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 315 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 371
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 372 ITTSPGSTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 424
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 425 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 477
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 478 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 528
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSGLPFA 1302
+ +S L M+ VV + + GK+GTA+ G
Sbjct 529 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKM-KQGRQIG 587
Query 1303 WFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 588 WFISYDK--DNPNMMMAINVKD 607
>1MWS_A Chain A, Structure Of Nitrocefin Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.00 A Resolution.
1MWS_B Chain B, Structure Of Nitrocefin Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.00 A Resolution.
1MWT_A Chain A, Structure Of Penicillin G Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.45 A Resolution.
1MWT_B Chain B, Structure Of Penicillin G Acyl-Penicillin Binding Protein
2a From Methicillin Resistant Staphylococcus Aureus Strain
27r At 2.45 A Resolution.
Length=646
Score = 112 bits (280), Expect = 5e-26, Method: Compositional matrix adjust.
Identities = 106/384 (28%), Positives = 164/384 (43%), Gaps = 59/384 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 261 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 317
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
+ N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 318 MKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEE--YNKLTEDKKEPLLNK-FQ 374
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PG T K++TA + D Y + G S N + E
Sbjct 375 ITTSPGXTQKILTAMIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 427
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 428 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDI-------PSDYPFYNAQ 480
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVIST 1122
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ T
Sbjct 481 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLK---------DT 531
Query 1123 HTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HGQDNSGLP 1296
+ +S L M+ VV + + GK+GTA+ Q +G
Sbjct 532 KNKVWKKNIISKENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQ 591
Query 1297 FAWFVSYAKGQDGKQVAVAVVVED 1368
WF+SY K D + +A+ V+D
Sbjct 592 IGWFISYDK--DNPNMMMAINVKD 613
>1MWR_A Chain A, Structure Of Semet Penicillin Binding Protein 2a From
Methicillin Resistant Staphylococcus Aureus Strain 27r (Trigonal
Form) At 2.45 A Resolution.
1MWR_B Chain B, Structure Of Semet Penicillin Binding Protein 2a From
Methicillin Resistant Staphylococcus Aureus Strain 27r (Trigonal
Form) At 2.45 A Resolution.
Length=646
Score = 108 bits (271), Expect = 7e-25, Method: Compositional matrix adjust.
Identities = 105/387 (27%), Positives = 165/387 (43%), Gaps = 71/387 (18%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFRNTL----------DMLTGKPKQGGDVVTTIDPKVQKAGFEG 450
LEKL D L D +R T+ ++ K K G D+ TID KVQK+ +
Sbjct 261 LEKLYDKKLQHEDG---YRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNN 317
Query 451 LGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALR 630
N G+ AI P+TG +L LVSTPSYD F G ++E + L D +P+LN+ +
Sbjct 318 XKNDYGSGTAIHPQTGELLALVSTPSYDVYPFXYGXSNEE--YNKLTEDKKEPLLNK-FQ 374
Query 631 ETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG------TSTPLINASPTEDC 792
T PGST K++TA + D Y + G S N + E
Sbjct 375 ITTSPGSTQKILTAXIGLN-------NKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV 427
Query 793 -GNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFP----- 954
GN ++ A++ S N F ++ ELG K +K G +++ S +P
Sbjct 428 NGNIDLKQAIESSDNIFFARVALELGSKKFEKGXKKLGVGEDI-------PSDYPFYNAQ 480
Query 955 -SGSKPDGTAMDA---IGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGS---AGNV 1113
S D + A GQ + P+Q+ + +A+ NNG++ P+L+ + N+
Sbjct 481 ISNKNLDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNI 540
Query 1114 ISTHTEHQLS----QAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ--HG 1275
IS + L+ Q V+ + + + +++ GK+GTA+
Sbjct 541 ISKENINLLTDGXQQVVNKTHKEDIYRSYANLI----------------GKSGTAELKXK 584
Query 1276 QDNSGLPFAWFVSYAKGQDGKQVAVAV 1356
Q +G WF+SY K A+ V
Sbjct 585 QGETGRQIGWFISYDKDNPNXXXAINV 611
>3UE3_A Chain A, Crystal Structure Of Acinetobacter Baumanni Pbp3
Length=554
Score = 93.2 bits (230), Expect = 8e-20, Method: Compositional matrix adjust.
Identities = 104/372 (28%), Positives = 163/372 (44%), Gaps = 55/372 (15%)
Frame = +1
Query 388 KQGGDVVTTIDPKVQKAGFE-----GLGN--KKGAAVAIDPKTGAILGLVSTPSYDPGTF 546
+ G ++ +ID ++Q + G+ N + A+A+D KTG IL + S PSY+P
Sbjct 200 ESGENITLSIDSRLQYIMYRELTAAGVANNARSATAIAVDVKTGEILAMTSWPSYNPNDK 259
Query 547 AGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDT 726
G + D M NR + + PGST K T A A E+G+Y +P+ + +T
Sbjct 260 GGLSNKDA-------------MRNRGAIDMFEPGSTMKPFTVAAALESGQY-TPNSIVNT 305
Query 727 PDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGF 906
+ G T T + G T+ + S N K+ L + L + + GF
Sbjct 306 APGTMRLGWHT----IRDTHNYGALTLTGIIVKSSNVGSAKLALSLPKEALPSFFRRAGF 361
Query 907 NKEVTIPIRAD-ASHFPSGSKPDGTAMDAIGQ-HDTRATPLQMAMVAAAIANNGSLMEPY 1080
+ + + A S K + + + + + AT LQ+A A +AN+G M
Sbjct 362 GQSSDVKFPGESAGLLYSADKLNSSQLGTMAYGYGLNATILQLAQGYAMLANHGVEMP-- 419
Query 1081 LVAQEGSAGNVISTHTEHQL---SQAVSPATAQKLQQLMESV-VQHGTGTNAKIPGVTVG 1248
+S H Q+ Q + P A ++ ++E V + GT A IPG VG
Sbjct 420 -----------LSLHKLDQVPEGRQVLDPKIADQVLMMLEQVTLPGGTAKQAVIPGYRVG 468
Query 1249 GKTGTA------QHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLA 1410
GKTGTA + G NS A F A D + +A+ VVVE+ + GG +A
Sbjct 469 GKTGTAHKLRADRKGYSNSEY-RALFAGVAPVSDPR-LAMIVVVENPQG---RYYGGLVA 523
Query 1411 APIAKAMMQAAL 1446
AP+ +MQ +L
Sbjct 524 APVFAKIMQESL 535
>5TRO_A Chain A, 1.8 Angstrom Resolution Crystal Structure Of Dimerization
And Transpeptidase Domains (residues 39-608) Of Penicillin-binding
Protein 1 From Staphylococcus Aureus.
5TRO_B Chain B, 1.8 Angstrom Resolution Crystal Structure Of Dimerization
And Transpeptidase Domains (residues 39-608) Of Penicillin-binding
Protein 1 From Staphylococcus Aureus.
Length=573
Score = 91.7 bits (226), Expect = 3e-19, Method: Compositional matrix adjust.
Identities = 117/438 (27%), Positives = 178/438 (41%), Gaps = 67/438 (15%)
Frame = +1
Query 226 KRSWVDGPMYAPVTGYSSQTYDASQL------EKLEDGILSGTDSRLFFRNTL------- 366
+R + +G + + G + + D +L EK+ D LSG+ L + + +
Sbjct 133 ERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPN 192
Query 367 DMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTP 525
+PK+G DV TID +Q E L K AV D KTG IL P
Sbjct 193 TKKEKQPKRGDDVHLTIDSNIQVFVEEALDGXVERYQPKDLFAVVXDAKTGEILAYSQRP 252
Query 526 SYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQS 705
+++P T KD K W N + TY PGSTFK A A + G +
Sbjct 253 TFNPET----GKDFGKKWA-----------NDLYQNTYEPGSTFKSYGLAAAIQEGAFD- 296
Query 706 PSDVTDTPDQYILPGTSTPLINASPTED-----CGNATVQHAMDLSCNTVFGKMGAELGG 870
PD+ G I S D G S NT+ + +G
Sbjct 297 -------PDKKYKSGHRD--IXGSRISDWNRVGWGEIPXSLGFTYSSNTLXXHLQDLVGA 347
Query 871 DKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAI 1050
DK ++ E+FGF K +A S + GQ T TP+Q +A
Sbjct 348 DKXKSWYERFGFGKSTKGXFDGEAPGQIGWSNELQQKTSSFGQ-STTVTPVQXLQAQSAF 406
Query 1051 ANNGSLMEPYLVAQEGSAGNVISTHT----EHQLS-QAVSPATAQKLQQLMESVV--QHG 1209
N+G+ ++P+ V S N +S + Q++ + ++ TA+K+++ ++ VV +
Sbjct 407 FNDGNXLKPWFV---NSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKS 463
Query 1210 TGTNAKIPGVTVGGKTGTAQHGQDNSG----LPFAWFVSYAKGQDGKQVAVAVVVEDGSS 1377
N +I G V GKTGTAQ N G P +FVS+ D + V+V G S
Sbjct 464 HAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFX--GDAPKKNPKVIVYAGXS 521
Query 1378 EAQQISGGSLAAPIAKAM 1431
AQ+ + ++KA
Sbjct 522 LAQKNDQEAYELGVSKAF 539
>5UY7_A Chain A, Crystal Structure Of A Peptidoglycan Glycosyltransferase
From Burkholderia Ambifaria
Length=579
Score = 90.9 bits (224), Expect = 5e-19, Method: Compositional matrix adjust.
Identities = 116/443 (26%), Positives = 192/443 (43%), Gaps = 74/443 (17%)
Frame = +1
Query 214 RYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLED-GILSGTDS-RLFFRNTLDMLTGK- 384
R +YKR + +G + A + G+++ + + +L D +LSGT R ++ L +
Sbjct 124 RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLSGTSGVRRVIKDRLGHIVEDV 183
Query 385 -----PKQGGDVVTTIDPKVQ-------KAGFEGLGNKKGAAVAIDPKTGAILGLVSTPS 528
P G DV +ID K+Q KA E K GAA+ +D +TG +L LV+ P+
Sbjct 184 AEQVPPHNGTDVGLSIDSKIQYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPT 243
Query 529 YDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSP 708
Y+P +D T + + NR L + + PGS K T + A + +
Sbjct 244 YNP--------NDRTRLTG------EQLRNRILTDVFEPGSIMKPFTVSLALDLHRV--- 286
Query 709 SDVTDTPDQYILPGTSTPLINASPTED---CGNATVQHAMDLSCNTVFGKMGAELGGDKL 879
TP+ + G +++ +P D G TV + S N K+ + +++
Sbjct 287 -----TPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEM 341
Query 880 --------RAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAM 1035
QA K GF +R + S + + M + + Q+A
Sbjct 342 WNMYTSIGLGQAPKVGFPGAAAGRLRP----WKSWRRIEQATMSY--GYGLSVSLFQLAR 395
Query 1036 VAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQL--SQAVSPATAQKLQQLMESVVQH- 1206
AIA++G +M + T Q+ +Q +P TA++++ ++E+VV
Sbjct 396 AYTAIAHDGEMMPV----------TIFKTDPNQQITGTQVFTPTTAREVRTMLETVVAPG 445
Query 1207 GTGTNAKIPGVTVGGKTGTA--QHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVED---G 1371
GT +A +PG VGGK+GTA G + A FV A ++ VAV V++ G
Sbjct 446 GTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAP-MPNPRIVVAVSVDEPTAG 504
Query 1372 SSEAQQISGGSLAAPIAKAMMQA 1440
S Q+SG +A IA M+A
Sbjct 505 SHFGGQVSGPVFSA-IAGDTMRA 526
>5LP4_A Chain A, Penicillin-Binding Protein (PBP2) from Helicobacter
pylori
5LP4_B Chain B, Penicillin-Binding Protein (PBP2) from Helicobacter
pylori
5LP5_A Chain A, Complex Between Penicillin-binding Protein (pbp2) And
Mrec From Helicobacter Pylori
5LP5_B Chain B, Complex Between Penicillin-binding Protein (pbp2) And
Mrec From Helicobacter Pylori
Length=594
Score = 89.0 bits (219), Expect = 2e-18, Method: Compositional matrix adjust.
Identities = 80/312 (26%), Positives = 125/312 (40%), Gaps = 35/312 (11%)
Frame = +1
Query 385 PKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKD 564
P + ++D ++QK + NK+GA + +D + G +L S P Y+ F GG
Sbjct 225 PSTNNHLQLSLDKRLQKEADKLFENKRGAILVMDAENGELLVAGSYPEYNLNDFVGGISQ 284
Query 565 DEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYIL 744
D+ W L D P+LNR YPPGS K+ + E + + P +I
Sbjct 285 DK--WQKLQDDIYNPLLNRFANALYPPGSVVKMGVGLSFLENLHITENTTIPTPP--FIE 340
Query 745 PGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTI 924
G GN+ + A+ S + F K G E+ +KL + GF ++ +
Sbjct 341 VGKHK--FRDWKKTGHGNSNLYKAIRESVDVYFYKFGLEISIEKLSKTLREVGFGEKTGV 398
Query 925 PIRAD-----------ASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM 1071
+ + F + T + AIGQ ATPLQ+ IA G L
Sbjct 399 DLPNEFVGIVPDNLWKLKRFNQDWRVGDTLITAIGQGSFLATPLQVLAYTGLIA-TGKLA 457
Query 1072 EPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQL---MESVVQHGTGT---NAKIP 1233
P+ + + + L ++ +KLQ L M V H GT + +
Sbjct 458 TPHF-----------AINNKQPLKDPLNSFQKKKLQALRVGMYEVCNHKDGTAYHSTRGS 506
Query 1234 GVTVGGKTGTAQ 1269
+T+ KTGTAQ
Sbjct 507 KITLACKTGTAQ 518
>3EQV_A Chain A, Crystal Structure Of Penicillin-binding Protein 2 From
Neisseria Gonorrhoeae Containing Four Mutations Associated
With Penicillin Resistance
3EQV_B Chain B, Crystal Structure Of Penicillin-binding Protein 2 From
Neisseria Gonorrhoeae Containing Four Mutations Associated
With Penicillin Resistance
Length=542
Score = 87.8 bits (216), Expect = 5e-18, Method: Compositional matrix adjust.
Identities = 117/457 (26%), Positives = 191/457 (42%), Gaps = 81/457 (18%)
Frame = +1
Query 178 QAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQ-LE-KLEDGILSGTDSRLF 351
+A+ +FV + KR + G ++A V G++ + LE LED + + +
Sbjct 113 KALGLENFV--FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVV 170
Query 352 FR----NTLDML----TGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAID 486
R N +D L P+ G D++ ++D ++Q +E L K G V +D
Sbjct 171 LRDRQGNIVDSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLD 230
Query 487 PKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLV 666
+TG IL L +TP+YDP G D E+ NRA+ + PGS K
Sbjct 231 ARTGEILALANTPAYDPNR--PGRADSEQRR------------NRAVTDMIEPGSAIKPF 276
Query 667 TAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCG---NATVQHAMDLSCNT 837
A A + GK + P + I SP D + V+ M S N
Sbjct 277 VIAKALDAGKTDLNERLNTQPYK----------IGPSPVRDTHVYPSLDVRGIMQKSSNV 326
Query 838 VFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGS----------KPDGTAMD 987
K+ A G + E + F E+ I +R S FP + +P A
Sbjct 327 GTSKLSARFGAE------EMYDFYHELGIGVRMH-SGFPGETAGLLRNWRRWRPIEQATM 379
Query 988 AIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATA 1167
+ G + + + LQ+A A+ ++G L+ P ++ A Q + TA
Sbjct 380 SFG-YGLQLSLLQLARAYTALTHDGVLL-PLSFEKQAVA---------PQGKRIFKEXTA 428
Query 1168 QKLQQLMESVVQ-HGTGTNAKIPGVTVGGKTGTAQ---HGQDNSGLPFAWFVSYAKGQDG 1335
++++ LM SV + GTGT + G VG KTGTA+ +G+ F+ +A ++
Sbjct 429 REVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGRYVDNKHVGTFIGFAPAKN- 487
Query 1336 KQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
+V VAV +++ + A GG +A K +M +L
Sbjct 488 PRVIVAVTIDEPT--AHGYYGGVVAGSPFKKIMGGSL 522
>3EQU_A Chain A, Crystal Structure Of Penicillin-Binding Protein 2 From
Neisseria Gonorrhoeae
3EQU_B Chain B, Crystal Structure Of Penicillin-Binding Protein 2 From
Neisseria Gonorrhoeae
Length=542
Score = 87.0 bits (214), Expect = 9e-18, Method: Compositional matrix adjust.
Identities = 116/457 (25%), Positives = 190/457 (42%), Gaps = 81/457 (18%)
Frame = +1
Query 178 QAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQ-LE-KLEDGILSGTDSRLF 351
+A+ +FV + KR + G ++A V G++ + LE LED + + +
Sbjct 113 KALGLENFV--FEKELKRHYPXGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVV 170
Query 352 FR----NTLDML----TGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAID 486
R N +D L P+ G D++ ++D ++Q +E L K G V +D
Sbjct 171 LRDRQGNIVDSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLD 230
Query 487 PKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLV 666
+TG IL L +TP+YDP G D E+ NRA+ + PGS K
Sbjct 231 ARTGEILALANTPAYDPNR--PGRADSEQ------------RRNRAVTDXIEPGSAIKPF 276
Query 667 TAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCG---NATVQHAMDLSCNT 837
A A + GK + P + I SP D + V+ S N
Sbjct 277 VIAKALDAGKTDLNERLNTQPYK----------IGPSPVRDTHVYPSLDVRGIXQKSSNV 326
Query 838 VFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGS----------KPDGTAMD 987
K+ A G + E + F E+ I +R S FP + +P A
Sbjct 327 GTSKLSARFGAE------EXYDFYHELGIGVRXH-SGFPGETAGLLRNWRRWRPIEQATX 379
Query 988 AIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATA 1167
+ G + + + LQ+A A+ ++G L+ P ++ A Q + TA
Sbjct 380 SFG-YGLQLSLLQLARAYTALTHDGVLL-PLSFEKQAVA---------PQGKRIFKEXTA 428
Query 1168 QKLQQLMESVVQ-HGTGTNAKIPGVTVGGKTGTAQ---HGQDNSGLPFAWFVSYAKGQDG 1335
++++ L SV + GTGT + G VG KTGTA+ +G+ A F+ +A ++
Sbjct 429 REVRNLXVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHVATFIGFAPAKN- 487
Query 1336 KQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
+V VAV +++ + A GG +A P K + +L
Sbjct 488 PRVIVAVTIDEPT--AHGYYGGVVAGPPFKKIXGGSL 522
>5KSH_A Chain A, Crystal Structure Of Penicillin-binding Protein 2 From
Neisseria Gonorrhoeae Containing An A501t Mutation Associated
With Cephalosporin Resistance
5KSH_B Chain B, Crystal Structure Of Penicillin-binding Protein 2 From
Neisseria Gonorrhoeae Containing An A501t Mutation Associated
With Cephalosporin Resistance
Length=540
Score = 85.9 bits (211), Expect = 2e-17, Method: Compositional matrix adjust.
Identities = 116/457 (25%), Positives = 190/457 (42%), Gaps = 81/457 (18%)
Frame = +1
Query 178 QAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQ-LE-KLEDGILSGTDSRLF 351
+A+ +FV + KR + G ++A V G++ + LE LED + + +
Sbjct 111 KALGLENFV--FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVV 168
Query 352 FR----NTLDML----TGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAID 486
R N +D L P+ G D++ ++D ++Q +E L K G V +D
Sbjct 169 LRDRQGNIVDSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLD 228
Query 487 PKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLV 666
+TG IL L +TP+YDP G D E+ NRA+ + PGS K
Sbjct 229 ARTGEILALANTPAYDPNR--PGRADSEQRR------------NRAVTDMIEPGSAIKPF 274
Query 667 TAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCG---NATVQHAMDLSCNT 837
A A + GK + P + I SP D + V+ M S N
Sbjct 275 VIAKALDAGKTDLNERLNTQPYK----------IGPSPVRDTHVYPSLDVRGIMQKSSNV 324
Query 838 VFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGS----------KPDGTAMD 987
K+ A G + E + F E+ I +R S FP + +P A
Sbjct 325 GTSKLSARFGAE------EMYDFYHELGIGVRMH-SGFPGETAGLLRNWRRWRPIEQATM 377
Query 988 AIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATA 1167
+ G + + + LQ+A A+ ++G L+ P ++ A Q + TA
Sbjct 378 SFG-YGLQLSLLQLARAYTALTHDGVLL-PLSFEKQAVA---------PQGKRIFKEXTA 426
Query 1168 QKLQQLMESVVQ-HGTGTNAKIPGVTVGGKTGTAQ---HGQDNSGLPFAWFVSYAKGQDG 1335
++++ LM SV + GTGT + G VG KTGT + +G+ F+ +A ++
Sbjct 427 REVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTTRKLVNGRYVDNKHVGTFIGFAPAKN- 485
Query 1336 KQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
+V VAV +++ + A GG +A K +M +L
Sbjct 486 PRVIVAVTIDEPT--AHGYYGGVVAGSPFKKIMGGSL 520
>4L0L_A Chain A, Crystal Structure Of P.aeruginosa Pbp3 In Complex With
Compound 4
Length=537
Score = 78.2 bits (191), Expect = 5e-15, Method: Compositional matrix adjust.
Identities = 96/382 (25%), Positives = 170/382 (45%), Gaps = 64/382 (17%)
Frame = +1
Query 373 LTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSY 531
+T K G + +ID ++Q L N K G+ V +D KTG IL + + P+Y
Sbjct 167 VTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTY 226
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
+P ++ + A M NRA+ + + PGST K + + A +G+++ PS
Sbjct 227 NPNN----RRNLQPA----------AMRNRAMIDVFEPGSTVKPFSMSAALASGRWK-PS 271
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDL------SCNTVFGKMGAELGGD 873
D+ D + PGT I D + Q +DL S N K+ ++G +
Sbjct 272 DIVD-----VYPGTLQ--IGRYTIRDVSRNSRQ--LDLTGILIKSSNVGISKIAFDIGAE 322
Query 874 KLRAQAEKFGFNKEVTIPIRAD-ASHFPSGSK-PDG-TAMDAIGQHDTRATPLQMAMVAA 1044
+ + ++ G ++ + + + P+ K P TA A G + T +Q+A A
Sbjct 323 SIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYG-YGLSVTAIQLAHAYA 381
Query 1045 AIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-N 1221
A+AN+G + + + V Q +SP A +Q +++ VV+ G
Sbjct 382 ALANDGKSVPLSMTRVDRVPDGV----------QVISPEVASTVQGMLQQVVEAQGGVFR 431
Query 1222 AKIPGVTVGGKTGTAQH-------GQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSE 1380
A++PG GK+GTA+ ++N+ + F +A D ++A+ VV+++ S
Sbjct 432 AQVPGYHAAGKSGTARKVSVGTKGYRENAYR--SLFAGFAPATD-PRIAMVVVIDEPSKA 488
Query 1381 AQQISGGSLAAPIAKAMMQAAL 1446
GG ++AP+ +M AL
Sbjct 489 G--YFGGLVSAPVFSKVMAGAL 508
>3PBN_A Chain A, Crystal Structure Of Apo Pbp3 From Pseudomonas Aeruginosa
3PBO_A Chain A, Crystal Structure Of Pbp3 Complexed With Ceftazidime
3PBQ_A Chain A, Crystal Structure Of Pbp3 Complexed With Imipenem
3PBR_A Chain A, Crystal Structure Of Pbp3 Complexed With Meropenem
3PBS_A Chain A, Crystal Structure Of Pbp3 Complexed With Aztreonam
3PBT_A Chain A, Crystal Structure Of Pbp3 Complexed With Mc-1
4OOL_A Chain A, Crystal Structure Of Pbp3 In Complex With Compound 14
((2e)-2-({[(2s)- 2-{[(2z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(1,5-dihydroxy-4-oxo-1,4-
Dihydropyridin-2-yl)methoxy]imino}acetyl]amino}-3-
Oxopropyl]oxy}imino)pentanedioic Acid)
4OOM_A Chain A, Crystal Structure Of Pbp3 In Complex With Bal30072 ((2z)-2-(2-amino-1,
3-thiazol-4-yl)-2-{[(1,5-dihydroxy-4-oxo-1,4-dihydropyridin-2-yl)
Methoxy]imino}-n-{(2s)-1-hydroxy-3-methyl-3-[(sulfooxy)amino]butan-2-
Yl}ethanamide)
4WEJ_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa Pbp3 With
A R4 Substituted Allyl Monocarbam
4WEL_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa Pbp3 With
Smc-3176
Length=538
Score = 77.8 bits (190), Expect = 7e-15, Method: Compositional matrix adjust.
Identities = 96/382 (25%), Positives = 170/382 (45%), Gaps = 64/382 (17%)
Frame = +1
Query 373 LTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSY 531
+T K G + +ID ++Q L N K G+ V +D KTG IL + + P+Y
Sbjct 168 VTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTY 227
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
+P ++ + A M NRA+ + + PGST K + + A +G+++ PS
Sbjct 228 NPNN----RRNLQPA----------AMRNRAMIDVFEPGSTVKPFSMSAALASGRWK-PS 272
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDL------SCNTVFGKMGAELGGD 873
D+ D + PGT I D + Q +DL S N K+ ++G +
Sbjct 273 DIVD-----VYPGTLQ--IGRYTIRDVSRNSRQ--LDLTGILIKSSNVGISKIAFDIGAE 323
Query 874 KLRAQAEKFGFNKEVTIPIRAD-ASHFPSGSK-PDG-TAMDAIGQHDTRATPLQMAMVAA 1044
+ + ++ G ++ + + + P+ K P TA A G + T +Q+A A
Sbjct 324 SIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYG-YGLSVTAIQLAHAYA 382
Query 1045 AIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-N 1221
A+AN+G + + + V Q +SP A +Q +++ VV+ G
Sbjct 383 ALANDGKSVPLSMTRVDRVPDGV----------QVISPEVASTVQGMLQQVVEAQGGVFR 432
Query 1222 AKIPGVTVGGKTGTAQH-------GQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSE 1380
A++PG GK+GTA+ ++N+ + F +A D ++A+ VV+++ S
Sbjct 433 AQVPGYHAAGKSGTARKVSVGTKGYRENAYR--SLFAGFAPATD-PRIAMVVVIDEPSKA 489
Query 1381 AQQISGGSLAAPIAKAMMQAAL 1446
GG ++AP+ +M AL
Sbjct 490 G--YFGGLVSAPVFSKVMAGAL 509
>4BJP_A Chain A, Crystal Structure Of E. Coli Penicillin Binding Protein
3
Length=511
Score = 77.8 bits (190), Expect = 7e-15, Method: Compositional matrix adjust.
Identities = 102/440 (23%), Positives = 177/440 (40%), Gaps = 70/440 (16%)
Frame = +1
Query 211 LRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLE---DGILSGTDSRLFFRN------T 363
LR + +R + G + A + G+++ D+ +E +E D L+G R
Sbjct 95 LREESRRYYPSGEVTAHLIGFTN--VDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI 152
Query 364 LDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVST 522
D+ + + ++ +ID ++Q + L N + G+AV +D TG +L + ++
Sbjct 153 EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANS 212
Query 523 PSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQ 702
PSY+P +G P + M NR + + + PGST K + TA + G +
Sbjct 213 PSYNPNNLSG--------------TPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVR 258
Query 703 SPSDVTDTPDQYILPGTSTPLINASPTEDCGN---ATVQHAMDLSCNTVFGKMGAELGGD 873
S + P + IN +D T+ + S N K+ +
Sbjct 259 ENSVLNTIPYR----------INGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSS 308
Query 874 KLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQ------HDTRATPLQMAM 1035
L +FG K + + + SG P I + + TPLQ+A
Sbjct 309 ALVDTYSRFGLGKATNLGLVGER----SGLYPQKQRWSDIERATFSFGYGLMVTPLQLAR 364
Query 1036 VAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESV-VQHGT 1212
V A I + G + P + + E +++ + + +MESV + G
Sbjct 365 VYATIGSYG-IYRPLSITKVDP-----PVPGERVFPESI----VRTVVHMMESVALPGGG 414
Query 1213 GTNAKIPGVTVGGKTGTAQH-GQDNSGLPFAWFVSYAKG-QDGKQVAVAVVVEDGSSEAQ 1386
G A I G + KTGTA+ G D G +++Y G Q A+VV +A
Sbjct 415 GVKAAIKGYRIAIKTGTAKKVGPD--GRYINKYIAYTAGVAPASQPRFALVVVINDPQAG 472
Query 1387 QISGGSLAAPIAKAMMQAAL 1446
+ GG+++AP+ A+M L
Sbjct 473 KYYGGAVSAPVFGAIMGGVL 492
>4FSF_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa Pbp3 Complexed
With Compound 14
Length=536
Score = 77.8 bits (190), Expect = 7e-15, Method: Compositional matrix adjust.
Identities = 96/382 (25%), Positives = 170/382 (45%), Gaps = 64/382 (17%)
Frame = +1
Query 373 LTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSY 531
+T K G + +ID ++Q L N K G+ V +D KTG IL + + P+Y
Sbjct 166 VTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTY 225
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
+P ++ + A M NRA+ + + PGST K + + A +G+++ PS
Sbjct 226 NPNN----RRNLQPA----------AMRNRAMIDVFEPGSTVKPFSMSAALASGRWK-PS 270
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDL------SCNTVFGKMGAELGGD 873
D+ D + PGT I D + Q +DL S N K+ ++G +
Sbjct 271 DIVD-----VYPGTLQ--IGRYTIRDVSRNSRQ--LDLTGILIKSSNVGISKIAFDIGAE 321
Query 874 KLRAQAEKFGFNKEVTIPIRAD-ASHFPSGSK-PDG-TAMDAIGQHDTRATPLQMAMVAA 1044
+ + ++ G ++ + + + P+ K P TA A G + T +Q+A A
Sbjct 322 SIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYG-YGLSVTAIQLAHAYA 380
Query 1045 AIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-N 1221
A+AN+G + + + V Q +SP A +Q +++ VV+ G
Sbjct 381 ALANDGKSVPLSMTRVDRVPDGV----------QVISPEVASTVQGMLQQVVEAQGGVFR 430
Query 1222 AKIPGVTVGGKTGTAQH-------GQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSE 1380
A++PG GK+GTA+ ++N+ + F +A D ++A+ VV+++ S
Sbjct 431 AQVPGYHAAGKSGTARKVSVGTKGYRENAYR--SLFAGFAPATD-PRIAMVVVIDEPSKA 487
Query 1381 AQQISGGSLAAPIAKAMMQAAL 1446
GG ++AP+ +M AL
Sbjct 488 G--YFGGLVSAPVFSKVMAGAL 507
>4WEK_A Chain A, Crystal Structure Of Pseudomonas Aeruginosa Pbp3 With
A R4 Substituted Vinyl Monocarbam
Length=538
Score = 77.8 bits (190), Expect = 7e-15, Method: Compositional matrix adjust.
Identities = 96/382 (25%), Positives = 170/382 (45%), Gaps = 64/382 (17%)
Frame = +1
Query 373 LTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSY 531
+T K G + +ID ++Q L N K G+ V +D KTG IL + + P+Y
Sbjct 168 VTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTY 227
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
+P ++ + A M NRA+ + + PGST K + + A +G+++ PS
Sbjct 228 NPNN----RRNLQPA----------AMRNRAMIDVFEPGSTVKPFSMSAALASGRWK-PS 272
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDL------SCNTVFGKMGAELGGD 873
D+ D + PGT I D + Q +DL S N K+ ++G +
Sbjct 273 DIVD-----VYPGTLQ--IGRYTIRDVSRNSRQ--LDLTGILIKSSNVGISKIAFDIGAE 323
Query 874 KLRAQAEKFGFNKEVTIPIRAD-ASHFPSGSK-PDG-TAMDAIGQHDTRATPLQMAMVAA 1044
+ + ++ G ++ + + + P+ K P TA A G + T +Q+A A
Sbjct 324 SIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYG-YGLSVTAIQLAHAYA 382
Query 1045 AIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-N 1221
A+AN+G + + + V Q +SP A +Q +++ VV+ G
Sbjct 383 ALANDGKSVPLSMTRVDRVPDGV----------QVISPEVASTVQGMLQQVVEAQGGVFR 432
Query 1222 AKIPGVTVGGKTGTAQH-------GQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSE 1380
A++PG GK+GTA+ ++N+ + F +A D ++A+ VV+++ S
Sbjct 433 AQVPGYHAAGKSGTARKVSVGTKGYRENAYR--SLFAGFAPATD-PRIAMVVVIDEPSKA 489
Query 1381 AQQISGGSLAAPIAKAMMQAAL 1446
GG ++AP+ +M AL
Sbjct 490 G--YFGGLVSAPVFSKVMAGAL 509
>3OC2_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa
3OCL_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Carbenicillin
3OCN_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Ceftazidime
4KQO_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Piperacillin
4KQO_B Chain B, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Piperacillin
4KQQ_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With (5s)-penicilloic Acid
4KQQ_B Chain B, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With (5s)-penicilloic Acid
4KQR_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With (5s)-penicilloic Acid
4KQR_B Chain B, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With (5s)-penicilloic Acid
5DF7_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Azlocillin
5DF7_B Chain B, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Azlocillin
5DF8_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Cefoperazone
5DF8_B Chain B, Crystal Structure Of Penicillin-binding Protein 3 From
Pseudomonas Aeruginosa In Complex With Cefoperazone
5DF9_A Chain A, Crystal Structure Of Penicillin-binding Protein 3 In
Complex With Deacylated Product Of Cefoperazone
Length=564
Score = 77.8 bits (190), Expect = 9e-15, Method: Compositional matrix adjust.
Identities = 96/382 (25%), Positives = 170/382 (45%), Gaps = 64/382 (17%)
Frame = +1
Query 373 LTGKPKQGGDVVTTIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSY 531
+T K G + +ID ++Q L N K G+ V +D KTG IL + + P+Y
Sbjct 194 VTKNAKPGKTLALSIDLRLQYLAHRELRNALLENGAKAGSLVIMDVKTGEILAMTNQPTY 253
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
+P ++ + A M NRA+ + + PGST K + + A +G+++ PS
Sbjct 254 NPNN----RRNLQPA----------AMRNRAMIDVFEPGSTVKPFSMSAALASGRWK-PS 298
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDL------SCNTVFGKMGAELGGD 873
D+ D + PGT I D + Q +DL S N K+ ++G +
Sbjct 299 DIVD-----VYPGTLQ--IGRYTIRDVSRNSRQ--LDLTGILIKSSNVGISKIAFDIGAE 349
Query 874 KLRAQAEKFGFNKEVTIPIRAD-ASHFPSGSK-PDG-TAMDAIGQHDTRATPLQMAMVAA 1044
+ + ++ G ++ + + + P+ K P TA A G + T +Q+A A
Sbjct 350 SIYSVMQQVGLGQDTGLGFPGERVGNLPNHRKWPKAETATLAYG-YGLSVTAIQLAHAYA 408
Query 1045 AIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-N 1221
A+AN+G + + + V Q +SP A +Q +++ VV+ G
Sbjct 409 ALANDGKSVPLSMTRVDRVPDGV----------QVISPEVASTVQGMLQQVVEAQGGVFR 458
Query 1222 AKIPGVTVGGKTGTAQH-------GQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSE 1380
A++PG GK+GTA+ ++N+ + F +A D ++A+ VV+++ S
Sbjct 459 AQVPGYHAAGKSGTARKVSVGTKGYRENAYR--SLFAGFAPATD-PRIAMVVVIDEPSKA 515
Query 1381 AQQISGGSLAAPIAKAMMQAAL 1446
GG ++AP+ +M AL
Sbjct 516 G--YFGGLVSAPVFSKVMAGAL 535
>4ZTK_A Chain A, Transpeptidase Domain Of Ftsi4 D,d-transpeptidase From
Legionella Pneumophila.
Length=316
Score = 64.3 bits (155), Expect = 6e-11, Method: Compositional matrix adjust.
Identities = 77/295 (26%), Positives = 126/295 (43%), Gaps = 53/295 (18%)
Frame = +1
Query 589 DSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLI 768
+S NK + + R + PGST K AA A +G S + D Y + G +
Sbjct 61 ESSKNKGLESWKSR-IFSPGSTIKPFIAAAAINSGN-SSETKNYDCHSPYYIAGKTFTNY 118
Query 769 NASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASH 948
N+ + +A++ A+ S N ++ E G +R + +FGF D +
Sbjct 119 NS----NVESASLADAIAKSINVCLIRVSQEAGVPVIRKKLTEFGF----------DXNS 164
Query 949 FPSGSKPDGT--AMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY-LVAQEGSA---GN 1110
+ + D A A+G++ P+ + SL++ Y ++A +G + GN
Sbjct 165 WWQADQSDDLQLAXAALGEN----IPVTIE----------SLIKSYAILANKGHSFDRGN 210
Query 1111 VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSG 1290
S +S + + +E+ V +GTG A IPGV+V GKTGT D
Sbjct 211 ----------SAIISETSTNSINHXLENAVTNGTGKLAVIPGVSVAGKTGTVIENNDKYL 260
Query 1291 LPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQ---QISGGSLAAPIAKAMMQAAL 1446
FA +V D + + VV+E+G +SGG LAAP+ + + AL
Sbjct 261 ALFAGYVP----ADNPRYVLLVVIEEGYFSKNGKTLVSGGELAAPVFRNVAXDAL 311
>4OVD_A Chain A, Crystal structure of a putative peptidoglycan glycosyltransferase
from Atopobium parvulum DSM 20469
Length=564
Score = 65.5 bits (158), Expect = 7e-11, Method: Compositional matrix adjust.
Identities = 102/396 (26%), Positives = 160/396 (40%), Gaps = 71/396 (18%)
Frame = +1
Query 199 FVNGLRYKYKRSWVDGPMYAPVTGY-SSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
FVN KR + G + + G+ ++ AS LE + IL+GT+
Sbjct 146 FVNNT----KRVYPYGNVGVQILGFVNADNEGASGLEYYYNDILAGTNGHXIVETGAG-- 199
Query 376 TGKPKQGG-----------DVVTTIDPKVQKAGFEGL--------GNKKGAAVAIDPKTG 498
G P GG D+V +ID ++QK E L + G+ A +P+TG
Sbjct 200 -GTPIAGGTSNITEAQNGQDIVLSIDIELQKKAEEQLTAAVKDFEAKQGGSIXAXNPRTG 258
Query 499 AILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLN-RALRETYPPGSTFKLVTAA 675
I S P D + D+ + LN + + Y PGS FK++T +
Sbjct 259 EIYAAASYPLPDFES---------------DNGVDYESLNLKLVSSIYEPGSVFKVITTS 303
Query 676 TAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMG 855
+ + P+ + P + L + + + +V+ + S N +
Sbjct 304 IGVDNNLF-GPNSTFNIPTE--LQVGDNKVTDDDWRTSAXDXSVREXLRRSSNVGXAFLE 360
Query 856 AELGGDKLRAQA-EKFG--------FNKEVTIPIRA-DASHFPSGSKPDGTAMDAIGQHD 1005
+L GDK A+ +KFG F E T +R+ D P+G A GQ
Sbjct 361 TQLIGDKRFAEGIDKFGIGHTTGIDFPGEATGIVRSLDQYEGPTGGNX------AFGQG- 413
Query 1006 TRATP-LQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQ 1182
A P +Q+ A+AN G+ + P+ +G G +S T+ +S +TA
Sbjct 414 -LAIPFIQVIRAYTAVANKGTXVTPHFXVSKG--GQEVSWPTK----DVISSSTASAELD 466
Query 1183 LMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSG 1290
+VV+ GTG A I G TV GKTGT Q + +G
Sbjct 467 XXTTVVKEGTGVRAGIYGYTVAGKTGTGQQVVEETG 502
>4U3T_A Chain A, Crystal Structure Of The Transpeptidase Domain Of Neisseria
Gonorrhoeae Penicillin-binding Protein 2 Derived From
The Penicillin- Resistant Strain 6140
4U3T_B Chain B, Crystal Structure Of The Transpeptidase Domain Of Neisseria
Gonorrhoeae Penicillin-binding Protein 2 Derived From
The Penicillin- Resistant Strain 6140
Length=330
Score = 60.1 bits (144), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 91/374 (24%), Positives = 146/374 (39%), Gaps = 92/374 (25%)
Frame = +1
Query 412 TIDPKVQKAGFEGLGN-------KKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDE 570
++D ++Q +E L K G V +D +TG IL L +TP
Sbjct 7 SLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEILALANTPGR------------- 53
Query 571 KAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPG 750
NRA+ + PGS K A A + GK + P +
Sbjct 54 ---------------NRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYK----- 93
Query 751 TSTPLINASPTEDCGNAT----VQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEV 918
I SP D + V+ M S N K+ A G + E + F E+
Sbjct 94 -----IGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAE------EMYDFYHEL 142
Query 919 TIPIRADASHFPSGS----------KPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSL 1068
I +R S FP + +P A + G + + + LQ+A A+ ++G L
Sbjct 143 GIGVRMH-SGFPGETAGLLRNWRRWRPIEQATMSFG-YGLQLSLLQLARAYTALTHDGVL 200
Query 1069 M----EPYLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQ-HGTGTNAKIP 1233
+ E VA +G + +TA++++ LM SV + GTGT +
Sbjct 201 LPLSFEKQAVAPQGK--------------RIFKESTAREVRNLMVSVTEPGGTGTAGAVD 246
Query 1234 GVTVGGKTGTAQ---HGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGS 1404
G VG KTGTA+ +G+ F+ +A ++ +V VAV +++ + A GG
Sbjct 247 GFDVGAKTGTARKLVNGRYVDNKHVGTFIGFAPAKN-PRVIVAVTIDEPT--AHGYYGGV 303
Query 1405 LAAPIAKAMMQAAL 1446
+A K +M +L
Sbjct 304 VAGSPFKKIMGGSL 317
>3ZG8_B Chain B, Crystal Structure Of Penicillin Binding Protein 4 From
Listeria Monocytogenes In The Ampicillin Bound Form
3ZGA_B Chain B, Crystal Structure Of Peniscillin-binding Protein 4 From
Listeria Monocytogenes In The Carbenicillin Bound Form
3ZG9_B Chain B, Crystal Structure Of Penicillin-binding Protein 4 From
Listeria Monocytogenes In The Cefuroxime Bound Form
Length=537
Score = 60.5 bits (145), Expect = 2e-09, Method: Compositional matrix adjust.
Identities = 79/285 (28%), Positives = 116/285 (41%), Gaps = 47/285 (16%)
Frame = +1
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
AV +DP TG I LV F G NRA + PGS
Sbjct 180 AVLMDPATGGIRALVG--GRGEHVFRG--------------------FNRATQMKAQPGS 217
Query 652 TFK-LVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLS 828
T K L A ++G Y S + D Y G TP N G + A+ S
Sbjct 218 TMKPLAVYTPALQSG-YDVDSMLKDEKITY--KGNYTP-TNVGGVY-SGEVPMYKAVANS 272
Query 829 CNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDT 1008
N + ++G DK EKFG +T+P + D T A+G
Sbjct 273 INAPAVWLLDQIGIDKGVKSVEKFG----ITVP------------EKDRTLGLALGGMSK 316
Query 1009 RATPLQMAMVAAAIANNGSLMEPYLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQL 1185
A+P++MA A ANNG+ E +++ + +GN + + + Q +S + ++ +
Sbjct 317 GASPVEMATAYATFANNGAKPESHIITKIVDPSGNTVYENVP-KTKQIISETVSNEMTSM 375
Query 1186 MESVVQHGTGTNAKIPGVTVGGKTGTAQ-HGQDNSGLPFAWFVSY 1317
+ V+ GTG +A + G + GKTG+ Q D SG WFV Y
Sbjct 376 LLDVINTGTGQSAAVSGHEMAGKTGSTQVPFDDTSGTKDQWFVGY 420
>2WAF_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From Streptococcus
Pneumoniae (strain R6)
Length=682
Score = 58.2 bits (139), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 94/333 (28%), Positives = 140/333 (42%), Gaps = 64/333 (19%)
Frame = +1
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
AVA++PKTGA+L + G K D K L D + N + PGS
Sbjct 345 AVALNPKTGAVLSM------------SGIKHDLKTG-ELTPDSLGTVTN-----VFVPGS 386
Query 652 TFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCGN--ATVQHAMDL 825
K T ++ +E G +TD Q I+ S P IN+ T+ G+ T A++
Sbjct 387 VVKAATISSGWENGVLSGNQTLTD---QSIVFQGSAP-INSWYTQAYGSFPITAVQALEY 442
Query 826 SCNT-----VFGKMG-------------AELGGDKLRAQAEKFGFNKEVTIPIRADASHF 951
S NT G MG E +KLR+ ++G I + +++ F
Sbjct 443 SSNTYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGF 502
Query 952 -PSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVA------QEGSAGN 1110
P +A GQ D TP+Q+A A IANNG + P +V +G G+
Sbjct 503 VPKEYSFANYITNAFGQFDNY-TPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD 561
Query 1111 VISTHTEHQLSQA-VSPATAQKLQQLMESVVQHGTG--------TNAKIPGVTVGGKTGT 1263
+I ++++ +S + L Q V HGT +N + V++ GKTGT
Sbjct 562 LIQQLQPTEMNKVNISDSDMSILHQGFYQVA-HGTSGLTTGRAFSNGAL--VSISGKTGT 618
Query 1264 AQ-HGQDNSGLPFAWFVSYAKGQDGKQVAVAVV 1359
A+ + D V+YA D Q+AVAVV
Sbjct 619 AESYVADGQQATNTNAVAYAPS-DNPQIAVAVV 650
>2V2F_F Chain F, Crystal Structure Of Pbp1a From Drug-resistant Strain
5204 From Streptococcus Pneumoniae
Length=390
Score = 57.8 bits (138), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 71/251 (28%), Positives = 111/251 (44%), Gaps = 35/251 (14%)
Frame = +1
Query 616 NRALRETYPPGSTFKLVT-AATAFETGKYQSPSD-VTDTPDQYILPGTSTPLINASPTED 789
N+A+ GS K +T A A E G Y S + V D P Y PGTSTP+ N
Sbjct 96 NQAVETNRDWGSAMKPITDYAPAIEYGVYDSTATMVNDIPYNY--PGTSTPVYNWDRAY- 152
Query 790 CGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKP 969
GN T+Q+A+ S N + ++G D+ + G + A +S+ +K
Sbjct 153 FGNITLQYALQQSRNVTAVETLNKVGLDRAKTFLNGLGIDYPSMHYANAISSNTTESNKQ 212
Query 970 DGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLS-- 1143
G A+ +MA AA AN G +P + + V S ++ + S
Sbjct 213 YG------------ASSEKMAAAYAAFANGGIYHKPMYINKV-----VFSDGSKKEFSDV 255
Query 1144 --QAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQD-------NSGL- 1293
+A+ TA + ++M++V+ +GTG A +P + GKTGT+ + D N+G
Sbjct 256 GTRAMKETTAYMMTEMMKTVLAYGTGRGAYLPWLAQAGKTGTSNYTDDEIEKHIKNTGYV 315
Query 1294 -PFAWFVSYAK 1323
P FV Y +
Sbjct 316 APDEMFVGYTR 326
>2C5W_B Chain B, Penicillin-Binding Protein 1a (Pbp-1a) Acyl-Enzyme Complex
(Cefotaxime) From Streptococcus Pneumoniae
Length=385
Score = 55.8 bits (133), Expect = 6e-08, Method: Compositional matrix adjust.
Identities = 63/226 (28%), Positives = 105/226 (46%), Gaps = 26/226 (12%)
Frame = +1
Query 613 LNRALRETYPPGSTFKLVT-AATAFETGKYQSPSDVT-DTPDQYILPGTSTPLINASPTE 786
+N+A+ GST K +T A A E G Y+S + + D P Y PGT+TP+ N
Sbjct 93 INQAVETNRDWGSTMKPITDYAPALEYGVYESTATIVHDEPYNY--PGTNTPVYNWDRGY 150
Query 787 DCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSK 966
GN T+Q+A+ S N + ++G ++ + G I + H+ +
Sbjct 151 -FGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLNGLG--------IDYPSIHYSNAIS 201
Query 967 PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLS- 1143
+ T D A+ +MA AA AN G+ +P + + V S +E + S
Sbjct 202 SNTTESDK----KYGASSEKMAAAYAAFANGGTYYKPMYIHKV-----VFSDGSEKEFSN 252
Query 1144 ---QAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQH 1272
+A+ TA + +M++V+ +GTG NA + + GKTGT+ +
Sbjct 253 VGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNY 298
>2ZC5_B Chain B, Penicillin-Binding Protein 1a (Pbp 1a) Acyl-Enzyme Complex
(Biapenem) From Streptococcus Pneumoniae
2ZC5_D Chain D, Penicillin-Binding Protein 1a (Pbp 1a) Acyl-Enzyme Complex
(Biapenem) From Streptococcus Pneumoniae
2ZC6_B Chain B, Penicillin-binding Protein 1a (pbp 1a) Acyl-enzyme Complex
(tebipenem) From Streptococcus Pneumoniae
2ZC6_D Chain D, Penicillin-binding Protein 1a (pbp 1a) Acyl-enzyme Complex
(tebipenem) From Streptococcus Pneumoniae
Length=390
Score = 55.1 bits (131), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 63/226 (28%), Positives = 104/226 (46%), Gaps = 26/226 (12%)
Frame = +1
Query 613 LNRALRETYPPGSTFKLVT-AATAFETGKYQSPSDVT-DTPDQYILPGTSTPLINASPTE 786
+N+A+ GST K +T A A E G Y S + + D P Y PGT+TP+ N
Sbjct 95 INQAVETNRDWGSTMKPITDYAPALEYGVYDSTATIVHDEPYNY--PGTNTPVYNWDRGY 152
Query 787 DCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSK 966
GN T+Q+A+ S N + ++G ++ + G I + H+ +
Sbjct 153 -FGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLNGLG--------IDYPSIHYSNAIS 203
Query 967 PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLS- 1143
+ T D A+ +MA AA AN G+ +P + + V S +E + S
Sbjct 204 SNTTESDK----KYGASSEKMAAAYAAFANGGTYYKPMYIHKV-----VFSDGSEKEFSN 254
Query 1144 ---QAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQH 1272
+A+ TA + +M++V+ +GTG NA + + GKTGT+ +
Sbjct 255 VGTRAMKETTAYMMTDMMKTVLSYGTGQNAYLAWLPQAGKTGTSNY 300
>2C6W_B Chain B, Penicillin-Binding Protein 1a (Pbp-1a) From Streptococcus
Pneumoniae
Length=384
Score = 54.7 bits (130), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 63/226 (28%), Positives = 104/226 (46%), Gaps = 26/226 (12%)
Frame = +1
Query 613 LNRALRETYPPGSTFKLVT-AATAFETGKYQSPSDVT-DTPDQYILPGTSTPLINASPTE 786
+N+A+ GST K +T A A E G Y S + + D P Y PGT+TP+ N
Sbjct 92 INQAVETNRDWGSTMKPITDYAPALEYGVYDSTATIVHDEPYNY--PGTNTPVYNWDRGY 149
Query 787 DCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSK 966
GN T+Q+A+ S N + ++G ++ + G I + H+ +
Sbjct 150 -FGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLNGLG--------IDYPSIHYSNAIS 200
Query 967 PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTHTEHQLS- 1143
+ T D A+ +MA AA AN G+ +P + + V S +E + S
Sbjct 201 SNTTESDK----KYGASSEKMAAAYAAFANGGTYYKPMYIHKV-----VFSDGSEKEFSN 251
Query 1144 ---QAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQH 1272
+A+ TA + +M++V+ +GTG NA + + GKTGT+ +
Sbjct 252 VGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNY 297
>3ZG7_B Chain B, Crystal Structure Of Penicillin-binding Protein 4 From
Listeria Monocytogenes In The Apo Form
Length=537
Score = 53.9 bits (128), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 67/238 (28%), Positives = 99/238 (42%), Gaps = 25/238 (11%)
Frame = +1
Query 613 LNRALRETYPPGSTFK-LVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTED 789
NRA + PGST K L A ++G Y S + D Y G TP N
Sbjct 205 FNRATQXKAQPGSTXKPLAVYTPALQSG-YDVDSXLKDEKITY--KGNYTP-TNVGGVY- 259
Query 790 CGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPSGSKP 969
G A+ S N + ++G DK EKFG +T+P +
Sbjct 260 SGEVPXYKAVANSINAPAVWLLDQIGIDKGVKSVEKFG----ITVP------------EK 303
Query 970 DGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ-EGSAGNVISTHTEHQLSQ 1146
D T A+G A+P++ A A ANNG+ E +++ + +GN + + + Q
Sbjct 304 DRTLGLALGGXSKGASPVEXATAYATFANNGAKPESHIITKIVDPSGNTVYENVP-KTKQ 362
Query 1147 AVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ-HGQDNSGLPFAWFVSY 1317
+S + + + V+ GTG +A + G GKTG+ Q D SG WFV Y
Sbjct 363 IISETVSNEXTSXLLDVINTGTGQSAAVSGHEXAGKTGSTQVPFDDTSGTKDQWFVGY 420
>2WAD_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From Streptococcus
Pneumoniae (strain 5204)
2WAD_B Chain B, Penicillin-binding Protein 2b (pbp-2b) From Streptococcus
Pneumoniae (strain 5204)
2WAD_C Chain C, Penicillin-binding Protein 2b (pbp-2b) From Streptococcus
Pneumoniae (strain 5204)
Length=680
Score = 53.5 bits (127), Expect = 4e-07, Method: Compositional matrix adjust.
Identities = 88/330 (27%), Positives = 133/330 (40%), Gaps = 58/330 (18%)
Frame = +1
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
AVA++P+TGA+L + G K D K L D + N + PGS
Sbjct 345 AVALNPQTGAVLSM------------SGLKHDLKTG-ELTPDSLGTVTN-----VFVPGS 386
Query 652 TFKLVTAATAFETGKYQSPSDVTDTP----------DQYILPGTSTPLINASPTEDCGNA 801
K T ++ +E G +TD P Y L S P+ E NA
Sbjct 387 VVKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSNA 446
Query 802 -TVQHAMDL-----SCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHF-PSG 960
VQ A+ + N G E KLR+ ++G I + +++ P
Sbjct 447 YVVQTALGIMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGLVPKE 506
Query 961 SKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVA------QEGSAGNVIST 1122
+A GQ D TP+Q+A A IANNG + P++V +G G +I
Sbjct 507 YNFANFITNAFGQFDNY-TPMQLAQYVATIANNGVRLAPHIVEGIYDNNDKGGLGELIQA 565
Query 1123 HTEHQLSQA-VSPATAQKLQQLMESVVQHGTG--TNAKI----PGVTVGGKTGTAQH--- 1272
++++ +S + L Q V HGT T + V++ GKTGT +
Sbjct 566 IDTKEINKVNISESDMAILHQGFYQ-VSHGTSPLTTGRAFSDGATVSISGKTGTGESYVA 624
Query 1273 -GQDNSGLPFAWFVSYAKGQDGKQVAVAVV 1359
GQ+ + V+YA ++ Q+AVAVV
Sbjct 625 GGQEATN---TNAVAYAPTEN-PQIAVAVV 650
>2WAE_A Chain A, Penicillin-binding Protein 2b (pbp-2b) From Streptococcus
Pneumoniae (strain 5204)
Length=680
Score = 53.1 bits (126), Expect = 6e-07, Method: Compositional matrix adjust.
Identities = 88/330 (27%), Positives = 133/330 (40%), Gaps = 58/330 (18%)
Frame = +1
Query 472 AVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGS 651
AVA++P+TGA+L + G K D K L D + N + PGS
Sbjct 345 AVALNPQTGAVLSM------------SGLKHDLKTG-ELTPDSLGTVTN-----VFVPGS 386
Query 652 TFKLVTAATAFETGKYQSPSDVTDTP----------DQYILPGTSTPLINASPTEDCGNA 801
K T ++ +E G +TD P Y L S P+ E NA
Sbjct 387 VVKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSNA 446
Query 802 -TVQHAMDL-----SCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHF-PSG 960
VQ A+ + N G E KLR+ ++G I + +++ P
Sbjct 447 YVVQTALGIMGQTYQPNMFVGTSNLESAMGKLRSTFGEYGLGSATGIDLPDESTGLVPKE 506
Query 961 SKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVA------QEGSAGNVIST 1122
+A GQ D TP+Q+A A IANNG + P++V +G G +I
Sbjct 507 YNFANFITNAFGQFDNY-TPMQLAQYVATIANNGVRLAPHIVEGIYDNNDKGGLGELIQA 565
Query 1123 HTEHQLSQA-VSPATAQKLQQLMESVVQHGTG--TNAKI----PGVTVGGKTGTAQH--- 1272
++++ +S + L Q V HGT T + V++ GKTGT +
Sbjct 566 IDTKEINKVNISESDMAILHQGFYQ-VSHGTSPLTTGRAFSDGATVSISGKTGTGESYVA 624
Query 1273 -GQDNSGLPFAWFVSYAKGQDGKQVAVAVV 1359
GQ+ + V+YA ++ Q+AVAVV
Sbjct 625 GGQEANN---TNAVAYAPTEN-PQIAVAVV 650
>3UDF_A Chain A, Crystal Structure Of Apo Pbp1a From Acinetobacter Baumannii
3UDF_B Chain B, Crystal Structure Of Apo Pbp1a From Acinetobacter Baumannii
3UDI_A Chain A, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Penicillin G
3UDI_B Chain B, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Penicillin G
3UDX_A Chain A, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Imipenem
3UDX_B Chain B, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Imipenem
3UE0_A Chain A, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Aztreonam
3UE0_B Chain B, Crystal Structure Of Acinetobacter Baumannii Pbp1a In
Complex With Aztreonam
3UE1_A Chain A, Crystal Strucuture Of Acinetobacter Baumanni Pbp1a In
Complex With Mc- 1
3UE1_B Chain B, Crystal Strucuture Of Acinetobacter Baumanni Pbp1a In
Complex With Mc- 1
Length=731
Score = 50.8 bits (120), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 103/421 (24%), Positives = 153/421 (36%), Gaps = 130/421 (31%)
Frame = +1
Query 367 DMLTGKPKQGGDVVTTID-PKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGT 543
D++ +P + + + PKVQ G +AI+P G+I +V ++
Sbjct 365 DIVRLRPNEAKTAWSLVQVPKVQ-----------GQLIAINPNDGSIEAIVGGYNFYQSK 413
Query 544 FAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSP-SDVT 720
F NRAL+ PGST K A A E G +P S V
Sbjct 414 F-----------------------NRALQGWRQPGSTIKPFLYALALERG--MTPYSMVN 448
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
D+P I G TP S G ++ A+ LS NTV ++ +G ++ R F
Sbjct 449 DSP---ITIGKWTP--KNSDGRYLGMIPLRRALYLSRNTVSVRLLQTVGIERTRQLFMDF 503
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
G +E IP I + P+QMA A AN G ++P+
Sbjct 504 GL-QEDQIPRNY-----------------TIALGTPQVLPIQMATGYATFANGGYRVQPH 545
Query 1081 LVAQ-EGSAGNVIS-----------------------THTEHQLSQAVSPATAQK----- 1173
+ + E + G VI T + Q+ + + QK
Sbjct 546 FIQRIEDAYGKVIYEAKPEYACIPCINAPETTDDAQVTTPDDQVVEVTNKELEQKEKTTK 605
Query 1174 ----------------LQQLMES------------VVQHGTGTNA-KIPGVTVGGKTGTA 1266
Q++++S V++HGTG A KI +GGKTGT
Sbjct 606 QLNLKQTDKNNSQYRQAQRILKSSSAYDMANILRDVIEHGTGRAALKIGRSDLGGKTGTT 665
Query 1267 QHGQDNSGLPFAWFVSYAKGQDGKQVAVAVV-VEDGSSEAQQISGGSLAAPIAKAMMQAA 1443
+D AWF G +GK V V V + ++ ++ GG A PI M A
Sbjct 666 NDAKD------AWFA----GFNGKLVTVTWVGFDQPTTLGRREYGGIAALPIWINFMGQA 715
Query 1444 L 1446
L
Sbjct 716 L 716
>1K25_A Chain A, PBP2x from a Highly Penicillin-resistant Streptococcus
pneumoniae Clinical Isolate
1K25_B Chain B, PBP2x from a Highly Penicillin-resistant Streptococcus
pneumoniae Clinical Isolate
1K25_C Chain C, PBP2x from a Highly Penicillin-resistant Streptococcus
pneumoniae Clinical Isolate
1K25_D Chain D, PBP2x from a Highly Penicillin-resistant Streptococcus
pneumoniae Clinical Isolate
Length=685
Score = 49.7 bits (117), Expect = 6e-06, Method: Compositional matrix adjust.
Identities = 88/379 (23%), Positives = 141/379 (37%), Gaps = 83/379 (22%)
Frame = +1
Query 295 SQLEKLEDGILSGTDSRLFFRNT--------LDMLTGKPKQGGDVVTTIDPKVQK----- 435
S +E + IL+GTD + + ++++ + G DV TT+ +Q
Sbjct 152 SGMESSLNSILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ 211
Query 436 --AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
A E + K A + KTG IL P+++ T G T+D W + N
Sbjct 212 MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITED--FVWRDILYQSN-- 267
Query 610 MLNRALRETYPPGSTFKLVTAATA-----FETGKYQSPSDV----TDTPDQYILPGTSTP 762
Y PGS K++T A++ F +G+Y + S++ T D + G +T
Sbjct 268 ---------YEPGSAMKVMTLASSIDNNTFPSGEYFNSSELKIADATTRDWDVNEGLTT- 317
Query 763 LINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADA 942
G T S N + ++G ++F F +P R
Sbjct 318 ---------GGMMTFLQGFAHSSNVGMSLLEQKMGDATWLDYLKRFKFG----VPTRFGL 364
Query 943 SHFPSGSKPD----GTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLV-------- 1086
+ +G P A + GQ T QM AIAN+G ++EP +
Sbjct 365 TDEYAGQLPADNIVSIAQSSFGQ-GISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNN 423
Query 1087 -----AQEGSAGNVI-----STHTEHQLSQAVSPATAQKLQQLMESVVQHGTGT-NAKIP 1233
+Q+ GN + ST H + P L ++ H TG +P
Sbjct 424 QSVRKSQKEIVGNPVSKEAASTTRNHMILVGTDP--------LYGTMYNHYTGKPIITVP 475
Query 1234 GVTVGGKTGTAQHGQDNSG 1290
G V K+GTAQ + +G
Sbjct 476 GQNVAVKSGTAQIADEKNG 494
>5U47_A Chain A, 1.95 Angstrom Resolution Crystal Structure Of Penicillin
Binding Protein 2x From Streptococcus Thermophilus
Length=707
Score = 48.9 bits (115), Expect = 1e-05, Method: Compositional matrix adjust.
Identities = 91/364 (25%), Positives = 141/364 (39%), Gaps = 56/364 (15%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFR-----NTL---DMLTGKPKQGGDVVTTIDPKVQK------- 435
LE+ + +L+GTD + NTL + +T + G ++ TT+ +Q
Sbjct 176 LERSLNSLLTGTDGEAIYEKDKDGNTLLGTETITKEAIDGKNIYTTLSAPLQTFLETQMD 235
Query 436 AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPML 615
E +A ++ KTG IL P+Y+ T G K W +
Sbjct 236 TFMEQTKGINASATVVNAKTGEILATTQRPTYNSDTLEGQAKKGYD-W-----------V 283
Query 616 NRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCG 795
NR Y PGST K++ + A G + + T + I G + + S E
Sbjct 284 NRLYEAQYEPGSTMKVMLLSAAINNGSFN--PNATYSNANGIKVG-DVEINDWSINEGIS 340
Query 796 NA-TVQHAMDLSCNTVFG-KMGAELGGDKLRAQ---AEKFGFNKEVTIPIRADASHFPSG 960
T+ A S ++ G M + GDK+ + KFG IP R SG
Sbjct 341 KGRTMSFAQGFSYSSNVGMTMLEQAMGDKVWSNYLSLYKFG------IPTRFGMVGESSG 394
Query 961 SKPDGT---AMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGN-VISTHT 1128
+ A + GQ T +QM AI+NNG ++EP + Q + T
Sbjct 395 IVSQNSVNIAQSSFGQG-ISVTQVQMLRAFTAISNNGIMLEPQFIKQVADTNKGTVRTAK 453
Query 1129 EHQLSQAVSPATAQKLQQLMESV---VQHGTGTNAK--IPGVTVGG-----KTGTAQHGQ 1278
+ + + VS A + + M SV + GT N P + VG K+GTAQ
Sbjct 454 KEVIGKPVSKQAASETRNYMISVGTDPEFGTLYNKSEGSPIIQVGNNDVTVKSGTAQVPD 513
Query 1279 DNSG 1290
+ +G
Sbjct 514 EKTG 517
>1RP5_A Chain A, Pbp2x From Streptococcus Pneumoniae Strain 5259 With
Reduced Susceptibility To Beta-Lactam Antibiotics
1RP5_B Chain B, Pbp2x From Streptococcus Pneumoniae Strain 5259 With
Reduced Susceptibility To Beta-Lactam Antibiotics
Length=702
Score = 47.4 bits (111), Expect = 4e-05, Method: Compositional matrix adjust.
Identities = 89/374 (24%), Positives = 138/374 (37%), Gaps = 73/374 (20%)
Frame = +1
Query 295 SQLEKLEDGILSGTDSRLFFRNT--------LDMLTGKPKQGGDVVTTIDPKVQK----- 435
S +E + IL+GTD + + + ++ + G DV TTI +Q
Sbjct 169 SGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSPLQSFMETQ 228
Query 436 --AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
A E + K A + KTG IL P++D T G T+D W + N
Sbjct 229 MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITED--FVWRDILYQSN-- 284
Query 610 MLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPL-INASPTE 786
Y PGST K++ A A + + +V ++ + I T +N T
Sbjct 285 ---------YEPGSTMKVMMLAAAIDNNTFPG-GEVFNSSELKIADATIRDWDVNEGLTG 334
Query 787 DCGNATVQ---HAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPS 957
Q H+ ++ + KMG D L +F F +P R + +
Sbjct 335 GRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLN----RFKFG----VPTRFGLTDEYA 386
Query 958 GSKPD----GTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLV------------- 1086
G P AM A GQ T QM AIAN+G ++EP +
Sbjct 387 GQLPADNIVNIAMSAFGQ-GISVTQTQMLRAFTAIANDGVMLEPKFISALYDPNDQSVRK 445
Query 1087 AQEGSAGNVISTHT-----EHQLSQAVSPATAQKLQQLMESVVQHGTG-TNAKIPGVTVG 1248
+Q+ GN +S +H + P ++ H TG +PG V
Sbjct 446 SQKEIVGNPVSKEAASVTRDHMVMVGTDPTYG--------TMYNHSTGKATVNVPGQNVA 497
Query 1249 GKTGTAQHGQDNSG 1290
K+GTA+ + +G
Sbjct 498 LKSGTAEIADEKNG 511
>4OON_A Chain A, Crystal Structure Of Pbp1a In Complex With Compound
17 ((4z,8s,11e, 14s)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-
Dihydro-2h-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-
Triazatetradeca-4,11-diene-2,12,14-tricarboxylic
Acid)
Length=795
Score = 46.6 bits (109), Expect = 7e-05, Method: Compositional matrix adjust.
Identities = 94/401 (23%), Positives = 150/401 (37%), Gaps = 87/401 (22%)
Frame = +1
Query 385 PKQGGDVVTTIDP-KVQKA-----GFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTF 546
P+Q DV D +VQ+ F + + A +++DPK GAI LV S++ +
Sbjct 363 PRQPADVAQAGDQIRVQRQEDGTLRFVQIPAAQSALISLDPKDGAIRSLVGGFSFEQSNY 422
Query 547 AGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDT 726
NRA++ PGS+FK + A + G + + S V D
Sbjct 423 -----------------------NRAIQAKRQPGSSFKPFIYSAALDNG-FTAASLVNDA 458
Query 727 P----DQYI----LPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLR 882
P D+Y+ P T G ++ A+ S N V ++ LG ++
Sbjct 459 PIVFVDEYLDKVWRPKNDTNTF-------LGPIPLREALYKSRNMVSIRVLQGLGIERAI 511
Query 883 AQAEKFGFNKE---------------VTIPIRADASHFPSGS-KPDGTAMDAI----GQH 1002
+ KFGF ++ + I S F +G K + ++ I GQ
Sbjct 512 SYITKFGFQRDELPRNFSLALGTATVTPMEIAGAWSVFANGGYKVNPYVIERIESRDGQV 571
Query 1003 DTRATP------LQMAMVAAAIANNGSLMEPYLVAQEGS------AGNVISTHTEHQLSQ 1146
+A P Q+A A N G P EGS A +T +
Sbjct 572 LYQANPPRVPVEEQVAADAEDAGNPGDPEHPESAEGEGSIEAQQVAAKAQTTFEPTPAER 631
Query 1147 AVSPATAQKLQQLMESVVQHGTGTNA-KIPGVTVGGKTGTAQHGQDNSGLPFAWFVSYAK 1323
+ TA + +++ V++ GTG A + + GKTGT +D WF Y
Sbjct 632 IIDARTAYIMTSMLQDVIKRGTGRRALALKRTDLAGKTGTTNDSKD------GWFSGY-- 683
Query 1324 GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
+V V + + ++ GG++A PI M AL
Sbjct 684 -NSDYVTSVWVGFDQPETLGRREYGGTVALPIWIRYMGFAL 723
>1PYY_A Chain A, Double Mutant Pbp2x T338aM339F FROM STREPTOCOCCUS PNEUMONIAE
STRAIN R6 At 2.4 A Resolution
Length=702
Score = 43.9 bits (102), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 86/366 (23%), Positives = 138/366 (38%), Gaps = 57/366 (16%)
Frame = +1
Query 295 SQLEKLEDGILSGTDSRLFFRNT--------LDMLTGKPKQGGDVVTTIDPKVQK----- 435
S +E + IL+GTD + + + ++ + G DV TTI +Q
Sbjct 169 SGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ 228
Query 436 --AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
A E + K A + KTG IL P++D T G T+D W + N
Sbjct 229 MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITED--FVWRDILYQSN-- 284
Query 610 MLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPL-INASPTE 786
Y PGS FK++ A A + + +V ++ + I T +N T
Sbjct 285 ---------YEPGSAFKVMMLAAAIDNNTFPG-GEVFNSSELKIADATIRDWDVNEGLTG 334
Query 787 DCGNATVQ---HAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPS 957
Q H+ ++ + KMG D L +F F +P R + +
Sbjct 335 GRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLN----RFKFG----VPTRFGLTDEYA 386
Query 958 GSKPD----GTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTH 1125
G P A + GQ T QM AIAN+G ++EP ++ + +
Sbjct 387 GQLPADNIVNIAQSSFGQ-GISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARK 445
Query 1126 TEHQL-SQAVSPATAQKLQQLME---------SVVQHGTGT-NAKIPGVTVGGKTGTAQH 1272
++ ++ VS A + M ++ H TG +PG V K+GTAQ
Sbjct 446 SQKEIVGNPVSKDAASLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQI 505
Query 1273 GQDNSG 1290
+ +G
Sbjct 506 ADEKNG 511
>1PMD_A Chain A, Penicillin-Binding Protein 2x (Pbp-2x)
Length=675
Score = 43.5 bits (101), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 86/366 (23%), Positives = 138/366 (38%), Gaps = 57/366 (16%)
Frame = +1
Query 295 SQLEKLEDGILSGTDSRLFFRNT--------LDMLTGKPKQGGDVVTTIDPKVQK----- 435
S +E + IL+GTD + + + ++ + G DV TTI +Q
Sbjct 142 SGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ 201
Query 436 --AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
A E + K A + KTG IL P++D T G T+D W + N
Sbjct 202 MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITED--FVWRDILYQSN-- 257
Query 610 MLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPL-INASPTE 786
Y PGST K++ A A + + +V ++ + I T +N T
Sbjct 258 ---------YEPGSTMKVMMLAAAIDNNTFPG-GEVFNSSELKIADATIRDWDVNEGLTG 307
Query 787 DCGNATVQ---HAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPS 957
Q H+ ++ + KMG D L +F F +P R + +
Sbjct 308 GRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLN----RFKFG----VPTRFGLTDEYA 359
Query 958 GSKPD----GTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTH 1125
G P A + GQ T QM AIAN+G ++EP ++ + +
Sbjct 360 GQLPADNIVNIAQSSFGQG-ISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARK 418
Query 1126 TEHQL-SQAVSPATAQKLQQLME---------SVVQHGTGT-NAKIPGVTVGGKTGTAQH 1272
++ ++ VS A + M ++ H TG +PG V K+GTAQ
Sbjct 419 SQKEIVGNPVSKDAASLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQI 478
Query 1273 GQDNSG 1290
+ +G
Sbjct 479 ADEKNG 484
>1QME_A Chain A, Penicillin-binding Protein 2x (pbp-2x)
1QMF_A Chain A, Penicillin-binding Protein 2x (pbp-2x) Acyl-enzyme Complex
Length=702
Score = 43.1 bits (100), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 86/366 (23%), Positives = 138/366 (38%), Gaps = 57/366 (16%)
Frame = +1
Query 295 SQLEKLEDGILSGTDSRLFFRNT--------LDMLTGKPKQGGDVVTTIDPKVQK----- 435
S +E + IL+GTD + + + ++ + G DV TTI +Q
Sbjct 169 SGMESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ 228
Query 436 --AGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKP 609
A E + K A + KTG IL P++D T G T+D W + N
Sbjct 229 MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITED--FVWRDILYQSN-- 284
Query 610 MLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPL-INASPTE 786
Y PGST K++ A A + + +V ++ + I T +N T
Sbjct 285 ---------YEPGSTMKVMMLAAAIDNNTFPG-GEVFNSSELKIADATIRDWDVNEGLTG 334
Query 787 DCGNATVQ---HAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNKEVTIPIRADASHFPS 957
Q H+ ++ + KMG D L +F F +P R + +
Sbjct 335 GRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLN----RFKFG----VPTRFGLTDEYA 386
Query 958 GSKPD----GTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSAGNVISTH 1125
G P A + GQ T QM AIAN+G ++EP ++ + +
Sbjct 387 GQLPADNIVNIAQSSFGQ-GISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARK 445
Query 1126 TEHQL-SQAVSPATAQKLQQLME---------SVVQHGTGT-NAKIPGVTVGGKTGTAQH 1272
++ ++ VS A + M ++ H TG +PG V K+GTAQ
Sbjct 446 SQKEIVGNPVSKDAASLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQI 505
Query 1273 GQDNSG 1290
+ +G
Sbjct 506 ADEKNG 511
>4YE5_A Chain A, The Crystal Structure Of A Peptidoglycan Synthetase
From Bifidobacterium Adolescentis Atcc 15703
4YE5_B Chain B, The Crystal Structure Of A Peptidoglycan Synthetase
From Bifidobacterium Adolescentis Atcc 15703
Length=566
Score = 42.7 bits (99), Expect = 8e-04, Method: Compositional matrix adjust.
Identities = 85/405 (21%), Positives = 158/405 (39%), Gaps = 55/405 (14%)
Frame = +1
Query 301 LEKLEDGILSGTDSRLFFR--NTLDMLTGKPKQ------GGDVVTTIDPKVQKAGFEGLG 456
+E+ E+ L+G D ++ N+ + G + G DV TID VQ + L
Sbjct 173 IEQXENKTLTGRDGYQVYQQGNSGVEIPGTXTESKDAVNGSDVTLTIDRDVQWYTEKVLS 232
Query 457 NKK-------GAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSDPNKPML 615
+ + G A D ++G IL L + + + G SD K
Sbjct 233 DSENKYHSAWGIAXVQDVQSGDILALADSDTTEAG-----------------SDQAKXGA 275
Query 616 NRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINASPTEDCG 795
+RA+ ET+ PGS K++ + + G ++ D++ +P T T + +D
Sbjct 276 SRAVSETFEPGSIGKVLAXSGXLQLGLHK-------IDDKFTVPNTVT--VEGQTYKDAV 326
Query 796 NATVQHAMDLSCNTVFGKMGAELGGDKLRAQAE-----KFGFNKE--VTIPIRADASHFP 954
+ +H +G + GDK + KFG + + +P ++ P
Sbjct 327 DHGNEHWTLAGILEQSSNVGXVIAGDKXTNEQRYNFISKFGIGQATGLNLPGESEGVLHP 386
Query 955 SGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQE--GSAGNVISTHT 1128
S S T + Q+ + IAN G + +P + + +AG+V +
Sbjct 387 SDSWDRRTRNTVLFGQGYTVNVXQLTNAISVIANKG-VKKPQRIIKSITDTAGHVEEQQS 445
Query 1129 EHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSGLPFAWF 1308
+ + ++ + + A + ES +H T K+ G K+GTA+ N L +
Sbjct 446 KGEATRVIDESVASQXLNAXESSAEH-YNTFVKVDGYRXAAKSGTAEVAGANGQLT-SII 503
Query 1309 VSYAK--GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
Y+ D + + VV++D ++ G + A I + + Q
Sbjct 504 SDYSTIIPADNPRFVITVVLKDPQGSFGGLTAGPVTAEIGEFLXQ 548
>3DWK_A Chain A, Identification Of Dynamic Structural Motifs Involved
In Peptidoglycan Glycosyltransfer
3DWK_B Chain B, Identification Of Dynamic Structural Motifs Involved
In Peptidoglycan Glycosyltransfer
3DWK_C Chain C, Identification Of Dynamic Structural Motifs Involved
In Peptidoglycan Glycosyltransfer
3DWK_D Chain D, Identification Of Dynamic Structural Motifs Involved
In Peptidoglycan Glycosyltransfer
Length=625
Score = 42.4 bits (98), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 69/303 (23%), Positives = 120/303 (40%), Gaps = 61/303 (20%)
Frame = +1
Query 400 DVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAW 579
DV T+ V F +++ A +D KTG ++ + G +D
Sbjct 271 DVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAI------------SGGRD----- 313
Query 580 TALDSDPNKPMLNR-ALRETYPPGSTFK-LVTAATAFETGKYQSPSDVTDTPDQYILPGT 753
K ++NR + +P GS+ K + A E K+ + + D Y + G+
Sbjct 314 -------FKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDES-SYQVDGS 365
Query 754 STPLINASPTEDCGNATVQHAMDLSCNT----VFGKMGAELGGDKLRAQAEKFGFNKEVT 921
+ T+ G ++ A+ S N + + G D + A K G N E
Sbjct 366 T---FRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGD 422
Query 922 IPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY----LVA 1089
I PS + +G + +P Q+A AAIAN G+ + +V
Sbjct 423 IG--------PS---------EVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVT 465
Query 1090 QEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQ-HGTGTNAKIPGVTVGGKTGTA 1266
++G + HT H +A+S TA L ++++ + +G+ + GV +G KTGT
Sbjct 466 RDGET--IEYDHTSH---KAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTG 520
Query 1267 QHG 1275
+G
Sbjct 521 TYG 523
>2Z2L_B Chain B, Penicillin-binding Protein 2x (pbp2x) From Streptococcus
Pneumoniae
2Z2L_E Chain E, Penicillin-binding Protein 2x (pbp2x) From Streptococcus
Pneumoniae
2Z2M_B Chain B, Cefditoren-acylated Penicillin-binding Protein 2x (pbp2x)
From Streptococcus Pneumoniae
2Z2M_E Chain E, Cefditoren-acylated Penicillin-binding Protein 2x (pbp2x)
From Streptococcus Pneumoniae
2ZC3_B Chain B, Penicillin-Binding Protein 2x (Pbp 2x) Acyl-Enzyme Complex
(Biapenem) From Streptococcus Pneumoniae
2ZC3_E Chain E, Penicillin-Binding Protein 2x (Pbp 2x) Acyl-Enzyme Complex
(Biapenem) From Streptococcus Pneumoniae
2ZC4_B Chain B, Penicillin-Binding Protein 2x (Pbp 2x) Acyl-Enzyme Complex
(Tebipenem) From Streptococcus Pneumoniae
2ZC4_E Chain E, Penicillin-Binding Protein 2x (Pbp 2x) Acyl-Enzyme Complex
(Tebipenem) From Streptococcus Pneumoniae
Length=385
Score = 40.4 bits (93), Expect = 0.004, Method: Compositional matrix adjust.
Identities = 79/334 (24%), Positives = 126/334 (38%), Gaps = 49/334 (15%)
Frame = +1
Query 367 DMLTGKPKQGGDVVTTIDPKVQK-------AGFEGLGNKKGAAVAIDPKTGAILGLVSTP 525
+ ++ + G DV TTI +Q A E + K A + KTG IL P
Sbjct 9 EQVSQRTMDGKDVYTTISSPLQSFMETQMDAFQEKVKGKYMTATLVSAKTGEILATTQRP 68
Query 526 SYDPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQS 705
++D T G T+D W + N Y PGST K++ A A + +
Sbjct 69 TFDADTKEGITED--FVWRDILYQSN-----------YEPGSTMKVMMLAAAIDNNTFPG 115
Query 706 PSDVTDTPDQYILPGTSTPL-INASPTEDCGNATVQ---HAMDLSCNTVFGKMGAELGGD 873
+V ++ + I T +N T Q H+ ++ + KMG D
Sbjct 116 -GEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGFAHSSNVGMTLLEQKMGDATWLD 174
Query 874 KLRAQAEKFGFNKEVTIPIRADASHFPSGSKPD----GTAMDAIGQHDTRATPLQMAMVA 1041
L +F F +P R + +G P A + GQ T QM
Sbjct 175 YLN----RFKFG----VPTRFGLTDEYAGQLPADNIVNIAQSSFGQG-ISVTQTQMIRAF 225
Query 1042 AAIANNGSLMEPYLVAQEGSAGNVISTHTEHQL-SQAVSPATAQKLQQLME--------- 1191
AIAN+G ++EP ++ + + ++ ++ VS A + M
Sbjct 226 TAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAASLTRTNMVLVGTDPVYG 285
Query 1192 SVVQHGTGT-NAKIPGVTVGGKTGTAQHGQDNSG 1290
++ H TG +PG V K+GTAQ + +G
Sbjct 286 TMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNG 319
Database: PDB protein database
Posted date: Mar 2, 2018 2:31 PM
Number of letters in database: 24,642,510
Number of sequences in database: 97,497
Lambda K H
0.314 0.131 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 97497
Number of Hits to DB: 8556081
Number of extensions: 233942
Number of successful extensions: 991
Number of sequences better than 10: 26
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 950
Number of HSP's successfully gapped: 42
Length of query: 1455
Length of database: 24642510
Length adjustment: 106
Effective length of query: 1349
Effective length of database: 14307828
Effective search space: 5422666812
Effective search space used: 5422666812
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 64 (29.3 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
QUESTIONS
- How is this output different to that from command-line and web-interface `BLAST` results?
- How many hits are there?
- What is the "best hit" to the query? Do you think it is a 'good' hit?
- At what point do you think the matches start to become less reliable? Why do you think this? (*HINT:* inspect the alignments)
Save BLASTX results to file¶
The results you obtianed above are human-readable, and similar to the default output type you saw from command-line/terminal BLAST in notebook 02. But, for now, they exist only in the variable called output. If we want to come back to these results at some time in the future, we will need to save them to a file somewhere.
This is a common operation in programmatic approaches to bioinformatics: once a result is obtained, we usually want to save it to a file. Most command-line programs will do this for you, but when working programmatically mostly you will need to save it explicitly, yourself.
The Python code for saving the contents of output to the file output/kitasatospora/remote_blastx_query_01.txt is given in the cell below:
In [6]:
#Â Make directory to save output, if it doesn't exist
outdir = os.path.join("output", "kitasatospora")
os.makedirs(outdir, exist_ok=True)
# Save output to file
outfilename = os.path.join(outdir, "remote_blastx_query_01.txt")
with open(outfilename, 'w') as outfh:
outfh.write(output)
This code does three main things:
- It creates a variable called
outfilename, with the path to the file we want to write - It opens that file, ready for writing, as a handle called
outfh - It writes the contents of
outputinto theoutfhhandle (and then closes the handle)
When this is done, the BLAST search results we got from NCBI are written to the file output/kitasatospora/remote_blastx_query_01.txt, just as though we did the search locally. You can inspect the contents of that file at the terminal using the command:
less output/kitasatospora/remote_blastx_query_01.txt
Using Entrez queries to modify the remote database¶
The remote NCBI BLAST+ service provides large, comprehensive databases such as nr, nt and refseq, but it doesn't provide very many specialised databases. Searches against large databases - where you don't care about most of the sequences, and most of the sequences are unlikely to match - can be wasteful and take longer than necessary to get the useful answer you're looking for. But a smaller, specialised organism-centric database - which would be much quicker to search - may not exist separately.
NCBI provide a service called `Entrez E-Utilities` which allows for complex text-based searches to be defined. A detailed consideration of this very powerful tool is beyond the scope of this course, but we will use the service to replicate the `Organism` field of the NCBI web browser interface that allowed us to restrict the `nr` database only to Kitasatospora sequences.
In notebook 01 you used the Organism field in the web browser interface to specify Kitasatospora (taxid: 2063) when querying against the complete nr database. This had the effect of only querying against sequences in nr that originated from Kitasatospora, reducing the overall search time and giving you the relevant hits you needed without producing a very large set of output.
To perform a similar filtering of the search using NCBIWWW.qblast(), you specify the argument entrez_query. The value to be passed is a string that describes the search field you want to filter on. There are many search field options (see the list here), but we will use only one in this lesson: the organism field.
The `Entrez` query syntax is the same syntax that you would use in any browser-based NCBI database search, and is reported back to you when you refine searches at NCBI. Looking at that output is a good way to find interesting ways to slice large databases into smaller subsets of interest.
The organism field [ORGN]¶
The syntax for filtering on organism is <value>[ORGN] where <value> is the term you want to filter on. This can be the name of the organism, or an identifier such as the NCBI taxon ID.
In the cell below, the remote search is conducted on the `nr` database, but restricted only to the set of sequences originating from *Kitasatospora*, by specififying `entrez_query="kitasatospora[ORGN]"`:
In [7]:
# Repeat the BLAST search on the nr database, restricted to Kitasatospora spp.
result_handle = NCBIWWW.qblast("blastx", "nr", seq.format("fasta"),
entrez_query="kitasatospora[ORGN]",
format_type="Text")
In [8]:
# Show human-readable results
output = result_handle.read()
print(output)
# Save output to file
outfilename = os.path.join(outdir, "remote_blastx_query_02.txt")
with open(outfilename, 'w') as outfh:
outfh.write(output)
<p><!--
QBlastInfoBegin
Status=READY
QBlastInfoEnd
--><p>
<PRE>
BLASTX 2.8.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: 9RXNN4JV015
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
147,386,088 sequences; 54,027,546,203 total letters
Query= lcl|LISX01000001.1_cds_OKJ16671.1_31 [locus_tag=AMK19_00175]
[protein=penicillin-binding protein] [protein_id=OKJ16671.1]
[location=39730..41184]
Length=1455
Score E
Sequences producing significant alignments: (Bits) Value
WP_073807071.1 penicillin-binding protein 2 [Kitasatospora sp... 996 0.0
WP_035864460.1 penicillin-binding protein [Kitasatospora chee... 946 0.0
WP_043915377.1 penicillin-binding protein 2 [Kitasatospora gr... 897 0.0
WP_100589593.1 penicillin-binding protein [Kitasatospora sp. ... 896 0.0
WP_014137042.1 penicillin-binding protein 2 [Kitasatospora se... 855 0.0
WP_033254180.1 penicillin-binding protein 2 [Kitasatospora ph... 853 0.0
WP_033214154.1 penicillin-binding protein 2 [Kitasatospora ph... 847 0.0
WP_030457661.1 penicillin-binding protein 2 [Kitasatospora sp... 832 0.0
WP_033820252.1 penicillin-binding protein 2 [Kitasatospora sp... 820 0.0
WP_100889221.1 penicillin-binding protein 2 [Kitasatospora sp... 770 0.0
WP_049656873.1 penicillin-binding protein 2 [Kitasatospora sp... 766 0.0
AUG78584.1 penicillin-binding protein [Kitasatospora sp. MMS1... 764 0.0
WP_057228636.1 MULTISPECIES: penicillin-binding protein 2 [Ki... 764 0.0
WP_030392400.1 MULTISPECIES: penicillin-binding protein 2 [Ki... 736 0.0
WP_030278231.1 MULTISPECIES: penicillin-binding protein 2 [St... 725 0.0
WP_033346841.1 penicillin-binding protein 2 [Kitasatospora au... 724 0.0
WP_035799332.1 penicillin-binding protein [Kitasatospora medi... 712 0.0
WP_035847202.1 penicillin-binding protein [Kitasatospora azat... 704 0.0
WP_084747517.1 penicillin-binding protein [Kitasatospora albo... 548 0.0
WP_003978842.1 MULTISPECIES: penicillin-binding protein [Stre... 541 0.0
WP_052836711.1 penicillin-binding protein 2 [Kitasatospora au... 516 1e-180
WP_003985178.1 MULTISPECIES: penicillin-binding protein [Stre... 514 1e-179
WP_052841852.1 penicillin-binding protein 2 [Kitasatospora au... 491 7e-171
WP_084745269.1 penicillin-binding protein [Kitasatospora albo... 481 7e-167
WP_051969980.1 penicillin-binding protein [Kitasatospora azat... 461 1e-158
WP_030393477.1 MULTISPECIES: penicillin-binding protein [Kita... 447 1e-153
WP_051966736.1 penicillin-binding protein [Kitasatospora medi... 446 1e-152
AUG79326.1 Putative penicillin-binding protein A [Kitasatospo... 445 4e-152
AUG79205.1 penicillin-binding protein [Kitasatospora sp. MMS1... 441 3e-151
WP_084746985.1 penicillin-binding protein 2 [Kitasatospora al... 440 1e-150
WP_003981922.1 MULTISPECIES: penicillin-binding protein 2 [St... 439 2e-150
WP_052836864.1 penicillin-binding protein 2 [Kitasatospora au... 436 4e-149
WP_078656350.1 penicillin-binding protein [Kitasatospora aure... 435 1e-148
OEV33654.1 penicillin-binding protein [Kitasatospora aureofac... 432 1e-147
WP_078938872.1 MULTISPECIES: penicillin-binding protein [Stre... 432 1e-147
BAJ30397.1 putative penicillin-binding protein A [Kitasatospo... 431 6e-147
WP_057228032.1 MULTISPECIES: penicillin-binding protein [Kita... 420 4e-143
WP_051055920.1 penicillin-binding protein [Kitasatospora setae] 420 5e-143
WP_033819927.1 penicillin-binding protein [Kitasatospora sp. ... 419 1e-142
WP_051817654.1 penicillin-binding protein [Kitasatospora sp. ... 413 2e-140
WP_051776935.1 penicillin-binding protein [Kitasatospora phos... 412 5e-140
WP_052840719.1 penicillin-binding protein [Kitasatospora aure... 412 1e-139
WP_051732513.1 penicillin-binding protein [Kitasatospora phos... 410 5e-139
WP_100892658.1 penicillin-binding protein [Kitasatospora sp. ... 408 4e-138
PKB48172.1 peptidoglycan glycosyltransferase [Kitasatospora s... 408 9e-138
WP_082346127.1 penicillin-binding protein [Kitasatospora sp. ... 406 5e-137
WP_003983996.1 MULTISPECIES: penicillin-binding protein [Stre... 395 4e-133
WP_083646743.1 penicillin-binding protein [Kitasatospora sp. ... 395 6e-133
KIQ64169.1 penicillin-binding protein [Kitasatospora griseola] 389 1e-130
KDN83954.1 penicillin-binding protein [Kitasatospora cheerisa... 387 8e-130
ALIGNMENTS
>WP_073807071.1 penicillin-binding protein 2 [Kitasatospora sp. CB01950]
OKJ16671.1 penicillin-binding protein [Kitasatospora sp. CB01950]
Length=484
Score = 996 bits (2574), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 483/484 (99%), Positives = 484/484 (100%), Gaps = 0/484 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN
Sbjct 61 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT
Sbjct 181 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF
Sbjct 241 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 300
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY
Sbjct 301 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 360
Query 1081 LVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTG 1260
LVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTG
Sbjct 361 LVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTG 420
Query 1261 TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA 1440
TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA
Sbjct 421 TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA 480
Query 1441 ALGK 1452
ALGK
Sbjct 481 ALGK 484
>WP_035864460.1 penicillin-binding protein [Kitasatospora cheerisanensis]
KDN84196.1 penicillin-binding protein [Kitasatospora cheerisanensis KCTC
2395]
Length=484
Score = 946 bits (2444), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 454/484 (94%), Positives = 471/484 (97%), Gaps = 0/484 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALMLRVNWVQGVQAS WANNPHNDR KYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMLRVNWVQGVQASDWANNPHNDRAKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN
Sbjct 61 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLG+KKGAAVAIDP+TGAILGLVSTPSYDP
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGDKKGAAVAIDPRTGAILGLVSTPSYDPS 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGGT +D KAWTAL++DPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQ P+D T
Sbjct 181 TFAGGTDEDGKAWTALNNDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQKPTDAT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTPDQYILPGTSTPLINASPTEDCG+ATVQHAMDLSCNTVFGKMGAELGGD LRAQAEKF
Sbjct 241 DTPDQYILPGTSTPLINASPTEDCGDATVQHAMDLSCNTVFGKMGAELGGDALRAQAEKF 300
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFNKEV IP+RADASHFPS SKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY
Sbjct 301 GFNKEVNIPVRADASHFPSNSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 360
Query 1081 LVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTG 1260
LVAQEGSAGNVISTHTE QLSQAV+PATAQKLQQLMESVVQ+GTG NA+IPGVTVGGKTG
Sbjct 361 LVAQEGSAGNVISTHTERQLSQAVTPATAQKLQQLMESVVQNGTGKNARIPGVTVGGKTG 420
Query 1261 TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA 1440
TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDG+ E+++ISGGSLAAPIAKAMMQA
Sbjct 421 TAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGAGESREISGGSLAAPIAKAMMQA 480
Query 1441 ALGK 1452
ALGK
Sbjct 481 ALGK 484
>WP_043915377.1 penicillin-binding protein 2 [Kitasatospora griseola]
KIQ63037.1 penicillin-binding protein [Kitasatospora griseola]
Length=485
Score = 897 bits (2318), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 436/485 (90%), Positives = 454/485 (94%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALMLRVNWVQGVQAS WANNPHNDRTKYD+YAYPRGNII GGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMLRVNWVQGVQASAWANNPHNDRTKYDRYAYPRGNIIAGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVT+SDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSG DSRLFFRN
Sbjct 61 AVTRSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGADSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLG+KKGAAVA+DP+TGAILGLVSTPSYDPG
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGDKKGAAVALDPRTGAILGLVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TF+GGTKDDE AW AL+ DPNKP+LNRALRETYPPGSTFKLVTAATAFE GKYQ P+D T
Sbjct 181 TFSGGTKDDENAWKALNDDPNKPLLNRALRETYPPGSTFKLVTAATAFENGKYQKPTDTT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTP+ Y+LPGTST L NAS TE C NATVQ AMD SCNTVFGKMGAELGGDKLRAQAEKF
Sbjct 241 DTPNPYVLPGTSTALRNASATEPCENATVQVAMDYSCNTVFGKMGAELGGDKLRAQAEKF 300
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN ++ IPI S+FP SKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP
Sbjct 301 GFNSDQLRIPIAPTPSYFPKDSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQ+GTGT AKIPG TVGGKT
Sbjct 361 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQNGTGTKAKIPGATVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDN+GLPFAWFVSYAKGQDGKQVAVAVVVEDG SE +ISGGSLAAPIAKAMMQ
Sbjct 421 GTAQHGQDNAGLPFAWFVSYAKGQDGKQVAVAVVVEDGDSERNEISGGSLAAPIAKAMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_100589593.1 penicillin-binding protein [Kitasatospora sp. CB02891]
PJN21785.1 penicillin-binding protein [Kitasatospora sp. CB02891]
Length=485
Score = 896 bits (2315), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 436/485 (90%), Positives = 453/485 (93%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALMLRVNWVQGVQAS WANNPHNDRTKYD+YAYPRGNII GGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMLRVNWVQGVQASAWANNPHNDRTKYDRYAYPRGNIIAGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVT+SDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSG DSRLFFRN
Sbjct 61 AVTRSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGADSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLG+KKGAAVAIDP+TGAILGLVSTPSYDPG
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGDKKGAAVAIDPRTGAILGLVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TF+GGTKDDE AW AL+ DPNKP+LNRALRETYPPGSTFKLVTAA AFE GKYQ P+D T
Sbjct 181 TFSGGTKDDENAWKALNDDPNKPLLNRALRETYPPGSTFKLVTAAAAFENGKYQKPTDTT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTP+ Y+LPGTST L NAS TE C NATVQ AMD SCNTVFGKMGAELGGDKLRAQAEKF
Sbjct 241 DTPNPYVLPGTSTALRNASATEPCENATVQVAMDYSCNTVFGKMGAELGGDKLRAQAEKF 300
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN ++ IPI S+FP SKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP
Sbjct 301 GFNSDQLRIPIAPTPSYFPKDSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQ+GTGT AKIPG TVGGKT
Sbjct 361 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQNGTGTKAKIPGATVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDN+GLPFAWFVSYAKGQDGKQVAVAVVVEDG SE +ISGGSLAAPIAKAMMQ
Sbjct 421 GTAQHGQDNAGLPFAWFVSYAKGQDGKQVAVAVVVEDGDSERNEISGGSLAAPIAKAMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_014137042.1 penicillin-binding protein 2 [Kitasatospora setae]
BAJ29737.1 putative penicillin-binding protein A [Kitasatospora setae KM-6054]
Length=485
Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 409/485 (84%), Positives = 448/485 (92%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+RVNWVQGVQA+ WANN HN+R KYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRVNWVQGVQAAAWANNTHNERVKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYKRSWV+GP+YAPVTGYSSQ +DASQLEKLEDG+LSGTDSRLFFRN
Sbjct 61 PVTKSDFVNGLRYKYKRSWVEGPLYAPVTGYSSQIFDASQLEKLEDGVLSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN+KGAAVAIDP+TGAIL +VSTPSYDPG
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNRKGAAVAIDPRTGAILAVVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TF+GGT D A+ AL DPN+P++NRALRETYPPGSTFKL+TAATAFETGKYQ+P+D T
Sbjct 181 TFSGGTDADSAAYKALQDDPNRPLVNRALRETYPPGSTFKLITAATAFETGKYQNPNDTT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TP+ Y+LPGT+TPL NAS TE C +ATV AMD SCNTVFGKMGAELG D+LRAQAEKF
Sbjct 241 QTPNPYVLPGTNTPLRNASATEPCEDATVMVAMDYSCNTVFGKMGAELGRDQLRAQAEKF 300
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + V IPIRA AS++PSGS PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM+P
Sbjct 301 GFNNDKVDIPIRATASYYPSGSSPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMQP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLVAQEG+AGN ISTH+EHQ+SQAVSPATAQKLQQLMESVVQ+GTG A IPG+TVGGKT
Sbjct 361 YLVAQEGAAGNAISTHSEHQMSQAVSPATAQKLQQLMESVVQNGTGKKAAIPGLTVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDNSGLPFAWFVSYAK GKQVAVAVVVEDG++E+ +ISGGSLAAPIAK+MMQ
Sbjct 421 GTAQHGQDNSGLPFAWFVSYAKDASGKQVAVAVVVEDGAAESAEISGGSLAAPIAKSMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_033254180.1 penicillin-binding protein 2 [Kitasatospora phosalacinea]
Length=485
Score = 853 bits (2203), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 409/485 (84%), Positives = 445/485 (92%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILAL++RVNWVQGVQAS WANN N+RTKYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLILALLVRVNWVQGVQASAWANNKENERTKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYKR+WVDGPMYAPVTGYSSQ YDASQLEKLEDG+LSGTDSRLFFRN
Sbjct 61 PVTKSDFVNGLRYKYKRAWVDGPMYAPVTGYSSQIYDASQLEKLEDGVLSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN+KGAAVAIDP TGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNRKGAAVAIDPNTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGGT D A+ AL +DPN+PMLNRALR+TYPPGSTFKLVTAATAFETGKY+ P D T
Sbjct 181 TFAGGTDADSAAYNALKADPNQPMLNRALRQTYPPGSTFKLVTAATAFETGKYKDPGDAT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
+TP+ YILPGTSTPLIN SP EDC NAT+ A+ +SCNTVFGK+GAELGGD LRAQAEKF
Sbjct 241 ETPETYILPGTSTPLINDSPDEDCENATLTSALAISCNTVFGKLGAELGGDALRAQAEKF 300
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + V IP+RA AS++PSGS PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM+P
Sbjct 301 GFNDDKVDIPVRAAASYYPSGSSPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMQP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLV+QEG+ G+ ISTH+EHQ+SQAVSPATAQKLQQ+ME VV+ GTG AKIPGVTVGGKT
Sbjct 361 YLVSQEGAPGHAISTHSEHQMSQAVSPATAQKLQQMMEEVVKSGTGKRAKIPGVTVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDNSG PFAWFVSYAK GKQVAVAVVVEDG++++ +ISGGSLAAPIAKAMMQ
Sbjct 421 GTAQHGQDNSGKPFAWFVSYAKDSGGKQVAVAVVVEDGAAQSAEISGGSLAAPIAKAMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_033214154.1 penicillin-binding protein 2 [Kitasatospora phosalacinea]
Length=485
Score = 847 bits (2188), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 405/485 (84%), Positives = 446/485 (92%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+RVNWVQGVQAS WA+NPHN+RTKYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRVNWVQGVQASAWASNPHNERTKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQ +DASQLEKLED +LSGTDSRLFFRN
Sbjct 61 PVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQVFDASQLEKLEDDVLSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGK +QGGDVVTTIDPKVQKAGFEGLGN+KGAAVAIDP+TGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGKTRQGGDVVTTIDPKVQKAGFEGLGNRKGAAVAIDPRTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGG+ D AWTAL SDPN+PMLNRALR+TYPPGSTFKLVTAATAFETGK++ P+D T
Sbjct 181 TFAGGSDADSAAWTALKSDPNQPMLNRALRQTYPPGSTFKLVTAATAFETGKFKDPNDET 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TP+ YILPGTSTPL+N SP E C +AT+ AM LSCNTVFGK+GAELGGD +RAQAEKF
Sbjct 241 QTPETYILPGTSTPLVNDSPDEPCTDATLAAAMALSCNTVFGKIGAELGGDAVRAQAEKF 300
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + V +PIRA AS++PSGS PDGTAMDAIGQHDTRATPL+MAMVAAAIANNGSLM+P
Sbjct 301 GFNNDKVDVPIRAAASYYPSGSSPDGTAMDAIGQHDTRATPLEMAMVAAAIANNGSLMQP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLVAQEG+ G+ ISTH+EHQ+SQAVSPATAQKLQQLME VV++GTG A IPGVTVGGKT
Sbjct 361 YLVAQEGAPGHAISTHSEHQMSQAVSPATAQKLQQLMEGVVKNGTGQRANIPGVTVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDN+G PFAWFVSYAK GKQVAVAVVVEDG++++ +ISGGSLAAPIAK+MMQ
Sbjct 421 GTAQHGQDNAGKPFAWFVSYAKDSSGKQVAVAVVVEDGAAQSSEISGGSLAAPIAKSMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_030457661.1 penicillin-binding protein 2 [Kitasatospora sp. NRRL B-11411]
Length=485
Score = 832 bits (2150), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 407/485 (84%), Positives = 444/485 (92%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVL+LALM+RVNWVQGVQAS WA NPHNDR KYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSIFCLVLVLALMVRVNWVQGVQASAWAGNPHNDRVKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQ YDASQLEKLED +LSGTDSRLFFRN
Sbjct 61 PVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQIYDASQLEKLEDPVLSGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN+KGAAVAIDP+TGAIL LVSTPSYDP
Sbjct 121 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNRKGAAVAIDPRTGAILALVSTPSYDPS 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGG D AWTAL +DPN+PMLNRALR+TYPPGSTFKLVTAATAFETGK+++P+D T
Sbjct 181 TFAGGGDSDSAAWTALQNDPNQPMLNRALRQTYPPGSTFKLVTAATAFETGKFKNPTDTT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTP+ YILPGTSTPL+N SP E C NAT+ AM +SCNTVFGK+GAELG D LR+QAEKF
Sbjct 241 DTPETYILPGTSTPLVNDSPDEPCTNATLASAMAISCNTVFGKIGAELGKDALRSQAEKF 300
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + V +PIRA AS++PSGS PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM+P
Sbjct 301 GFNNDKVDVPIRAAASYYPSGSSPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMQP 360
Query 1078 YLVAQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
YLV+QEGSAGN ISTH+EHQLSQAVSP TAQKLQQLME+VV+ GTG A IPGVTVGGKT
Sbjct 361 YLVSQEGSAGNAISTHSEHQLSQAVSPDTAQKLQQLMEAVVKDGTGRRAAIPGVTVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQDN+G PFAWFVSYAK GKQVAVAVVVEDG++++ +ISGGS+AAPIAK+MMQ
Sbjct 421 GTAQHGQDNAGKPFAWFVSYAKDSGGKQVAVAVVVEDGAAQSSEISGGSIAAPIAKSMMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>WP_033820252.1 penicillin-binding protein 2 [Kitasatospora sp. MBT63]
Length=486
Score = 820 bits (2117), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 393/486 (81%), Positives = 435/486 (90%), Gaps = 2/486 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+RVNWVQGVQA WA+N HNDR KYD+YAYPRGNII GGQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRVNWVQGVQAEAWASNKHNDRNKYDRYAYPRGNIIAGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT SDFVNGLRYKYKRSW+DGPMYAP+TGYSSQ++ SQLE+LEDG+L+GTDSRLFFRN
Sbjct 61 PVTNSDFVNGLRYKYKRSWIDGPMYAPITGYSSQSFGTSQLEQLEDGVLAGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG+ K+GGDVVTTIDPK QKA FEGLG+KKGAAVAIDP+TGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGEQKKGGDVVTTIDPKAQKAAFEGLGDKKGAAVAIDPRTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TF+GG K+D KAWT L++DPNKPMLNRALRETYPPGSTFKL+TAATAFETG Y++ D T
Sbjct 181 TFSGGGKEDSKAWTDLNNDPNKPMLNRALRETYPPGSTFKLITAATAFETGLYKNVDDAT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TP +ILPGT+T L NAS TEDC NATV+H MDLSCNTVFGKMGA+LG D+LRAQAEKF
Sbjct 241 QTPKPFILPGTNTELKNASATEDCENATVEHGMDLSCNTVFGKMGADLGKDRLRAQAEKF 300
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN ++ PIRA S FPS S PDGTAMDAIGQHDTRATPLQMAMVAAAI NNGSLM+P
Sbjct 301 GFNNGKIDTPIRAVTSVFPSNSSPDGTAMDAIGQHDTRATPLQMAMVAAAICNNGSLMQP 360
Query 1078 YLVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
YL+AQE SA +VIS HTEHQLSQAVSPATAQKLQQLMESVVQ+GTGT AKI G TVGGK
Sbjct 361 YLIAQERSANLDVISNHTEHQLSQAVSPATAQKLQQLMESVVQNGTGTKAKINGATVGGK 420
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMM 1434
TGTAQHGQDNSGLPFAWF+SYAKG DGKQVAVAVVVEDG++E +ISGGS+AAPIAK++M
Sbjct 421 TGTAQHGQDNSGLPFAWFMSYAKGADGKQVAVAVVVEDGAAERNEISGGSIAAPIAKSVM 480
Query 1435 QAALGK 1452
+A + K
Sbjct 481 EAVVNK 486
>WP_100889221.1 penicillin-binding protein 2 [Kitasatospora sp. OK780]
PKB48640.1 peptidoglycan glycosyltransferase [Kitasatospora sp. OK780]
Length=486
Score = 770 bits (1989), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 382/485 (79%), Positives = 427/485 (88%), Gaps = 4/485 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVS+FCLVL+LAL++R NWVQGVQA WA+N HNDR KYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSVFCLVLVLALLVRTNWVQGVQAQAWASNQHNDRNKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT SDFVNGLRYKYKRSWVDGPMYAPVTGYSSQ++ SQLE LEDGIL+GTDSRLFFRN
Sbjct 61 PVTNSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQSFGTSQLESLEDGILAGTDSRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG K+GG+VVTTI+ K QKA FEGLGNKKGAAVAIDP+TGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGGDKKGGNVVTTINAKAQKAAFEGLGNKKGAAVAIDPRTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TF+G T D KAWT L++DPNKPMLNRALRETYPPGSTFKL+TAA AFETG +Q+P D T
Sbjct 181 TFSGSTAADAKAWTDLNADPNKPMLNRALRETYPPGSTFKLITAAAAFETGMFQNPEDKT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
+TP+ Y LPGT T L NAS TE C NAT+ MD SCNTVFGK+GA+LG DKLRAQAEKF
Sbjct 241 ETPNPYTLPGTKTVLKNASETEPCENATLLVGMDFSCNTVFGKIGADLGKDKLRAQAEKF 300
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN +V +PIR S FP+ S PDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM+P
Sbjct 301 GFNNSQVDVPIRPSTSVFPTNSSPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMQP 360
Query 1078 YLVAQEGSAGN--VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGG 1251
YLV QE AGN V+S H+E QLSQAVSPATAQKLQQLMESVV++GTGT A+I GVTVGG
Sbjct 361 YLVDQE-QAGNLAVVSKHSEKQLSQAVSPATAQKLQQLMESVVKNGTGTKAQIDGVTVGG 419
Query 1252 KTGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAM 1431
KTGTAQHGQDNSGLPFAWF+SYAKG DGKQVAVAVVVEDG++E+++ISGGS+AAPIAK++
Sbjct 420 KTGTAQHGQDNSGLPFAWFMSYAKGADGKQVAVAVVVEDGAAESREISGGSIAAPIAKSV 479
Query 1432 MQAAL 1446
M+A +
Sbjct 480 MEAVV 484
>WP_049656873.1 penicillin-binding protein 2 [Kitasatospora sp. MY 5-36]
Length=485
Score = 766 bits (1977), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 369/485 (76%), Positives = 412/485 (85%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+R NWVQGVQA +WA N +N R YD+YA+PRGNIIV GQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRTNWVQGVQADSWATNKNNKRQLYDRYAHPRGNIIVSGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQ Y +SQLE LEDGILSGTD RLFFRN
Sbjct 61 PVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQAYGSSQLENLEDGILSGTDDRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG+ K+GGDVVTTI+ KVQKA F+ +GNKKGA VA+DPKTGAILGLVSTPSYDPG
Sbjct 121 TLDMLTGEQKRGGDVVTTINAKVQKAAFDAMGNKKGAVVALDPKTGAILGLVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGG D KAWT L++DPNKPMLNRAL+ETYPPGSTFKLVTA AFE G+YQS D T
Sbjct 181 TFAGGEAADTKAWTDLNADPNKPMLNRALKETYPPGSTFKLVTATAAFENGRYQSIDDAT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTPD ++LPGT+T L N SP E C NATV+ MD SCNTVF KMGA+LG +++RAQAEKF
Sbjct 241 DTPDPWVLPGTTTQLKNESPNERCENATVKSGMDQSCNTVFAKMGADLGKERMRAQAEKF 300
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFN + P+R++ S FP S PDGTA D+IGQHDTRATPLQMAMV+AAIANNGSLM+PY
Sbjct 301 GFNSTIDTPVRSEKSVFPDSSSPDGTAQDSIGQHDTRATPLQMAMVSAAIANNGSLMQPY 360
Query 1081 LVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
LV QE SA +S HTE Q SQA+SPATAQK+Q LM SVV++GTGTNAKIPG+ VGGKT
Sbjct 361 LVDQERSATLTTVSKHTEKQFSQAMSPATAQKMQDLMVSVVENGTGTNAKIPGLQVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHG+DN+GLPFAWF S+AK DG+ VAVAVVVEDGS ISGG LAAPIAKA+MQ
Sbjct 421 GTAQHGEDNAGLPFAWFTSWAKTPDGQSVAVAVVVEDGSQNRDGISGGRLAAPIAKAVMQ 480
Query 1438 AALGK 1452
AALGK
Sbjct 481 AALGK 485
>AUG78584.1 penicillin-binding protein [Kitasatospora sp. MMS16-BH015]
Length=485
Score = 764 bits (1974), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 377/484 (78%), Positives = 412/484 (85%), Gaps = 2/484 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVS+FCLVLILALM+R NWVQGVQA TWA+N HNDR KYD+YAYPRGNIIV G
Sbjct 1 MNKPIRRVSVFCLVLILALMVRTNWVQGVQADTWASNSHNDRNKYDRYAYPRGNIIVQGN 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VTKSDFVNGLRYKYK+SWVDGPMYAPVTG+SSQ +D+SQLEKLED ILSGTDSRLF RN
Sbjct 61 PVTKSDFVNGLRYKYKQSWVDGPMYAPVTGWSSQAFDSSQLEKLEDPILSGTDSRLFLRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGK KQGGDVVTTI+ K QKA FEGLG+KKGAAVAIDP TGAIL LVSTPSYDP
Sbjct 121 TLDMLTGKQKQGGDVVTTINAKAQKAAFEGLGDKKGAAVAIDPATGAILALVSTPSYDPS 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
TFAGG D A ALD DPN+P+LNRALRETYPPGSTFKL+TAA AFE G++QS D T
Sbjct 181 TFAGGATADGNAKAALDKDPNQPLLNRALRETYPPGSTFKLITAAAAFENGRFQSIDDTT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTP YILPGT+T L NAS TE C NATV+ MD SCNTVFGK+GA+LG DKLR QAEKF
Sbjct 241 DTPAHYILPGTNTELKNASDTEPCPNATVKTGMDFSCNTVFGKIGADLGKDKLRGQAEKF 300
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + PIRA S+FP S PDGTAMDAIGQHDTRATPLQMAMVA+AI NNGSLM P
Sbjct 301 GFNNPALDTPIRAVPSYFPPNSSPDGTAMDAIGQHDTRATPLQMAMVASAICNNGSLMTP 360
Query 1078 YLVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
YLV QE SA VIS HT QLSQAVSP TAQKLQQLMESVV +GTGT A+I G+TVGGK
Sbjct 361 YLVEQERSANLTVISKHTPKQLSQAVSPGTAQKLQQLMESVVANGTGTKAQIDGLTVGGK 420
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMM 1434
TGTAQHGQDN+GLPFAWF+SYAKG D K VAVAVVVEDG++E +ISGGS+AAPIAK++M
Sbjct 421 TGTAQHGQDNAGLPFAWFMSYAKGPDNKSVAVAVVVEDGAAERNEISGGSIAAPIAKSVM 480
Query 1435 QAAL 1446
+A L
Sbjct 481 EAVL 484
>WP_057228636.1 MULTISPECIES: penicillin-binding protein 2 [Kitasatospora]
KQV22225.1 penicillin-binding protein [Kitasatospora sp. Root107]
KRB64622.1 penicillin-binding protein [Kitasatospora sp. Root187]
Length=487
Score = 764 bits (1973), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 366/487 (75%), Positives = 419/487 (86%), Gaps = 3/487 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIV-GG 177
+NKPIRRVSIFCLVL+LALM+RVNWVQGVQA WA+NPHNDR KYD+YAYPRGNIIV GG
Sbjct 1 MNKPIRRVSIFCLVLVLALMVRVNWVQGVQAEAWASNPHNDRNKYDRYAYPRGNIIVAGG 60
Query 178 QAVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFR 357
Q +T+SDFVNG RYKYKR+WVDGP++APVTGYSSQ Y SQLE L DGILSG+DSRLFFR
Sbjct 61 QPITRSDFVNGYRYKYKRAWVDGPLWAPVTGYSSQAYTTSQLESLSDGILSGSDSRLFFR 120
Query 358 NTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDP 537
NTLDMLTGK KQGGDVVTT++ K QKA ++ LG+KKGAAVAIDP+TGAIL LVSTPSYDP
Sbjct 121 NTLDMLTGKEKQGGDVVTTLNAKAQKAAYDALGSKKGAAVAIDPRTGAILALVSTPSYDP 180
Query 538 GTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDV 717
G FAG +DEKAW L++DPNKPMLNRALRETYPPGSTFKLVTAA AFE+G+YQ+ DV
Sbjct 181 GRFAGTGAEDEKAWNELNADPNKPMLNRALRETYPPGSTFKLVTAAAAFESGRYQNIEDV 240
Query 718 TDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEK 897
TDTP +Y+LP T+ L NAS E C NAT++ AMD SCNTVFGK+GA+LGG+K+R QAE+
Sbjct 241 TDTPQRYVLPNTNVELKNASDDEPCANATLKVAMDYSCNTVFGKIGADLGGEKMRTQAER 300
Query 898 FGFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLME 1074
FGFN + +PIRA S FPS S DGTAMD+IGQHDTRATPLQMAMVAAAIAN+G LM
Sbjct 301 FGFNNPGINVPIRASESFFPSKSSADGTAMDSIGQHDTRATPLQMAMVAAAIANDGRLMA 360
Query 1075 PYLVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGG 1251
PY+V +E ++ IS H+E QLSQAVSPATAQKLQQLMESVVQ+GTG A+I G+ VGG
Sbjct 361 PYMVDEERASNLTTISKHSERQLSQAVSPATAQKLQQLMESVVQNGTGKKAQIDGLKVGG 420
Query 1252 KTGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAM 1431
KTGTAQHG++N+GLPFAWF+SYAK DG+QVAVAVVVEDGSSE QISGG LAAPIAKA+
Sbjct 421 KTGTAQHGENNAGLPFAWFMSYAKAPDGRQVAVAVVVEDGSSERDQISGGGLAAPIAKAL 480
Query 1432 MQAALGK 1452
M+AAL +
Sbjct 481 MKAALDR 487
>WP_030392400.1 MULTISPECIES: penicillin-binding protein 2 [Kitasatospora]
Length=485
Score = 736 bits (1901), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 357/485 (74%), Positives = 401/485 (83%), Gaps = 1/485 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+R NWVQGVQA WA N +N R YD+YAYPRGNIIV GQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRTNWVQGVQADAWAGNKYNKRQVYDRYAYPRGNIIVDGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
+T+S FV+GLRYKYKRSW D MYAPVTGYSSQ + ASQLE+LEDG+LSG+D RLFFRN
Sbjct 61 PITQSTFVDGLRYKYKRSWTDPQMYAPVTGYSSQAFGASQLEQLEDGVLSGSDDRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG+ K+GGDVVTTI+ K QKA FEGLGNKKGA VA+DPKTGAIL LVSTPSYDP
Sbjct 121 TLDMLTGEQKRGGDVVTTINSKAQKAAFEGLGNKKGAVVALDPKTGAILALVSTPSYDPA 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
+FAG D KAWT L++DPNKPMLNRALRETYPPGSTFKLVTA+ AFE G YQ+ D T
Sbjct 181 SFAGNESTDSKAWTDLNADPNKPMLNRALRETYPPGSTFKLVTASAAFENGLYQNIDDQT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TPD Y LPGT+ L N S E CGNAT++ MD SCNTVF K+GA+LG DK+R QAEKF
Sbjct 241 ATPDPYTLPGTNFVLKNESDREQCGNATIKSGMDQSCNTVFAKIGADLGKDKMRVQAEKF 300
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFN V PIR++ S FPS S PDGTA D+IGQHDT+ATPLQMAMV+AAIANNGSLM+PY
Sbjct 301 GFNSTVDTPIRSEKSVFPSNSTPDGTAQDSIGQHDTQATPLQMAMVSAAIANNGSLMQPY 360
Query 1081 LVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
LVA+E SA IS H+E Q SQ +SPATAQK+Q LM SVV++GTG NAKIPGVTVGGKT
Sbjct 361 LVAEERSASLTTISKHSEKQFSQPISPATAQKMQDLMVSVVENGTGKNAKIPGVTVGGKT 420
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHG+ N+GLPFAWF SYAK DG+ VAVAVVVEDGS+ ISGG LAAPIAK++MQ
Sbjct 421 GTAQHGEGNAGLPFAWFTSYAKASDGRTVAVAVVVEDGSNNRDGISGGRLAAPIAKSVMQ 480
Query 1438 AALGK 1452
A LGK
Sbjct 481 AVLGK 485
>WP_030278231.1 MULTISPECIES: penicillin-binding protein 2 [Streptomycetaceae]
OEV37885.1 penicillin-binding protein [Kitasatospora aureofaciens]
ARF79435.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=483
Score = 725 bits (1872), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 355/485 (73%), Positives = 400/485 (82%), Gaps = 3/485 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+R NWVQGVQA WA+N +N R YD+YA+PRGNIIV GQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRTNWVQGVQADAWASNKNNKRQLYDRYAHPRGNIIVSGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT SDFVNGLRYKYKRSW++GPMYAPVTGYSSQ Y ++ LE LEDGILSGTD RLFFRN
Sbjct 61 PVTNSDFVNGLRYKYKRSWINGPMYAPVTGYSSQAYGSNGLENLEDGILSGTDDRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG+ ++GGDVVTTI+ KVQKA F+ +G+KKGA VA+DPKTGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGEQQRGGDVVTTINAKVQKAAFDAMGDKKGAVVALDPKTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
T AG DD AW AL+SDPNKPM NRAL+ TYPPGSTFKLVTA AFE G +QS D T
Sbjct 181 TIAG--NDDGDAWKALNSDPNKPMDNRALKYTYPPGSTFKLVTATAAFENGMFQSIDDTT 238
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TP+ YILPGT TPL N SP E C NATV+ +D SCNTVF K+GA+LG DK+RAQAEKF
Sbjct 239 STPEPYILPGTQTPLKNESPNERCENATVKSGLDQSCNTVFAKIGADLGKDKMRAQAEKF 298
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFN + P+RA+ S FP+ + DGTA D+IGQHDTRATPLQ+AMV+AAIANNGSLM+PY
Sbjct 299 GFNSTIDTPVRAEKSVFPNSASLDGTAQDSIGQHDTRATPLQVAMVSAAIANNGSLMQPY 358
Query 1081 LVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
LV QE SA IS HTE Q SQA+SPATAQK+Q LM SVVQ GTG NA+IPGV VGGKT
Sbjct 359 LVDQERSASLTTISKHTEKQFSQAMSPATAQKMQDLMVSVVQDGTGKNAQIPGVQVGGKT 418
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHG++NS LP+AWF S+AK DG+ VAVAVVVEDGS ISGG LAAPIAKA+MQ
Sbjct 419 GTAQHGENNSALPYAWFTSWAKTPDGQSVAVAVVVEDGSDNRDGISGGRLAAPIAKAVMQ 478
Query 1438 AALGK 1452
AALGK
Sbjct 479 AALGK 483
>WP_033346841.1 penicillin-binding protein 2 [Kitasatospora aureofaciens]
Length=483
Score = 724 bits (1870), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 354/485 (73%), Positives = 400/485 (82%), Gaps = 3/485 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVSIFCLVLILALM+R NWVQGVQA WA+N +N R YD+YA+PRGNIIV GQ
Sbjct 1 MNKPIRRVSIFCLVLILALMVRTNWVQGVQADAWASNKNNKRQLYDRYAHPRGNIIVSGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT SDFVNGLRYKYKRSW++GPMYAPVTGYSSQ Y ++ LE LEDGILSGTD RLFFRN
Sbjct 61 PVTNSDFVNGLRYKYKRSWINGPMYAPVTGYSSQAYGSNGLENLEDGILSGTDDRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTG+ ++GGDVVTTI+ KVQKA F+ +G+KKGA VA+DPKTGAIL LVSTPSYDPG
Sbjct 121 TLDMLTGEQQRGGDVVTTINAKVQKAAFDAMGDKKGAVVALDPKTGAILALVSTPSYDPG 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
T AG DD AW AL+SDPNKPM NRAL+ TYPPGSTFKLVTA AFE G +QS D T
Sbjct 181 TIAG--NDDGDAWKALNSDPNKPMDNRALKYTYPPGSTFKLVTATAAFENGMFQSIDDTT 238
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
TP+ YILPGT TPL N SP E C NATV+ +D SCNTVF K+GA+LG DK+RAQAEKF
Sbjct 239 STPEPYILPGTQTPLKNESPNERCENATVKSGLDQSCNTVFAKIGADLGKDKMRAQAEKF 298
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFN + P+RA+ S FP+ + DGTA D+IGQHDTRATPLQ+AMV+AAIANNGSLM+PY
Sbjct 299 GFNSTIDTPVRAEKSVFPNSASLDGTAQDSIGQHDTRATPLQVAMVSAAIANNGSLMQPY 358
Query 1081 LVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
LV QE SA +S HTE Q SQA+SPATAQK+Q LM SVVQ GTG NA+IPGV VGGKT
Sbjct 359 LVDQERSASLTTVSKHTEKQFSQAMSPATAQKMQDLMVSVVQDGTGKNAQIPGVQVGGKT 418
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHG++NS LP+AWF S+AK DG+ VAVAVVVEDGS ISGG LAAPIAKA+MQ
Sbjct 419 GTAQHGENNSALPYAWFTSWAKTPDGQSVAVAVVVEDGSDNRDGISGGRLAAPIAKAVMQ 478
Query 1438 AALGK 1452
AALGK
Sbjct 479 AALGK 483
>WP_035799332.1 penicillin-binding protein [Kitasatospora mediocidica]
Length=483
Score = 712 bits (1839), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 356/485 (73%), Positives = 411/485 (85%), Gaps = 3/485 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVS+FCL+LI+AL+LR NW+QGVQA ++AN+P+NDR KYD+YAYPRGNIIVGGQ
Sbjct 1 MNKPIRRVSVFCLILIMALLLRTNWIQGVQADSYANSPYNDRVKYDRYAYPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT+S+FVNG RYKYK W DG MYAPVTGYSSQ Y ++QLE+LED ILSGTD RLFFRN
Sbjct 61 PVTQSNFVNGYRYKYKTGWTDGAMYAPVTGYSSQAYGSNQLEQLEDPILSGTDDRLFFRN 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TLDMLTGK KQGGDVVTTI+ K QKA F+GLG+KKGAAVAIDP+TGAIL LVSTPSYDP
Sbjct 121 TLDMLTGKQKQGGDVVTTINAKAQKAAFDGLGDKKGAAVAIDPRTGAILALVSTPSYDPS 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
T AG T D+ AW +L++DPN+PMLNRALR+TYPPGSTFKLVTAATAFETGK+QSP D T
Sbjct 181 TIAGTT--DDTAWKSLNADPNQPMLNRALRQTYPPGSTFKLVTAATAFETGKFQSPDDQT 238
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
DTP Y LPGT+T L N SP E C NAT++ A+ +SCNTVFGK+GAELG KL AQA+KF
Sbjct 239 DTPQTYTLPGTNTQLNNDSPDEPCTNATLKDALAISCNTVFGKIGAELGSQKLLAQADKF 298
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFN V PIR+ S FP+ S PDGTAMDAIGQH+T ATPLQMAMVAAAIANNG LM+PY
Sbjct 299 GFNNTVDTPIRSAPSVFPNNSSPDGTAMDAIGQHNTAATPLQMAMVAAAIANNGKLMQPY 358
Query 1081 LVAQEGSAGN-VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
LV +E SA + IS HTEH++ QA+SPATAQKLQQ+M +VVQ GTG A+I GV VGGKT
Sbjct 359 LVDEERSANSTTISKHTEHEMDQAISPATAQKLQQMMVAVVQDGTGKKAQINGVEVGGKT 418
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHGQ NSGLPFAWFVSYAK D VAVAVVVEDG++ + +ISGGS+AAPIAK++MQ
Sbjct 419 GTAQHGQGNSGLPFAWFVSYAKVGDNAPVAVAVVVEDGAASSNEISGGSIAAPIAKSVMQ 478
Query 1438 AALGK 1452
A +G+
Sbjct 479 AVIGQ 483
>WP_035847202.1 penicillin-binding protein [Kitasatospora azatica]
Length=486
Score = 704 bits (1817), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 342/486 (70%), Positives = 404/486 (83%), Gaps = 2/486 (0%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKPIRRVS+FC+VLI AL+LR NWVQGV A ++A NP+N RT YD+Y+YPRGNII GGQ
Sbjct 1 MNKPIRRVSVFCMVLIFALLLRTNWVQGVVADSYAENPNNRRTVYDQYSYPRGNIIAGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQT-YDASQLEKLEDGILSGTDSRLFFR 357
+T+SDF NG YKYKR + DG MYAPVTG++SQ+ + ++ LE LE+G+L+GTD RLFFR
Sbjct 61 PITRSDFTNGYLYKYKRGYTDGAMYAPVTGFTSQSAFGSNGLENLENGVLAGTDDRLFFR 120
Query 358 NTLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDP 537
N+LD LTGK ++GGDVVTTI+PKVQKA F+ L KKGAAVA+DP+TGAIL LVSTPSYDP
Sbjct 121 NSLDALTGKQRKGGDVVTTINPKVQKAAFDALSGKKGAAVALDPRTGAILALVSTPSYDP 180
Query 538 GTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDV 717
T AG + D AW AL++D N+PML+RALR+TYPPGSTFKLVTAATAFETGKYQ+P D
Sbjct 181 NTIAGTSGADGDAWKALNADQNQPMLDRALRQTYPPGSTFKLVTAATAFETGKYQNPDDQ 240
Query 718 TDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEK 897
TDTPDQYILPGTST L N S EDCG AT++HA+ +SCNTV+GK+GA+LG +KL+AQAEK
Sbjct 241 TDTPDQYILPGTSTQLHNDSDDEDCGQATLKHALTISCNTVYGKLGADLGKEKLQAQAEK 300
Query 898 FGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
FGFN V IPIR+ S +P+ S PDGTAMDAIGQH+T ATPLQMAMVAAAIA+NG LM+P
Sbjct 301 FGFNSTVDIPIRSAKSVYPTDSTPDGTAMDAIGQHNTAATPLQMAMVAAAIADNGKLMQP 360
Query 1078 YLVAQEGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
YLV +E SA G +S H E QL QAVSPATAQKLQQ+M VVQ+GTG NA+I GV VGGK
Sbjct 361 YLVDEERSAGGTTLSKHAEKQLGQAVSPATAQKLQQMMVDVVQNGTGRNARIDGVEVGGK 420
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMM 1434
TGTAQHG DN GLPFAWFVSYAK VAVAVVVEDGS++ +ISGG LAAP+AK +M
Sbjct 421 TGTAQHGTDNKGLPFAWFVSYAKVGADTPVAVAVVVEDGSAQRGEISGGGLAAPVAKLIM 480
Query 1435 QAALGK 1452
QAA+G+
Sbjct 481 QAAIGR 486
>WP_084747517.1 penicillin-binding protein [Kitasatospora albolonga]
ARF73734.1 penicillin-binding protein [Kitasatospora albolonga]
Length=485
Score = 548 bits (1411), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 272/483 (56%), Positives = 349/483 (72%), Gaps = 3/483 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKP+RRV+IFC +L+L+L+LR NW+Q +A N N R + ++YA+ RGNIIV G+
Sbjct 1 MNKPLRRVAIFCGILMLSLLLRDNWIQYARADELTNRSENRRIRIERYAHERGNIIVDGK 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVT S NG ++YKR W DGP++APVTGYSSQ +D+SQLE +EDGIL+G D +LFF
Sbjct 61 AVTGSVETNGSDFRYKRVWKDGPLWAPVTGYSSQAFDSSQLENIEDGILTGNDDQLFFNR 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
TL M TG+ KQGG++VTT++ QKA F+GLG KKGA VA+DP+TGAIL L STPSYDP
Sbjct 121 TLSMFTGQKKQGGNIVTTLNGAAQKAAFKGLGQKKGAVVALDPQTGAILALASTPSYDPS 180
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
FAG + D +A L D +KPMLNRALRETYPPGSTFK+VTAA A E G Y T
Sbjct 181 VFAGNSTKDSEARQKLLDDKDKPMLNRALRETYPPGSTFKVVTAAAALENGLYDDIDAKT 240
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
++P + LP T L N C +A+++ A+ +SCNTVFGK+ +LG K+ Q +KF
Sbjct 241 ESPLPWTLPQTKVALQNQGNLP-CEDASLREALRVSCNTVFGKVSDDLGNKKMIEQTDKF 299
Query 901 GFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPY 1080
GFNKEV P+RADAS +P ++P AM IGQ R TPLQMAMV AAIAN+G LM+PY
Sbjct 300 GFNKEVFTPVRADASIYPEDNRPQ-NAMAGIGQASNRTTPLQMAMVTAAIANDGKLMKPY 358
Query 1081 LVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKT 1257
+VA+ + + + TH +L +++S AQKLQQ+ME+VV GTGT A++PGVTVGGKT
Sbjct 359 MVAKRQTPDLDDVYTHQPEELGRSLSGENAQKLQQMMETVVNDGTGTRAQLPGVTVGGKT 418
Query 1258 GTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQ 1437
GTAQHG +NS P+AWF+SYAK +G VAVAVVVEDG++ ISGG LAAPIA+ +M+
Sbjct 419 GTAQHGLNNSEKPYAWFISYAKTDNGSPVAVAVVVEDGNANRGDISGGGLAAPIARDVMK 478
Query 1438 AAL 1446
A +
Sbjct 479 AVI 481
>WP_003978842.1 MULTISPECIES: penicillin-binding protein [Streptomycetaceae]
KOG83606.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=489
Score = 541 bits (1394), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 269/486 (55%), Positives = 355/486 (73%), Gaps = 5/486 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKP+RRV+IFC +L+L+L++RVNWVQ VQA N+ +N R ++Y+ PRGNIIVGGQ
Sbjct 1 MNKPLRRVAIFCGLLVLSLLIRVNWVQFVQADELKNDTNNRRVAIERYSQPRGNIIVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
A+T S + +KYKR++ DGPM+APVTGY+SQ + ++QLEKL D ILSGTD +LFF
Sbjct 61 AITGSTTNDSGDFKYKRTYKDGPMWAPVTGYASQAFGSNQLEKLNDSILSGTDDKLFFSR 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
T+DM TG+ ++GGDVVTT++PK QKA ++GLG KGA AI+P+TGAIL L STPSYDP
Sbjct 121 TVDMFTGEKQKGGDVVTTLNPKAQKAAYDGLGKSKGAVAAINPETGAILALASTPSYDPS 180
Query 541 TFAGGTKDDEKAWTAL--DSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSD 714
TFAG + D KA+ AL ++P+ PMLNRALR+TYPPGSTFK+VTAA A E G Y +
Sbjct 181 TFAGMSDKDAKAYNALLKKNNPDDPMLNRALRQTYPPGSTFKVVTAAAALENGLYTDINA 240
Query 715 VTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAE 894
TD+P + LP T+ +N C NA+++ A+ SCNTVFGK+ A+LG K++A+AE
Sbjct 241 KTDSPLPWYLPDTAHQPLNNEGNIPCENASLKEALRWSCNTVFGKISADLGNKKMKAEAE 300
Query 895 KFGFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLM 1071
KFG+N ++ P+RA S +P+ ++P AM IGQ RATPLQMAM+ +AIAN G LM
Sbjct 301 KFGYNDAKIDTPVRAAESVYPTDNRPQ-NAMAGIGQASNRATPLQMAMITSAIANGGKLM 359
Query 1072 EPYLVAQEGSAG-NVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVG 1248
+PY+V + + NV+ HT +SQ + P AQKLQ++ME VV++GTGTN +I GVTVG
Sbjct 360 KPYMVDKLVAPNLNVVQQHTPEAMSQPLKPENAQKLQEMMEGVVKNGTGTNGQIDGVTVG 419
Query 1249 GKTGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKA 1428
GKTGTAQHG++N P+AWF+SYAK G VAVAVV+E + I+GG LAAPIAK
Sbjct 420 GKTGTAQHGENNKDNPYAWFISYAKTSKGTPVAVAVVIEGSDTLRGDIAGGKLAAPIAKK 479
Query 1429 MMQAAL 1446
+M+A L
Sbjct 480 VMEAVL 485
>WP_052836711.1 penicillin-binding protein 2 [Kitasatospora aureofaciens]
Length=490
Score = 516 bits (1329), Expect = 1e-180, Method: Compositional matrix adjust.
Identities = 263/488 (54%), Positives = 345/488 (71%), Gaps = 8/488 (2%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKP+RR++IFC +L+L L++R NW+Q VQA + HN R ++YA PRG+IIV G+
Sbjct 1 MNKPLRRIAIFCGLLVLTLLIRDNWLQYVQADELKEDEHNRRVAIERYASPRGDIIVDGK 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
A+T G +KYKR++ DG M+APVTGY SQ + A+QLE ++DGIL+G D RLFFRN
Sbjct 61 AITGHATTEG-DFKYKRTYKDGAMWAPVTGYVSQAFGATQLESIDDGILTGNDDRLFFRN 119
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNK--KGAAVAIDPKTGAILGLVSTPSYD 534
TLDM+TG+ ++GG+VVTT++ QKA + GL + KGA VAI+P TG IL + S PSYD
Sbjct 120 TLDMITGEKREGGNVVTTLNSAAQKAAYNGLKKQGAKGAVVAIEPSTGKILSMASYPSYD 179
Query 535 PGTFAGGTKDDEKAWTALD--SDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSP 708
P + AG +AW L ++P+ PMLNRALRE YPPGSTFK+VTAA A E GKY S
Sbjct 180 PTSIAGSNDAAGEAWNRLQKKNNPDDPMLNRALREAYPPGSTFKVVTAAAALEHGKYTSA 239
Query 709 SDVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQ 888
+ TD+P + +PGT+TPL N C NAT++ A+ +SCNTVFGK+G +LG DK+
Sbjct 240 DEKTDSPLPWTMPGTTTPLPNEGDLP-CENATLREALRVSCNTVFGKIGYDLGNDKMLET 298
Query 889 AEKFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSL 1068
A+KFGF KE +P+R+ AS F A+ +IGQ +T ATPLQMAMV +AIANNG+L
Sbjct 299 AKKFGFTKEQFVPVRSSASVFSDDMNDSQVALSSIGQFNTAATPLQMAMVTSAIANNGTL 358
Query 1069 MEPYLV-AQEGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTV 1245
M+PY+V A + + I ++S+ +S AQ LQ +ME+VV+ GTGTNA+I GVTV
Sbjct 359 MKPYMVDALQAPNLDTIEKTDPEEMSKPLSADNAQILQSMMETVVKDGTGTNAQIDGVTV 418
Query 1246 GGKTGTAQHGQDNSGLPFAWFVSYAKGQDGKQ-VAVAVVVEDGSSEAQQISGGSLAAPIA 1422
GGKTGTAQHG DNS P+AWF+SYAK DG VAVAVV+ED ++ ISGG LAAPIA
Sbjct 419 GGKTGTAQHGVDNSENPYAWFISYAKTDDGSSPVAVAVVIEDENASRDDISGGGLAAPIA 478
Query 1423 KAMMQAAL 1446
K +M+A +
Sbjct 479 KNVMEAVI 486
>WP_003985178.1 MULTISPECIES: penicillin-binding protein [Streptomycetaceae]
KOG71939.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=482
Score = 514 bits (1323), Expect = 1e-179, Method: Compositional matrix adjust.
Identities = 273/484 (56%), Positives = 344/484 (71%), Gaps = 5/484 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKP+RRVS+FC++LILAL+ V W+QG +A+T++++PHN R KYA P GNI+VGGQ
Sbjct 1 MNKPLRRVSVFCILLILALLAWVTWLQGAKAATFSDDPHNPRVSIAKYANPLGNILVGGQ 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT S +G KY+R++ +GP+YAPVTG+SSQ + ++QLE L +L G+DSRL +N
Sbjct 61 PVTGSTATDGT-LKYQRTYKNGPLYAPVTGFSSQIFGSNQLESLYGDLLDGSDSRL--QN 117
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
D LT + +GGDV TTID KVQ+AG++ LG K GAAVA+DP TG ILG+VSTPSYDPG
Sbjct 118 PGDALTRQRPKGGDVATTIDAKVQQAGYKALGGKSGAAVALDPATGKILGMVSTPSYDPG 177
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
FAG D+KAW L + P +NRALR+ PPGSTFKLV AA A E G Y S + T
Sbjct 178 KFAGSGSGDQKAWKDLSGNKAHPEINRALRQPLPPGSTFKLVVAAAALENGLYSSVDEAT 237
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
D+PD Y LP T T L N S + C NAT++ A+ SCNTVF KM A+LG DK+RAQAEKF
Sbjct 238 DSPDPYTLPNTRTVLKNESASAPCKNATIRTALQYSCNTVFAKMAADLGQDKVRAQAEKF 297
Query 901 GFNK-EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN ++ P+RA S +P G TA+ IGQ D ATPLQMAMV++AIAN G L P
Sbjct 298 GFNNGKLDTPVRAGKSLYPQGMDKPQTALSGIGQFDDTATPLQMAMVSSAIANGGDLQTP 357
Query 1078 YLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
+V + GN + + + +AVS TA++LQ ME+VV GTG+NAKI G+TVGGK
Sbjct 358 QMVDKLTDGGGNTLQEYEPKKYGKAVSRRTAEQLQSSMETVVNEGTGSNAKIDGLTVGGK 417
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMM 1434
TGTAQHG +N G P+AWFVSYAK G QVAVAV+VED + +ISGG LAAP+AKAMM
Sbjct 418 TGTAQHGVNNEGTPYAWFVSYAKDGKGHQVAVAVMVEDSDAARSEISGGGLAAPVAKAMM 477
Query 1435 QAAL 1446
+AAL
Sbjct 478 KAAL 481
>WP_052841852.1 penicillin-binding protein 2 [Kitasatospora aureofaciens]
Length=484
Score = 491 bits (1265), Expect = 7e-171, Method: Compositional matrix adjust.
Identities = 256/485 (53%), Positives = 327/485 (67%), Gaps = 6/485 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NK IRR S+F L+L+ AL++R WVQ + A++ N R + YA P GNIIV G+
Sbjct 1 MNKTIRRASVFTLLLVFALLIRATWVQFYEGRALADSKDNRRGAIETYAEPLGNIIVAGR 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
+VT S G YKR++ +G +YA VTGY+SQ Y +QLE + +L+GTD RL R
Sbjct 61 SVTGSAPTEGGDLAYKRTYENGKLYAAVTGYASQAYAPTQLEGIYQDVLNGTDPRL--RT 118
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
+D +TG + G+VVTTIDP VQKAGFE LG+ KGAAVAIDPKTG IL +VSTPSYDP
Sbjct 119 VMDTVTGTRAEPGNVVTTIDPAVQKAGFEALGDTKGAAVAIDPKTGRILSVVSTPSYDPT 178
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
+ E AW L DP+KP+ NRALR+ PPGSTFKLV AA A E G Y S + T
Sbjct 179 SLTDANTAGE-AWKKLTGDPDKPLTNRALRQPLPPGSTFKLVVAAAALEDGLYGSVDERT 237
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
D+PD Y LPGT+ L N +P C NAT++ A+ SCN VF KM +LG D+LRA AEKF
Sbjct 238 DSPDPYTLPGTTRDLSNENPDAPCENATIRTALQYSCNNVFAKMAVDLGQDELRAMAEKF 297
Query 901 GFNKEVT-IPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN E +P+RA S +P TA+ IGQ D ATPLQMAMV+AA+AN G L+ P
Sbjct 298 GFNDETQDVPVRAYTSVYPKDMNRSSTALTGIGQFDVTATPLQMAMVSAALANGGELVSP 357
Query 1078 YLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
++V+Q S G+ + HT+ + VS ATA++L+ M +VV+ GTG+NA+I G TVGGK
Sbjct 358 HMVSQVTDSGGDALEDHTDAGSKRIVSSATAEQLRSAMRTVVEKGTGSNAQIAGATVGGK 417
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDG-KQVAVAVVVEDGSSEAQQISGGSLAAPIAKAM 1431
TGTAQHG++NS P+AWF SYAK K+VAVAV+VE + ++SG LAAP+AKAM
Sbjct 418 TGTAQHGENNSKTPYAWFTSYAKSDSSDKEVAVAVIVEQSDAARSEVSGNGLAAPVAKAM 477
Query 1432 MQAAL 1446
M+AAL
Sbjct 478 MEAAL 482
>WP_084745269.1 penicillin-binding protein [Kitasatospora albolonga]
ARF71899.1 penicillin-binding protein [Kitasatospora albolonga]
Length=483
Score = 481 bits (1238), Expect = 7e-167, Method: Compositional matrix adjust.
Identities = 251/487 (52%), Positives = 325/487 (67%), Gaps = 7/487 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NK IRR S FCL+++LAL++R WVQG +A A+N HN R +YA+P G+IIV G
Sbjct 1 MNKTIRRASAFCLLMVLALLVRATWVQGYEAKALADNEHNRRNTIAQYAHPLGDIIVAGS 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT S +G +YKR++ G +YA VTGYSSQ Y A+QLE + G+L GTD RL +N
Sbjct 61 PVTGSKGTSGGDLRYKRTYTQGELYAAVTGYSSQAYGATQLEGIYSGVLDGTDDRL--KN 118
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
D++TG+ G+V+TTIDP VQKA +E LG KGAAVAIDPKTG ILG+VSTPSYDP
Sbjct 119 PADLITGRQAVPGNVLTTIDPGVQKAAYEALGGDKGAAVAIDPKTGRILGMVSTPSYDPS 178
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
+G D AW L D +KP++NRALR+ PGSTFKLV AA A E G Y S + T
Sbjct 179 EISG--TGDGDAWRKLLDDKDKPLVNRALRQPLAPGSTFKLVVAAAALENGLYGSVDEAT 236
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
D+PD Y LPGT T L N + + C NAT++ A+ SCN VF K A+LG DK++ A+ F
Sbjct 237 DSPDPYTLPGTRTILRNENASAPCENATLRTALQYSCNNVFAKAAADLGQDKVKEMADAF 296
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GF+ E + +P+RA S +P+G TA+ IGQ + ATPLQMAMV AA+AN G L P
Sbjct 297 GFDTEKLDVPVRASKSVYPTGMDKAQTALTGIGQFEVTATPLQMAMVTAALANGGELAAP 356
Query 1078 YLVAQEGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
++V+ G+ + + + V TA++L+ M +VV+ GTGTNA+I G VGGK
Sbjct 357 HMVSSVTDGDGSALEKTEDGDTKRVVDEKTAEQLRSAMVTVVEEGTGTNARIAGAEVGGK 416
Query 1255 TGTAQHGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAM 1431
TGTAQ+G DNS P+AWF SYAK GK+VAVAV+VED S ++SG LAAPIA+ M
Sbjct 417 TGTAQNGVDNSNTPYAWFTSYAKDDSSGKEVAVAVIVEDSGSARSEVSGNGLAAPIAQKM 476
Query 1432 MQAALGK 1452
M+AAL +
Sbjct 477 MKAALER 483
>WP_051969980.1 penicillin-binding protein [Kitasatospora azatica]
Length=497
Score = 461 bits (1185), Expect = 1e-158, Method: Compositional matrix adjust.
Identities = 253/483 (52%), Positives = 321/483 (66%), Gaps = 8/483 (2%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FCLVL +AL + VQ A T+ +NP N RT +YA PRGN++VG VT S
Sbjct 15 RRAGTFCLVLTVALAAQATRVQFFNAETYNDNPANQRTTIQRYAQPRGNLLVGADPVTGS 74
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY YKR++ +GP YA VTG+SSQ + +QLE ED +L+GTD+RL D +
Sbjct 75 TATGG-RYDYKRTYTEGPQYAAVTGFSSQIFGNTQLEGTEDALLTGTDNRLAGWALWDAI 133
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ K GGDV TTI Q+A +GLGN+KGA AI+P TG IL L STPSYDPG+FAG
Sbjct 134 SRKQSPGGDVYTTISKAAQQAAVQGLGNQKGAVAAIEPGTGRILALASTPSYDPGSFAGS 193
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
D++AW L +D N+PMLNRALR+TYPPGSTFK+VTAA A G + T P
Sbjct 194 AAADQEAWNKLQADSNQPMLNRALRQTYPPGSTFKVVTAAAALANGVVTDINAPTGAPYP 253
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L+N + + D N + AM LSCN+V G +G + G DK+ + A+KFGFN
Sbjct 254 YVLPGTTTELVNDTSSCDQPNLDLDTAMTLSCNSVLGYLGVQTGLDKMVSMAQKFGFNDA 313
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ IP+RA S+F + A+ +IGQ DT ATPL MAMVAA +ANNG++M P LV +
Sbjct 314 RLDIPVRAARSNFDTSMNQSQVALSSIGQFDTAATPLVMAMVAAGVANNGTVMYPQLVDK 373
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G+ I H QA+SPA A ++QQLM VVQ+GTG NA+I G TVGGKTGTAQ
Sbjct 374 LTKSDGSTIQLMKPHPYQQAMSPAVAGQVQQLMTDVVQNGTGRNARIAGATVGGKTGTAQ 433
Query 1270 HGQDNSGLPFAWFVSYAK---GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA 1440
HG DNSG+P+AWF+S+A+ D VAVAVV+ D SEA ++GG LAAPIAK++MQA
Sbjct 434 HGVDNSGVPYAWFISWARPTGSTDVPPVAVAVVIAD--SEATDVTGGGLAAPIAKSVMQA 491
Query 1441 ALG 1449
LG
Sbjct 492 VLG 494
>WP_030393477.1 MULTISPECIES: penicillin-binding protein [Kitasatospora]
Length=486
Score = 447 bits (1151), Expect = 1e-153, Method: Compositional matrix adjust.
Identities = 240/482 (50%), Positives = 315/482 (65%), Gaps = 4/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FCL+L +AL ++ VQ +A +N N R ++Y+ PRG+I VG +VT S
Sbjct 6 RRAGTFCLLLTVALCVQATRVQVFRADELNHNSANQRLVVERYSQPRGSIRVGADSVTGS 65
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
+ G RY YKR++ DGP+YA VTGYSSQTY SQLE ED +LSGTDSRL D +
Sbjct 66 EPTGG-RYDYKRTYTDGPLYAAVTGYSSQTYGNSQLEGTEDALLSGTDSRLASWAVWDAV 124
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ GGDV TT+D VQ+A GLG +KGA A++P TG IL L STPSYDPG+FAG
Sbjct 125 ARRQNPGGDVRTTVDRAVQEAAVRGLGRQKGAVAAVEPATGRILALASTPSYDPGSFAGT 184
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ DD +AW+ L SDP++PMLNRALR+TYPPGSTFK+VTAA A E+G TDTP+
Sbjct 185 SADDREAWSRLQSDPDRPMLNRALRQTYPPGSTFKVVTAAAALESGVVTDVDAPTDTPNP 244
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGTS PL+N S D N ++ AM LSCN+V G++G E+G ++ A AE FGFN+
Sbjct 245 YLLPGTSVPLVNDSAACDEPNQSLATAMALSCNSVLGRLGVEVGLRRMAATAEGFGFNEA 304
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ P+RA S+F + A+ +IGQ DT ATPL MAMVAA IANNG++M P LV
Sbjct 305 RLDTPVRAARSNFDTRMDEAQLALSSIGQFDTAATPLVMAMVAAGIANNGTVMRPQLVDS 364
Query 1093 EGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
+A G V+ + QAV P TA ++++LM V++GTG A+I G VGGKTGTAQ
Sbjct 365 LTAADGTVVHSVRPSVYRQAVGPGTAGQVRRLMVDAVENGTGRAARIDGAVVGGKTGTAQ 424
Query 1270 HGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQ-QISGGSLAAPIAKAMMQAAL 1446
HG DN+G P+AWF+++A V V + +++GG LAAP+A+A+MQA L
Sbjct 425 HGVDNTGTPYAWFIAWAAPAGSAAVPPVAVAVVVADSDATEVTGGGLAAPVARAVMQAVL 484
Query 1447 GK 1452
+
Sbjct 485 SR 486
>WP_051966736.1 penicillin-binding protein [Kitasatospora mediocidica]
Length=499
Score = 446 bits (1146), Expect = 1e-152, Method: Compositional matrix adjust.
Identities = 250/480 (52%), Positives = 312/480 (65%), Gaps = 4/480 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR +FCLVLILAL + WVQ QA + +N N RT ++YA PRGNI+VG VT S
Sbjct 18 RRAGMFCLVLILALAGQATWVQVFQAKAYDDNAANQRTAIERYAQPRGNILVGAAPVTGS 77
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G R+ YKR++ DG +A VTG+SSQ Y +QLE ED +LSGTD+RL D +
Sbjct 78 APTGG-RFDYKRTYTDGSQFAAVTGFSSQIYGNTQLEGTEDSLLSGTDNRLAGWALWDEI 136
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ K GGDV TTI Q+A +GLGN+KGA AI+P TG IL L STPSYDPG+FAG
Sbjct 137 SRKQNPGGDVYTTISKAAQQAAMQGLGNQKGAVAAIEPSTGRILALASTPSYDPGSFAGS 196
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
T D+ AW AL +D N+PMLNRALR+TYPPGSTFK+VTAA A G + T P
Sbjct 197 TAADQNAWNALQADTNQPMLNRALRQTYPPGSTFKVVTAAAALANGTVTDINAPTGAPSP 256
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L+N + D ++ AM LSCN+V G +G + G DK+ A AE FGFN
Sbjct 257 YVLPGTTTTLVNDTTACDRSGLSLDTAMVLSCNSVLGYLGVQTGLDKMTAMAENFGFNDA 316
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
++ IP+RA S+F + A+ +IGQ DT ATPL MAMVAA +ANNG++M P LV +
Sbjct 317 KLDIPVRAARSNFDTRMNQSQVALSSIGQFDTAATPLVMAMVAAGVANNGTVMYPQLVDK 376
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + QA+SPA A ++QQLM VV +GTG NA+I G TVGGKTGTAQ
Sbjct 377 LTKSDGTTVQVMKPSTYKQAMSPAVAGQVQQLMTDVVDNGTGRNARISGATVGGKTGTAQ 436
Query 1270 HGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG DNSG P+AWF+S+AK VAV V S A ++GG LAAPIAK++MQA L
Sbjct 437 HGVDNSGTPYAWFISWAKPAGSSAVPPVAVAVVIADSNATDVTGGGLAAPIAKSVMQAVL 496
>AUG79326.1 Putative penicillin-binding protein A [Kitasatospora sp. MMS16-BH015]
Length=516
Score = 445 bits (1144), Expect = 4e-152, Method: Compositional matrix adjust.
Identities = 240/482 (50%), Positives = 312/482 (65%), Gaps = 4/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FC++LI+AL ++ +Q QA + +N N R ++Y PRGNI+VG VT S
Sbjct 36 RRAGTFCMLLIVALAVQATRIQVFQADSLDHNSANQRLTIERYGQPRGNILVGADPVTGS 95
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY YKR++ DGP YA VTG+SSQTY +QLE ED +LSGTD+RL D +
Sbjct 96 APTGG-RYDYKRTYTDGPQYAAVTGFSSQTYGNTQLEGTEDELLSGTDTRLAGWALWDEI 154
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
K GGDV TTID Q+A +GLG++KGA AI+P TG IL L STPSYDPG+FAG
Sbjct 155 ARKQNPGGDVFTTIDKAAQQAAMKGLGDQKGAVAAIEPATGRILALASTPSYDPGSFAGA 214
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
K D++AW L +D N+PMLNRALR+ YPPGSTFK+VTA A G T P
Sbjct 215 GKTDQQAWDRLQADTNQPMLNRALRQIYPPGSTFKVVTATAALANGVVTDIDKGTGAPYP 274
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y++PGT+TPL+N + D N T+ AM SCN+V G +G ++G DK+ A A+ FGFN
Sbjct 275 YVMPGTTTPLVNDTTACDEPNQTLDQAMVSSCNSVMGYLGVQVGLDKMVAMAQSFGFNDP 334
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ IP+RA S+F + A+ +IGQ DT ATPL MAMVAA +ANNG++M P LV +
Sbjct 335 RLDIPVRAARSNFDTKMNQSQVALSSIGQFDTAATPLVMAMVAAGVANNGTVMHPQLVDK 394
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G+ + QA+SPA A+++QQLM VV+ GTG +A+I G TVGGKTGTAQ
Sbjct 395 LTKSDGSSVQVMKPRTYRQAMSPAVARQVQQLMTDVVESGTGCSARIGGATVGGKTGTAQ 454
Query 1270 HGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVED-GSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG DNSG+P+AWF+S+AK QV V S A ++GG LAAPIA+++MQ+ L
Sbjct 455 HGVDNSGIPYAWFISWAKPSGSDQVPPVAVAVVIADSGATDVTGGGLAAPIARSVMQSIL 514
Query 1447 GK 1452
+
Sbjct 515 DR 516
>AUG79205.1 penicillin-binding protein [Kitasatospora sp. MMS16-BH015]
Length=492
Score = 441 bits (1135), Expect = 3e-151, Method: Compositional matrix adjust.
Identities = 245/486 (50%), Positives = 318/486 (65%), Gaps = 5/486 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+ + IRR S+FCL+L+LALM+RV W+ +A ++ NP N R D+YA P GNI+VGGQ
Sbjct 10 MTRSIRRASLFCLLLVLALMVRVTWIAVAKADSYDKNPANARNTIDRYADPLGNILVGGQ 69
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT S G Y+R++ DGP+YAPVTGYSSQ Y ++QLE L +L G+D RL +
Sbjct 70 PVTGSK-AGGGSLAYQRTYADGPLYAPVTGYSSQAYGSTQLENLYGPLLDGSDPRL--AS 126
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
LT GGDVVT+IDP VQ+A + LG K+GAAVAIDP TG IL LVSTPS+DP
Sbjct 127 PFAALTRDRPAGGDVVTSIDPAVQQAAWNALGGKQGAAVAIDPDTGRILALVSTPSWDPN 186
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
G + D AW L +D MLN ALR TY PGSTFKLVT A A E G Y T
Sbjct 187 RITGRSAADAAAWKQLSADNGSAMLNSALRSTYAPGSTFKLVTLAAALENGLYSDVDQPT 246
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
D+PD Y LPGT T L N +P+ C NA+++ A++ SCN VF K+ A+LG K+ AQA KF
Sbjct 247 DSPDPYTLPGTRTVLTNENPSAPCTNASLRTALEYSCNNVFAKVAADLGETKMAAQAAKF 306
Query 901 GFN-KEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GF ++ +P+RA S +PSG + A+ IGQ ATPLQMAMV+AA+AN G LM+P
Sbjct 307 GFGLGQLDVPVRAAESVYPSGMEKSQVALTGIGQFSVTATPLQMAMVSAAVANGGVLMKP 366
Query 1078 YLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
+V + +G + +L Q +S TA ++ M SVV GTG A + G+TVGGK
Sbjct 367 SMVEKLTDHSGRTVQEFGPARLDQVMSKRTADQIHSAMVSVVTAGTGRAAAVKGLTVGGK 426
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMM 1434
TGTAQ+G DNSG+P+AWF S+A+ GK++A AVVV DGS++ ++GG LA P++KAM
Sbjct 427 TGTAQNGIDNSGIPYAWFTSFAEDGSGKRIATAVVVLDGSADRDAVTGGGLAGPVSKAMA 486
Query 1435 QAALGK 1452
QA L +
Sbjct 487 QAYLKR 492
>WP_084746985.1 penicillin-binding protein 2 [Kitasatospora albolonga]
ARF73187.1 penicillin-binding protein [Kitasatospora albolonga]
Length=494
Score = 440 bits (1132), Expect = 1e-150, Method: Compositional matrix adjust.
Identities = 235/494 (48%), Positives = 325/494 (66%), Gaps = 10/494 (2%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+N+P+R ++IFC +L+LAL+LR NW+Q V + + N R +++++A PRG+IIVGG+
Sbjct 1 MNRPLRHIAIFCGLLMLALLLRANWLQQVDRVELSQHERNVRVRFERFASPRGDIIVGGK 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
A+T S + Y ++R+W DGPMYAPVTGY+SQ S LE+ D +LSG D R FR+
Sbjct 61 AITGSKETDSRDYTFRRTWKDGPMYAPVTGYASQAQGTSLLERTYDSVLSGQDDRFAFRH 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGN--KKGAAVAIDPKTGAILGLVSTPSYD 534
D+LTG+P++GG VVTTID K QKAG++GL + +G+ VA+DP+TG +L LVSTPSYD
Sbjct 121 AKDVLTGQPRRGGSVVTTIDAKAQKAGYKGLTDLGARGSVVALDPRTGRVLALVSTPSYD 180
Query 535 PGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSD 714
P FAG + + + AL D NKP+ NRALRET+PPGSTFK++TAA A E G
Sbjct 181 PEVFAGISFKESDRFKALLEDKNKPLANRALRETFPPGSTFKILTAAAALEHGVITDVDA 240
Query 715 VTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAE 894
T Y LP ++ + + + CG A+++ AM SCN VF + AE+G DK+R AE
Sbjct 241 PTKAVSPYPLPLSTNKVGSEAGDAVCGKASLKTAMQYSCNNVFLDVAAEMGEDKMRETAE 300
Query 895 KFGFNKEVTIP----IRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNG 1062
KFGFNK+V + A S +P GTA+ +GQ +TP+QMAMV AA+AN+G
Sbjct 301 KFGFNKDVYAEEFGDMLATKSLYPEKLDKPGTALTGMGQGSLTSTPMQMAMVTAALANDG 360
Query 1063 SLMEPYLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGV 1239
LM+P++V Q G + + + ++SQAVSP TA+K+Q++ME + G+ A+I GV
Sbjct 361 KLMQPHIVDQLRGPDLSTLEKNEPAEMSQAVSPETAKKIQEMMEHTAKEGSAQRAQINGV 420
Query 1240 TVGGKTGTAQHGQD-NSGLPFAWFVSYAKGQDGKQVAVAVVVE--DGSSEAQQISGGSLA 1410
TVGGKTGTAQ G + +P+ WFVSY K DG+ VAVAV ++ D ISGG L
Sbjct 421 TVGGKTGTAQRGVNVRDQVPYGWFVSYGKKDDGRSVAVAVFIDPTDMDISRSDISGGRLG 480
Query 1411 APIAKAMMQAALGK 1452
APIAK +M+A LG+
Sbjct 481 APIAKKVMEAVLGE 494
>WP_003981922.1 MULTISPECIES: penicillin-binding protein 2 [Streptomycetaceae]
KOG83773.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=489
Score = 439 bits (1130), Expect = 2e-150, Method: Compositional matrix adjust.
Identities = 233/490 (48%), Positives = 313/490 (64%), Gaps = 7/490 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+N+P+R +++FC VL+LAL++R WVQ VQA AN+ +N R K + ++YPRGNIIVGG+
Sbjct 1 MNRPLRHIAVFCGVLVLALLVRATWVQFVQADALANDDNNRRVKIEAFSYPRGNIIVGGK 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
+T S G +KYKR + DGPMYAP+TGY+SQ+ + LE + ILSG D RLF +
Sbjct 61 PITGSVETAG-DFKYKRVYKDGPMYAPITGYASQSQGTTFLEGVYKDILSGKDDRLFLKR 119
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLG--NKKGAAVAIDPKTGAILGLVSTPSYD 534
D+LTG+ +GGDV+TTI+ K Q+ ++ L N KGA VA++P TG IL + STPSYD
Sbjct 120 AKDVLTGEKPRGGDVLTTINEKAQRTAYKALTDLNAKGAVVALEPATGKILAMASTPSYD 179
Query 535 PGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSD 714
P FAG + + +T+L D KPM NRA E YPPGSTFK++TAA A E G
Sbjct 180 PSVFAGSSFKEGDKYTSLIKDKAKPMNNRASLEIYPPGSTFKILTAAAALEHGIVPDIDT 239
Query 715 VTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAE 894
P Y LP T+T + N T C NA+++ M SCN + + LG DK+R AE
Sbjct 240 PVQAPVPYTLPQTATKVGNDIATAPCANASLKVGMQWSCNNTYLEAANRLGTDKMRETAE 299
Query 895 KFGFNKEVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLME 1074
KFGFN+++ P+R S +P TA+ +GQ +TPLQMAMV A +ANNG +M+
Sbjct 300 KFGFNQKIFTPVRTATSGYPKKLDKPQTALTGMGQGSLTSTPLQMAMVVAGLANNGKVMQ 359
Query 1075 PYLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGG 1251
PY+V + G + I +LSQAVS +TA+K+Q++ME+ ++GT A I GV VG
Sbjct 360 PYMVEETRGPDLSTIEKFEPKELSQAVSESTAKKVQEMMENTAENGTAKKALIDGVKVGA 419
Query 1252 KTGTAQHGQD-NSGLPFAWFVSYAKGQDGKQVAVAVVVE--DGSSEAQQISGGSLAAPIA 1422
KTGTAQHG D P+AWFVSYAK +G VAVAV V+ D ++I+GG L PIA
Sbjct 420 KTGTAQHGADVRDERPYAWFVSYAKQGNGSPVAVAVFVDPSDMDIPRKEIAGGKLGGPIA 479
Query 1423 KAMMQAALGK 1452
K +M+A LGK
Sbjct 480 KKVMEAVLGK 489
>WP_052836864.1 penicillin-binding protein 2 [Kitasatospora aureofaciens]
Length=495
Score = 436 bits (1122), Expect = 4e-149, Method: Compositional matrix adjust.
Identities = 234/493 (47%), Positives = 319/493 (65%), Gaps = 11/493 (2%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+NKP+R V+IFC +L+L L++R NW+Q V+A A++ N R + +++A PRG+IIVGG+
Sbjct 1 MNKPLRAVAIFCGLLVLGLLIRANWLQYVRAPELASDTKNRRVQIEQFATPRGDIIVGGR 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVT S KYKR++VDGPMYAPVTGY+SQ + LEK D IL+G D RL F+
Sbjct 61 AVTGSKETETTDLKYKRTYVDGPMYAPVTGYASQAQGMTLLEKTYDSILTGKDDRLAFQR 120
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKK---GAAVAIDPKTGAILGLVSTPSY 531
D+++G+ ++ G VVTTIDPK QKA ++GL + K GA VA+DP+TG +L +VS PSY
Sbjct 121 FADVVSGEGRRAGSVVTTIDPKAQKAAWDGLTDLKGARGAVVALDPQTGKVLAMVSAPSY 180
Query 532 DPGTFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPS 711
DP TFAG T + + ALD NKP+ NRALRETYPPGSTFK++TAA A E G
Sbjct 181 DPSTFAGSTFKESDRFVALDKKKNKPLANRALRETYPPGSTFKILTAAAALEHGIVTDVD 240
Query 712 DVTDTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQA 891
TD Y LP ++ + + + C A+++ AM SCN VF ++LG +K+R A
Sbjct 241 ARTDAVSPYPLPQSTNKIGSEAGDAVCNKASMKTAMQHSCNNVFLDAASKLGQEKMRETA 300
Query 892 EKFGFNKEVTI----PIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANN 1059
EKFGFN++V + A S +P GTA+ +GQ +TP+QMA+V A IAN+
Sbjct 301 EKFGFNEDVYSDDFGDLLATKSLYPEDLDKPGTALTGMGQGSLTSTPMQMALVTAGIAND 360
Query 1060 GSLMEPYLVAQ-EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPG 1236
G +M+PY+V + +G + + +S+AVS TAQK+Q++ME + G+ A+I G
Sbjct 361 GKVMQPYVVDEVKGPDLDTLEKTEPAVMSEAVSAETAQKVQEMMEFTAKEGSARRAQIDG 420
Query 1237 VTVGGKTGTAQHGQD-NSGLPFAWFVSYAKGQDGKQVAVAVVVE--DGSSEAQQISGGSL 1407
VTVGGKTGTAQ G D N +P+ WFVSY K DG VAVAV ++ D ISGG L
Sbjct 421 VTVGGKTGTAQRGVDVNDEVPYGWFVSYGKKDDGSSVAVAVFIDPTDMDISRADISGGGL 480
Query 1408 AAPIAKAMMQAAL 1446
APIAK++M+A L
Sbjct 481 GAPIAKSVMKAVL 493
>WP_078656350.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=502
Score = 435 bits (1119), Expect = 1e-148, Method: Compositional matrix adjust.
Identities = 240/483 (50%), Positives = 311/483 (64%), Gaps = 6/483 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR + FCL+L+ AL ++ VQ +++ +N N R ++YA PRG+I+VGG+ VT S
Sbjct 22 RRAATFCLLLVGALCVQATRVQVFESADLDHNTANQRLTIERYARPRGDILVGGRPVTGS 81
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY YKR + DGP YA VTG++SQ Y SQLE EDG+L+GTD RL + D +
Sbjct 82 RPTGG-RYAYKRGYTDGPTYAAVTGFASQAYGNSQLEGTEDGLLTGTDGRLTGWSLWDAI 140
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + GGDV TTIDP Q+A GLG +KGA AI+P TG IL L STPSYDPG FAG
Sbjct 141 TRRQNPGGDVHTTIDPAAQQAAMSGLGGRKGAVAAIEPATGRILALASTPSYDPGDFAGS 200
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D AW L D ++PMLNRALR+TYPPGSTFK+VTAA A G P TD P
Sbjct 201 SGADRTAWNRLRDDTDQPMLNRALRQTYPPGSTFKVVTAAAALSAGVVTDPGAPTDAPFP 260
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+TPL+N S D ++ AM SCN+V G +G G K+ A AE FGFN
Sbjct 261 YVLPGTTTPLVNDSADCDRPGLSLDAAMTASCNSVLGYLGVRTGLSKVVATAEGFGFNDP 320
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ +P+R S+ + P A+ +IGQ DT ATPL MAMVAA IAN+G++M P LV +
Sbjct 321 RLDVPVRPVRSNVDTSMDPAELALSSIGQFDTAATPLVMAMVAAGIANDGTVMRPQLVDK 380
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + +AV+PA A ++++LM VV++GTG NA+I G VGGKTGTAQ
Sbjct 381 LTRSDGTEVELMRPAVYRRAVTPAVAGQVRKLMTDVVENGTGGNARIAGAVVGGKTGTAQ 440
Query 1270 HGQDNSGLPFAWFVSYA--KGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAA 1443
HG DNSG P+AWF+S+A G D VAV V S+A ++GG+LAAPIA+++M+A
Sbjct 441 HGVDNSGTPYAWFISWAGPAGSD-AVPPVAVAVVVADSDATDVTGGALAAPIARSVMRAV 499
Query 1444 LGK 1452
LG+
Sbjct 500 LGR 502
>OEV33654.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=486
Score = 432 bits (1112), Expect = 1e-147, Method: Compositional matrix adjust.
Identities = 239/483 (49%), Positives = 309/483 (64%), Gaps = 6/483 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR + FCL+L+ AL ++ Q +++ +N N R ++YA PRG+I+VGG+ VT S
Sbjct 6 RRAATFCLLLVGALCVQATRAQVFESADLDHNTANQRLTIERYARPRGDILVGGRPVTGS 65
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY YKR + DGP YA VTG++SQ Y SQLE EDG+L+GTD RL + D +
Sbjct 66 RPTGG-RYAYKRGYTDGPTYAAVTGFASQAYGNSQLEGTEDGLLTGTDGRLTGWSLWDAI 124
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + GGDV TTIDP Q+A GLG +KGA AI+P TG IL L STPSYDPG FAG
Sbjct 125 TRRQNPGGDVHTTIDPAAQQAAMSGLGGRKGAVAAIEPATGRILALASTPSYDPGDFAGS 184
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D AW L D ++PMLNRALR+TYPPGSTFK+VTAA A G P TD P
Sbjct 185 SGADRTAWNRLRDDTDQPMLNRALRQTYPPGSTFKVVTAAAALSAGVVTDPGAPTDAPFP 244
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+TPL+N S D ++ AM SCN+V G +G G K+ A AE FGFN
Sbjct 245 YVLPGTTTPLVNDSADCDRPGLSLDAAMTASCNSVLGYLGVRTGLSKVVATAEGFGFNDP 304
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ +P+R S+ + P A+ +IGQ DT ATPL MAMVAA IAN+G++M P LV +
Sbjct 305 RLDVPVRPVRSNVDTSMDPAELALSSIGQFDTAATPLVMAMVAAGIANDGTVMRPQLVDK 364
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + +AV+PA A ++++LM VV+ GTG NA+I G VGGKTGTAQ
Sbjct 365 LTRSDGTEVELMRPAVYRRAVTPAVAGQVRKLMTDVVEDGTGGNARIAGAVVGGKTGTAQ 424
Query 1270 HGQDNSGLPFAWFVSYA--KGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAA 1443
HG DNSG P+AWF+S+A G D VAV V S+A ++GG+LAAPIA+++M+A
Sbjct 425 HGVDNSGTPYAWFISWAGPAGSD-AVPPVAVAVVVADSDATDVTGGALAAPIARSVMRAV 483
Query 1444 LGK 1452
LG+
Sbjct 484 LGR 486
>WP_078938872.1 MULTISPECIES: penicillin-binding protein [Streptomycetaceae]
ARF78986.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=502
Score = 432 bits (1112), Expect = 1e-147, Method: Compositional matrix adjust.
Identities = 239/483 (49%), Positives = 309/483 (64%), Gaps = 6/483 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR + FCL+L+ AL ++ Q +++ +N N R ++YA PRG+I+VGG+ VT S
Sbjct 22 RRAATFCLLLVGALCVQATRAQVFESADLDHNTANQRLTIERYARPRGDILVGGRPVTGS 81
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY YKR + DGP YA VTG++SQ Y SQLE EDG+L+GTD RL + D +
Sbjct 82 RPTGG-RYAYKRGYTDGPTYAAVTGFASQAYGNSQLEGTEDGLLTGTDGRLTGWSLWDAI 140
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + GGDV TTIDP Q+A GLG +KGA AI+P TG IL L STPSYDPG FAG
Sbjct 141 TRRQNPGGDVHTTIDPAAQQAAMSGLGGRKGAVAAIEPATGRILALASTPSYDPGDFAGS 200
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D AW L D ++PMLNRALR+TYPPGSTFK+VTAA A G P TD P
Sbjct 201 SGADRTAWNRLRDDTDQPMLNRALRQTYPPGSTFKVVTAAAALSAGVVTDPGAPTDAPFP 260
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+TPL+N S D ++ AM SCN+V G +G G K+ A AE FGFN
Sbjct 261 YVLPGTTTPLVNDSADCDRPGLSLDAAMTASCNSVLGYLGVRTGLSKVVATAEGFGFNDP 320
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ +P+R S+ + P A+ +IGQ DT ATPL MAMVAA IAN+G++M P LV +
Sbjct 321 RLDVPVRPVRSNVDTSMDPAELALSSIGQFDTAATPLVMAMVAAGIANDGTVMRPQLVDK 380
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + +AV+PA A ++++LM VV+ GTG NA+I G VGGKTGTAQ
Sbjct 381 LTRSDGTEVELMRPAVYRRAVTPAVAGQVRKLMTDVVEDGTGGNARIAGAVVGGKTGTAQ 440
Query 1270 HGQDNSGLPFAWFVSYA--KGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAA 1443
HG DNSG P+AWF+S+A G D VAV V S+A ++GG+LAAPIA+++M+A
Sbjct 441 HGVDNSGTPYAWFISWAGPAGSD-AVPPVAVAVVVADSDATDVTGGALAAPIARSVMRAV 499
Query 1444 LGK 1452
LG+
Sbjct 500 LGR 502
>BAJ30397.1 putative penicillin-binding protein A [Kitasatospora setae KM-6054]
Length=504
Score = 431 bits (1108), Expect = 6e-147, Method: Compositional matrix adjust.
Identities = 243/482 (50%), Positives = 311/482 (65%), Gaps = 4/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FC++LI+AL L+ VQ Q +N N R +YA PRGN++VGGQ VT S
Sbjct 24 RRAGTFCMLLIVALALQATRVQVFQKKELDHNSANQRATIQRYAQPRGNLLVGGQPVTGS 83
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY Y+R++ DGP+YA VTGYSSQTY +QLE ++D +LSGTD RL D +
Sbjct 84 SATGG-RYAYQRTYTDGPLYAAVTGYSSQTYGNTQLEGVDDDLLSGTDPRLAGWAVWDAV 142
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + + GGDVVTTIDP Q+A GLGN+KGA AI+P TG IL L STPSYDPG+FAG
Sbjct 143 TRQQQPGGDVVTTIDPAAQRAALRGLGNQKGAVAAIEPATGRILALASTPSYDPGSFAGT 202
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D+ AW L DP++PMLNRALR+ YPPGSTFK+VTAA A G T P
Sbjct 203 SNADQAAWKKLQDDPDQPMLNRALRQIYPPGSTFKVVTAAAALANGVVTDIDAPTGAPYP 262
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y++PGT+TPL N + D ++ AM LSCN+V G +G + G D++ A A FGFN
Sbjct 263 YVMPGTTTPLNNDTTACDQAGLSLDEAMTLSCNSVMGYLGVQTGLDRMTAMAGNFGFNDA 322
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ P+RA S+F + A+ +IGQ+DT ATPL MAMVAA AN+G +M P LV +
Sbjct 323 RLDTPVRAARSNFDTDMNKSQLALSSIGQYDTAATPLVMAMVAAGAANDGQVMYPQLVDR 382
Query 1093 EGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
A G+ + QA TAQ+L+QLM +VV++GTG NA+IPG VGGKTGTAQ
Sbjct 383 LTKADGSQVQLMHPSLYHQAFGAGTAQQLRQLMVNVVENGTGRNARIPGAEVGGKTGTAQ 442
Query 1270 HGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG DNSG P+AWF+++A+ VAV V S+A ++GG LAAPIAK++MQA L
Sbjct 443 HGVDNSGTPYAWFIAWARPSGSAAVPPVAVAVVIADSQATDVTGGGLAAPIAKSVMQAVL 502
Query 1447 GK 1452
K
Sbjct 503 DK 504
>WP_057228032.1 MULTISPECIES: penicillin-binding protein [Kitasatospora]
KQV24303.1 penicillin-binding protein [Kitasatospora sp. Root107]
KRB67648.1 penicillin-binding protein [Kitasatospora sp. Root187]
Length=468
Score = 420 bits (1080), Expect = 4e-143, Method: Compositional matrix adjust.
Identities = 229/466 (49%), Positives = 300/466 (64%), Gaps = 4/466 (1%)
Frame = +1
Query 58 MLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLRYKYKRSW 237
M + +VQ +A + +NP N R +YA PRGNI+VG VT S G RY YKR++
Sbjct 1 MGQATYVQVFRADSLDDNPANQRETIQRYAQPRGNILVGADPVTGSAPTGG-RYAYKRTY 59
Query 238 VDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQGGDVVTTI 417
DGPM+A VTGYSSQTY +Q+E +ED +LSGTDSRL D +T K GGDV TT+
Sbjct 60 TDGPMWAAVTGYSSQTYGNTQIEGVEDDLLSGTDSRLASWALWDEITRKQNPGGDVYTTL 119
Query 418 DPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKAWTALDSD 597
+ Q+A +GLGN+ GA AI+P TG IL L STPSYDPG+FAG + D+ AW L +D
Sbjct 120 NNAAQQAAMKGLGNQTGAVAAIEPATGRILALASTPSYDPGSFAGSSTADQDAWNKLQAD 179
Query 598 PNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTSTPLINAS 777
N+PMLNRALR+ YPPGSTFK+VTAA A G T P +++PGT+T L+N +
Sbjct 180 KNQPMLNRALRQIYPPGSTFKVVTAAAALANGVVTDIDAPTSAPYPFVMPGTTTALVNDT 239
Query 778 PTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK-EVTIPIRADASHFP 954
D ++ AM SCN+V G +G ++G DK+ A A+ FGFN ++ P+RA S+F
Sbjct 240 SACDTPGQSLDQAMVNSCNSVMGYLGTQVGLDKMVAMAQNFGFNDAKLDTPVRAARSNFD 299
Query 955 SGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ-EGSAGNVISTHTE 1131
+ A+ +IGQ+DT ATPL MAMVAA +ANNG++M P LV + S G+ +
Sbjct 300 TAMNASQVALSSIGQYDTAATPLVMAMVAAGVANNGTVMYPQLVDKLTKSDGSSVQVMQP 359
Query 1132 HQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSGLPFAWFV 1311
QA+SP A ++QQLM VV+ GTG +A+I G TVGGKTGTAQ G DNSG+P+AWF+
Sbjct 360 AGYRQAMSPTVAGQVQQLMTDVVETGTGRSARISGATVGGKTGTAQQGIDNSGIPYAWFI 419
Query 1312 SYAKGQDGKQVAVAVVVED-GSSEAQQISGGSLAAPIAKAMMQAAL 1446
S+AK QV V S A ++GG LAAPIA+++MQA L
Sbjct 420 SWAKPSGSNQVPPVAVAVVIADSNATDVTGGGLAAPIARSVMQAVL 465
>WP_051055920.1 penicillin-binding protein [Kitasatospora setae]
Length=474
Score = 420 bits (1080), Expect = 5e-143, Method: Compositional matrix adjust.
Identities = 239/475 (50%), Positives = 307/475 (65%), Gaps = 4/475 (1%)
Frame = +1
Query 37 LVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLR 216
++LI+AL L+ VQ Q +N N R +YA PRGN++VGGQ VT S G R
Sbjct 1 MLLIVALALQATRVQVFQKKELDHNSANQRATIQRYAQPRGNLLVGGQPVTGSSATGG-R 59
Query 217 YKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQG 396
Y Y+R++ DGP+YA VTGYSSQTY +QLE ++D +LSGTD RL D +T + + G
Sbjct 60 YAYQRTYTDGPLYAAVTGYSSQTYGNTQLEGVDDDLLSGTDPRLAGWAVWDAVTRQQQPG 119
Query 397 GDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKA 576
GDVVTTIDP Q+A GLGN+KGA AI+P TG IL L STPSYDPG+FAG + D+ A
Sbjct 120 GDVVTTIDPAAQRAALRGLGNQKGAVAAIEPATGRILALASTPSYDPGSFAGTSNADQAA 179
Query 577 WTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTS 756
W L DP++PMLNRALR+ YPPGSTFK+VTAA A G T P Y++PGT+
Sbjct 180 WKKLQDDPDQPMLNRALRQIYPPGSTFKVVTAAAALANGVVTDIDAPTGAPYPYVMPGTT 239
Query 757 TPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK-EVTIPIR 933
TPL N + D ++ AM LSCN+V G +G + G D++ A A FGFN + P+R
Sbjct 240 TPLNNDTTACDQAGLSLDEAMTLSCNSVMGYLGVQTGLDRMTAMAGNFGFNDARLDTPVR 299
Query 934 ADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSA-GN 1110
A S+F + A+ +IGQ+DT ATPL MAMVAA AN+G +M P LV + A G+
Sbjct 300 AARSNFDTDMNKSQLALSSIGQYDTAATPLVMAMVAAGAANDGQVMYPQLVDRLTKADGS 359
Query 1111 VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSG 1290
+ QA TAQ+L+QLM +VV++GTG NA+IPG VGGKTGTAQHG DNSG
Sbjct 360 QVQLMHPSLYHQAFGAGTAQQLRQLMVNVVENGTGRNARIPGAEVGGKTGTAQHGVDNSG 419
Query 1291 LPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAALGK 1452
P+AWF+++A+ VAV V S+A ++GG LAAPIAK++MQA L K
Sbjct 420 TPYAWFIAWARPSGSAAVPPVAVAVVIADSQATDVTGGGLAAPIAKSVMQAVLDK 474
>WP_033819927.1 penicillin-binding protein [Kitasatospora sp. MBT63]
Length=487
Score = 419 bits (1078), Expect = 1e-142, Method: Compositional matrix adjust.
Identities = 231/480 (48%), Positives = 307/480 (64%), Gaps = 4/480 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FC++LI+AL+ + VQ + +NP N R +Y PRGNI+V GQ VT S
Sbjct 6 RRAGTFCVLLIVALIAQATRVQVFRKDELDHNPANQRLTVQRYDQPRGNILVDGQPVTGS 65
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G R+ Y+R++ DGP YA VTG+SSQTY +QLE + D +LSGTDSRL D +
Sbjct 66 TPTGG-RFDYERTYTDGPRYAAVTGFSSQTYGNTQLEGVYDDLLSGTDSRLAGWALWDAV 124
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ + GGDVVTT+D Q A +GLG ++GA VAI+P TG IL L STPSYDP +FAG
Sbjct 125 SRRQNPGGDVVTTLDRAAQDAAVKGLGRQQGAVVAIEPATGRILALASTPSYDPRSFAGS 184
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
D +AW L +DP++PMLNRALR+ YPPGSTFK+VTAA A + G P+ T P
Sbjct 185 GAADRRAWDRLQADPDQPMLNRALRQIYPPGSTFKVVTAAAALQDGVVSDPAAPTGAPSP 244
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L+N + D + + AM LSCN+V G +G ++G + + + AE FGFN
Sbjct 245 YVLPGTTTTLVNDTAACDRPDLPLDTAMVLSCNSVLGYLGVQVGQEGMVSMAENFGFNDA 304
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ +P+RA S+F + +P A+ +IGQ DT ATPL MAMVAA +AN G +M P LV +
Sbjct 305 SLDVPVRAARSNFDTEMEPSQLALSSIGQFDTAATPLVMAMVAAGVANGGRVMYPQLVDR 364
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + +A+ A A ++Q LM VV+ GTG A+IPG TVGGKTGTAQ
Sbjct 365 LTKSDGTAVQVMRPELYKEAMGAAVAGQVQGLMTDVVEQGTGRAARIPGATVGGKTGTAQ 424
Query 1270 HGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG NSG+P+AWF+S+A+ VAV V S+A ++GG LAAPIA+++MQA L
Sbjct 425 HGVGNSGVPYAWFISWARPAGSADPPPVAVAVVVADSDATDVTGGRLAAPIARSVMQAVL 484
>WP_051817654.1 penicillin-binding protein [Kitasatospora sp. NRRL B-11411]
Length=474
Score = 413 bits (1062), Expect = 2e-140, Method: Compositional matrix adjust.
Identities = 235/475 (49%), Positives = 304/475 (64%), Gaps = 4/475 (1%)
Frame = +1
Query 37 LVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLR 216
++LI+AL ++ VQ Q +N N R +YA PRGN++V GQ VT S G R
Sbjct 1 MLLIVALAVQATRVQVFQKHDLDHNSANQRATIQRYAQPRGNLLVAGQPVTGSAPTGG-R 59
Query 217 YKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQG 396
Y YKR++ DG +YAPVTGYSSQTY +QLE + D +LSGTD RL D +T + + G
Sbjct 60 YAYKRTYTDGALYAPVTGYSSQTYGNTQLEGVYDDLLSGTDPRLAGWAVWDAVTRQQQPG 119
Query 397 GDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKA 576
GDVVTTIDP Q+A +GLGN+KGA AI+P TG IL L STP YDPGTFAG + D+ A
Sbjct 120 GDVVTTIDPAAQRAAMQGLGNQKGAVAAIEPATGRILALASTPGYDPGTFAGTSAADQNA 179
Query 577 WTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTS 756
W L DP++PMLNRALR+ YPPGSTFK+VTAA A G TD P Y++PGT+
Sbjct 180 WKKLQDDPDQPMLNRALRQIYPPGSTFKVVTAAAALANGVVTDIEAPTDAPYPYVMPGTT 239
Query 757 TPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK-EVTIPIR 933
TPL N + D ++ AM LSCN+V G +G + G D++ A A+ FGFN + P+R
Sbjct 240 TPLNNDTSACDRAGLSLDEAMTLSCNSVMGYLGVQTGLDRMTAMAKNFGFNDARLDTPVR 299
Query 934 ADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSA-GN 1110
A S+F + A+ +IGQ+DT ATPL MAMVAA A +G +M P LV + A G+
Sbjct 300 AARSNFDTEMNKSQLALSSIGQYDTAATPLAMAMVAAGAAADGQVMYPQLVDKLTKADGS 359
Query 1111 VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSG 1290
+ QA A AQ+L+QLM VV++GTG NA+IPG VGGKTGTAQHG DNSG
Sbjct 360 QVQLMHPSLYRQAFGAAVAQQLRQLMVDVVENGTGRNARIPGAEVGGKTGTAQHGVDNSG 419
Query 1291 LPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAALGK 1452
P+AWF+++A+ VAV V S+A ++GG LAAPIA+++MQ L +
Sbjct 420 TPYAWFIAWARPAGSTAVPPVAVAVVVADSQADDVTGGGLAAPIARSVMQTVLNR 474
>WP_051776935.1 penicillin-binding protein [Kitasatospora phosalacinea]
Length=474
Score = 412 bits (1060), Expect = 5e-140, Method: Compositional matrix adjust.
Identities = 231/478 (48%), Positives = 310/478 (65%), Gaps = 10/478 (2%)
Frame = +1
Query 37 LVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLR 216
++LI+AL L+ VQ Q +N N R +Y+ PRGN++V GQ VT S G R
Sbjct 1 MLLIVALALQATRVQVFQKDELDHNAANQRATIQRYSQPRGNLLVAGQPVTGSAETGG-R 59
Query 217 YKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQG 396
Y Y+R++ DGP+YA VTGYSSQTY +QLE + D +LSGTD +L D +T + + G
Sbjct 60 YAYQRTYTDGPLYAAVTGYSSQTYGNTQLEGVYDDVLSGTDPKLAGWAVWDAVTRQQQPG 119
Query 397 GDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKA 576
GDVVTTIDP Q+A +GLG++KGA AI+P TG IL LVSTPSYDPG+FAG + D+ A
Sbjct 120 GDVVTTIDPAAQRAAVQGLGSQKGAVAAIEPATGRILALVSTPSYDPGSFAGTSAADQNA 179
Query 577 WTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTS 756
W L DP +PMLNRALR+ YPPGSTFK+VTAA A +G TD P Y++PGT+
Sbjct 180 WKKLQDDPEQPMLNRALRQIYPPGSTFKVVTAAAALASGVVTDVDAPTDAPYPYVMPGTT 239
Query 757 TPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK-EVTIPIR 933
TPL N + + ++ AM LSCN+V G +G + G D++ A A+ FGFN + +P+R
Sbjct 240 TPLDNDTSACNRAGLSLDEAMTLSCNSVMGYLGVQTGLDRMTAMAQDFGFNDARLDVPVR 299
Query 934 ADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ----EGS 1101
A S+F + A+ +IGQ+DT ATPL MAMVAA AN+G +M P LV + +G+
Sbjct 300 AARSNFDTDMNQSQLALSSIGQYDTAATPLVMAMVAAGAANDGQVMSPQLVDKLTKADGT 359
Query 1102 AGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQD 1281
++ H QA A++L+QLM VV++GTG NA+IPG VGGKTGTAQHG D
Sbjct 360 QVQLMHPALHH---QAFGAGVARQLRQLMVDVVENGTGRNARIPGAEVGGKTGTAQHGVD 416
Query 1282 NSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAALGK 1452
NSG P+AWF+++A+ VAV V S+A ++GG LAAP+A+++MQA L +
Sbjct 417 NSGTPYAWFIAWARPAGSTAVPPVAVAVVVADSQATDVTGGGLAAPVARSVMQAVLNR 474
>WP_052840719.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=485
Score = 412 bits (1058), Expect = 1e-139, Method: Compositional matrix adjust.
Identities = 232/488 (48%), Positives = 317/488 (65%), Gaps = 9/488 (2%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+ + IR ++FC +L++AL++ VQ VQ + +NP N R +Y PRG+I+VGG+
Sbjct 1 MTRNIRHAALFCALLLVALVVNAARVQIVQKPEYDDNPANRRPDIARYQQPRGDILVGGE 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
AVT S G +++R++ DGPMYAP+TG++SQ Y + LE EDG+LSG D L
Sbjct 61 AVTGSRD-TGEHLRFERTYADGPMYAPITGFASQAYGTTLLEHAEDGLLSGADPMLAPLP 119
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
+ +TG GGDVVTTID Q+A FEGLG +KGA A++P TG +L LVSTPSYDP
Sbjct 120 LWNDVTGAHNPGGDVVTTIDGAAQRAAFEGLGGRKGAVAAVEPATGRVLALVSTPSYDPQ 179
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
+G + D + W L+ DP+KPMLNRA+ TYPPGSTFK+VTAA A + G + T
Sbjct 180 LLSGNSADATRTWHRLNDDPDKPMLNRAVSRTYPPGSTFKVVTAAAALDAGVVRDLDAPT 239
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
+PD Y LPGT T L N + + C NA+++ A + SCNTVF K+G ++G + A A+ F
Sbjct 240 RSPDPYTLPGTRTKLTNEA--DGCRNASLREAFEWSCNTVFAKLGVDVGVRGMTAMADAF 297
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + + IP S F + + +IGQ++TRATPLQMAMVAAA+A G + P
Sbjct 298 GFNDDGLRIPFPVARSTFDTSVDRAQLGLSSIGQYNTRATPLQMAMVAAAVAGGGQVRSP 357
Query 1078 YLVAQEGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
YLV + A G++++ + + P+TA L++LM VV GTG A IPG TVGGK
Sbjct 358 YLVERTLRAGGSLVAAAGSRPSREVMRPSTAALLKELMTGVVDKGTGARAAIPGATVGGK 417
Query 1255 TGTAQHGQDNSGLPFAWFVSYAKGQDGK---QVAVAVVVEDGSSEAQQISGGSLAAPIAK 1425
TGTAQHG NSG P+AWF+++A+G DG +VAVAVVVE +++ +SGG LAAPIA+
Sbjct 418 TGTAQHGVGNSGKPYAWFIAWARG-DGDVEPKVAVAVVVEGSAADRGGVSGGGLAAPIAR 476
Query 1426 AMMQAALG 1449
+M+A LG
Sbjct 477 GVMEAVLG 484
>WP_051732513.1 penicillin-binding protein [Kitasatospora phosalacinea]
Length=474
Score = 410 bits (1053), Expect = 5e-139, Method: Compositional matrix adjust.
Identities = 232/475 (49%), Positives = 307/475 (65%), Gaps = 4/475 (1%)
Frame = +1
Query 37 LVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKSDFVNGLR 216
++LI+AL L+ VQ Q +N N R +Y+ PRGN++V GQ VT S G R
Sbjct 1 MLLIVALALQATRVQVFQKKELDHNSANQRATIQRYSQPRGNLLVAGQPVTGSAETGG-R 59
Query 217 YKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDMLTGKPKQG 396
Y YKR++ DG +YA VTGYSSQTY +QLE + D +LSGTD RL D +T + + G
Sbjct 60 YAYKRTYTDGALYAAVTGYSSQTYGNTQLEGVYDDLLSGTDPRLAGWAVWDAVTRQQQPG 119
Query 397 GDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGGTKDDEKA 576
GDVVTTIDP Q+A +GLGN+KGA AI+P TG IL L STP YDPG+FAG + D+ A
Sbjct 120 GDVVTTIDPAAQRAATQGLGNQKGAVAAIEPATGRILALASTPGYDPGSFAGTSTADQAA 179
Query 577 WTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQYILPGTS 756
W L DP++PMLNRALR+ YPPGSTFK+VTAA A G T P Y++PGT+
Sbjct 180 WEKLQDDPDQPMLNRALRQIYPPGSTFKVVTAAAALANGVVTDIDAPTGAPYPYVMPGTT 239
Query 757 TPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK-EVTIPIR 933
TPL N + D ++ A+ LSCN+V G +G ++G D++ A A FGFN ++ +P+R
Sbjct 240 TPLNNDTGACDRAGLSLDEAVTLSCNSVMGYLGVQVGLDRMTAMARNFGFNDAKLDVPVR 299
Query 934 ADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQEGSA-GN 1110
A ++F + A+ +IGQ+DT ATPL MAMVAA AN+G +M P LV + A G+
Sbjct 300 AARANFDTDMNESQLALSSIGQYDTAATPLVMAMVAAGAANDGQVMYPQLVDKLTKADGS 359
Query 1111 VISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQHGQDNSG 1290
+ QA + A++LQQLM VV++GTG NA+IPG VGGKTGTAQHG DN+G
Sbjct 360 QVQLMHPTLYRQAFGSSVARQLQQLMVGVVENGTGRNARIPGAEVGGKTGTAQHGVDNTG 419
Query 1291 LPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAALGK 1452
P+AWF+++A+ VAV V S+A ++GG LAAPIAK++MQA LG+
Sbjct 420 TPYAWFIAWARPAGSAAVPPVAVAVVVADSQADDVTGGGLAAPIAKSVMQAVLGR 474
>WP_100892658.1 penicillin-binding protein [Kitasatospora sp. OK780]
Length=487
Score = 408 bits (1048), Expect = 4e-138, Method: Compositional matrix adjust.
Identities = 236/482 (49%), Positives = 303/482 (63%), Gaps = 8/482 (2%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FCL+LI+AL + +Q A + +N N R +Y PRGNI+VGG+ VT S
Sbjct 6 RRAGTFCLLLIVALAAQATKIQVFDADSLDHNAANQRLAVQRYDQPRGNILVGGRPVTGS 65
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY +KR++ DGP +A VTGYSSQTY SQLE + D +LSGTD RL D L
Sbjct 66 TATGG-RYDWKRTYTDGPRWAAVTGYSSQTYGNSQLEGIYDDLLSGTDDRLSGWAVWDAL 124
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ + GGDV TT+D Q A +GLG++KGA VAI+P TG IL L STPSYDPG+FAG
Sbjct 125 SRRQNPGGDVDTTLDTDAQAAALQGLGSQKGAVVAIEPATGRILALASTPSYDPGSFAGT 184
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D AW L +D ++PMLNRALR+ YPPGSTFK+VTAA A G P P
Sbjct 185 SAADRAAWDRLHADADQPMLNRALRQIYPPGSTFKVVTAAAALAGGVVTDPDAPVGAPSP 244
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L+N + D T+ AM SCN+V G +G + G + L A AE FGFN
Sbjct 245 YVLPGTTTELVNDTSACDGTGITLTEAMVNSCNSVMGYLGVKTGLNGLTAMAENFGFNDA 304
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ P+RA S+ + A+ AIGQ+DT ATPL MAMVAA +ANNG++M P LV +
Sbjct 305 RLDTPVRAARSNIDTTMNASQVALSAIGQYDTAATPLVMAMVAAGVANNGTVMRPQLVDR 364
Query 1093 --EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTA 1266
G + H E +A++ + A ++QQLM VV++GTG A+I G TVGGKTGTA
Sbjct 365 LTRGDGTPIQLMHPE-VYRRAMNGSVAGQVQQLMVDVVENGTGRRARISGATVGGKTGTA 423
Query 1267 QHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGS--SEAQQISGGSLAAPIAKAMMQA 1440
QHG DNSG P+AWF+S+A+ DG V V SEA ++GG +AAPIA+A+MQA
Sbjct 424 QHGVDNSGTPYAWFISWAR-PDGSNAVPPVAVAVVVADSEATDVTGGGIAAPIARAVMQA 482
Query 1441 AL 1446
L
Sbjct 483 VL 484
>PKB48172.1 peptidoglycan glycosyltransferase [Kitasatospora sp. OK780]
Length=502
Score = 408 bits (1048), Expect = 9e-138, Method: Compositional matrix adjust.
Identities = 236/482 (49%), Positives = 303/482 (63%), Gaps = 8/482 (2%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FCL+LI+AL + +Q A + +N N R +Y PRGNI+VGG+ VT S
Sbjct 21 RRAGTFCLLLIVALAAQATKIQVFDADSLDHNAANQRLAVQRYDQPRGNILVGGRPVTGS 80
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G RY +KR++ DGP +A VTGYSSQTY SQLE + D +LSGTD RL D L
Sbjct 81 TATGG-RYDWKRTYTDGPRWAAVTGYSSQTYGNSQLEGIYDDLLSGTDDRLSGWAVWDAL 139
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ + GGDV TT+D Q A +GLG++KGA VAI+P TG IL L STPSYDPG+FAG
Sbjct 140 SRRQNPGGDVDTTLDTDAQAAALQGLGSQKGAVVAIEPATGRILALASTPSYDPGSFAGT 199
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
+ D AW L +D ++PMLNRALR+ YPPGSTFK+VTAA A G P P
Sbjct 200 SAADRAAWDRLHADADQPMLNRALRQIYPPGSTFKVVTAAAALAGGVVTDPDAPVGAPSP 259
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L+N + D T+ AM SCN+V G +G + G + L A AE FGFN
Sbjct 260 YVLPGTTTELVNDTSACDGTGITLTEAMVNSCNSVMGYLGVKTGLNGLTAMAENFGFNDA 319
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ P+RA S+ + A+ AIGQ+DT ATPL MAMVAA +ANNG++M P LV +
Sbjct 320 RLDTPVRAARSNIDTTMNASQVALSAIGQYDTAATPLVMAMVAAGVANNGTVMRPQLVDR 379
Query 1093 --EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTA 1266
G + H E +A++ + A ++QQLM VV++GTG A+I G TVGGKTGTA
Sbjct 380 LTRGDGTPIQLMHPE-VYRRAMNGSVAGQVQQLMVDVVENGTGRRARISGATVGGKTGTA 438
Query 1267 QHGQDNSGLPFAWFVSYAKGQDGKQVAVAVVVEDGS--SEAQQISGGSLAAPIAKAMMQA 1440
QHG DNSG P+AWF+S+A+ DG V V SEA ++GG +AAPIA+A+MQA
Sbjct 439 QHGVDNSGTPYAWFISWAR-PDGSNAVPPVAVAVVVADSEATDVTGGGIAAPIARAVMQA 497
Query 1441 AL 1446
L
Sbjct 498 VL 499
>WP_082346127.1 penicillin-binding protein [Kitasatospora sp. MY 5-36]
Length=502
Score = 406 bits (1043), Expect = 5e-137, Method: Compositional matrix adjust.
Identities = 227/482 (47%), Positives = 306/482 (63%), Gaps = 6/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FCL+L+ AL ++ VQ QA N N R ++YA PRG+I+VG VT S
Sbjct 22 RRAGTFCLLLVAALCVQATRVQVFQAQDLDRNSANQRLTVERYAQPRGDILVGPDPVTGS 81
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
+ G RY YKR + DGP YA VTG++SQ Y +QLE ED +L+GTD RL + D +
Sbjct 82 EPTGG-RYAYKRGYTDGPTYAAVTGFASQAYGTTQLEGTEDELLTGTDGRLAGWSVWDAV 140
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
+ + GGDV TT++ Q+A GLG++KGA AI+P TG IL L STPSYDPG+FAG
Sbjct 141 SRRQNPGGDVHTTLNRAAQQAAMRGLGDQKGAVAAIEPATGRILALASTPSYDPGSFAGY 200
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
DD++AW L +D ++PMLNRALR+TYPPGSTFK+VTAA A G P+ T P
Sbjct 201 GADDQEAWNRLQADADQPMLNRALRQTYPPGSTFKVVTAAAALAAGVVTDPAAPTGAPFP 260
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+TPL+N +P D + ++ AM SCN+V G +G G D++ A GFN
Sbjct 261 YVLPGTTTPLVNDTPACDRADLSLAAAMTASCNSVLGYLGVRTGLDRMAEMAGNLGFNDA 320
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
+ +P+RA S+F + A+ +IGQ DT ATPL MAMVAA +AN+G++M P LV +
Sbjct 321 RLDVPVRAARSNFDTRMDESQLALSSIGQFDTAATPLTMAMVAAGVANDGTVMRPQLVDR 380
Query 1093 -EGSAGNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
S G + +A++PA A +++ LM VV++GTG NA+I G VGGKTGTAQ
Sbjct 381 LTRSDGTTVQLMKPAVYRRAMTPAVAGQVRGLMTGVVENGTGGNARIAGAVVGGKTGTAQ 440
Query 1270 HGQDNSGLPFAWFVSYA--KGQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAA 1443
HG NSG P+AWF+++A G D VV + S A ++GG+LAAPIA+++M+A
Sbjct 441 HGVGNSGTPYAWFIAWAAPAGSDAVPPVAVAVVVE-DSGATDVTGGALAAPIARSVMRAV 499
Query 1444 LG 1449
LG
Sbjct 500 LG 501
>WP_003983996.1 MULTISPECIES: penicillin-binding protein [Streptomycetaceae]
KOG67928.1 penicillin-binding protein [Kitasatospora aureofaciens]
Length=484
Score = 395 bits (1015), Expect = 4e-133, Method: Compositional matrix adjust.
Identities = 249/486 (51%), Positives = 319/486 (66%), Gaps = 7/486 (1%)
Frame = +1
Query 1 VNKPIRRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQ 180
+N+ IRR S+F LVL++AL+L VQ +A + NP N RT +Y+ PRGNI+VGG+
Sbjct 1 MNRYIRRASVFGLVLLVALLLNAARVQVFKADAYDANPANRRTALTRYSQPRGNILVGGR 60
Query 181 AVTKSDFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRN 360
VT S G R +Y+R++ +GP+YA VTGY+SQ Y S LE ED +LSGTD RL
Sbjct 61 PVTGSRDSGG-RLRYERTYTEGPLYAAVTGYASQIYGTSLLEHAEDRVLSGTDPRLAPFP 119
Query 361 TLDMLTGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPG 540
+T + + GG+V TTID Q+A FEGLG+KKGA A++P+TG IL LVSTPSYDP
Sbjct 120 LWTDITREQQPGGEVRTTIDAAAQRAAFEGLGSKKGAVAAVEPRTGKILALVSTPSYDPN 179
Query 541 TFAGGTKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVT 720
AG + AW L+ +PMLNRAL++TYPPGSTFK+VTAA A + K T
Sbjct 180 ELAGTGRPVTTAWDRLNHGGEQPMLNRALKQTYPPGSTFKVVTAAAALDHRKVTDLDAKT 239
Query 721 DTPDQYILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKF 900
P Y LPGTST L N + C NA+++HA ++SCNTVF +G ++G D + AE F
Sbjct 240 GIPSPYTLPGTSTKLTNEA--GGCANASLRHAFEMSCNTVFAGLGVDVGLDGMAGTAENF 297
Query 901 GFNKE-VTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEP 1077
GFN + IP A S+F + A+ AIGQ+DT ATPLQMAMVAAA+AN+G + P
Sbjct 298 GFNDAGLKIPSTATRSNFDTDMNDAQLALSAIGQYDTTATPLQMAMVAAAVANDGGVRAP 357
Query 1078 YLVAQEGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGK 1254
YLV + A G +S + L +A+ TA++LQ+LM VV GTG NA I G TVGGK
Sbjct 358 YLVDKVTDADGETVSATRQRTLHRAMERDTARRLQELMTGVVTRGTGGNAAIDGATVGGK 417
Query 1255 TGTAQHGQDNSGLPFAWFVSYAK--GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKA 1428
TGTAQHG N G P+AWF+S+AK G D VAVAVVVED +++ ISGG AAPIA+A
Sbjct 418 TGTAQHGVHNEGTPYAWFISWAKRDGADDPAVAVAVVVEDAAADRADISGGGSAAPIARA 477
Query 1429 MMQAAL 1446
+MQAAL
Sbjct 478 VMQAAL 483
>WP_083646743.1 penicillin-binding protein [Kitasatospora sp. CB01950]
Length=501
Score = 395 bits (1015), Expect = 6e-133, Method: Compositional matrix adjust.
Identities = 232/484 (48%), Positives = 308/484 (64%), Gaps = 11/484 (2%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR + FC++LI+ L ++ VQ Q N N R +YA PRGN+I G Q VT S
Sbjct 24 RRAATFCMLLIIVLAVQGTRVQVFQKKELDQNSANQRATVQRYAQPRGNLIAG-QPVTGS 82
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G R Y+R++ DGP+YA VTGYSSQ Y +QLE + D +L+GTD RL D +
Sbjct 83 -VATGGRLAYQRTYTDGPLYAAVTGYSSQIYGNTQLEGVYDALLTGTDPRLAGWAVWDAI 141
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + GGDVVTTIDP VQ+A +GLGN+KGA AI+P TG IL L STPSYDPG+ AG
Sbjct 142 TRHQQPGGDVVTTIDPAVQRAAMQGLGNQKGAVAAIEPATGRILALASTPSYDPGSIAGT 201
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
T D+ AW L +DP++PMLNRALR+ YPPGSTFK+VTAA A G T P
Sbjct 202 T--DQAAWEKLQADPDQPMLNRALRQIYPPGSTFKVVTAAAALANGTVTDIQAPTGAPYP 259
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y+LPGT+T L N + + ++ AM +SCN+V G +G + G D++ A A+ FGF+
Sbjct 260 YVLPGTTTTLNNDTGACNQEGLSLDDAMTMSCNSVMGWLGVQTGLDRMVAMAKNFGFDDA 319
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
++ IP+RA S+F + A+ +IGQ++T ATPL MAMVAA A+NG +M P LV +
Sbjct 320 KLDIPVRAARSNFDTDMNASQLALSSIGQYNTAATPLVMAMVAAGAADNGQVMYPQLVDR 379
Query 1093 EGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
A G + QA P A ++++LM VV +GTG NA+IPG VGGKTGTA+
Sbjct 380 LTKADGTQVQLMRPTLYRQAFGPGVAAQVRRLMVDVVDNGTGANAQIPGAEVGGKTGTAE 439
Query 1270 HGQDNSGLPFAWFVSYAK---GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQA 1440
HG DN+G P+AWF+++A+ D VAVAVV+ D S+A ++GG LAAPIAK++MQ
Sbjct 440 HGVDNTGTPYAWFIAWARPAGSTDVPPVAVAVVIAD--SQASDVTGGGLAAPIAKSVMQT 497
Query 1441 ALGK 1452
L +
Sbjct 498 VLNR 501
>KIQ64169.1 penicillin-binding protein [Kitasatospora griseola]
Length=485
Score = 389 bits (999), Expect = 1e-130, Method: Compositional matrix adjust.
Identities = 229/482 (48%), Positives = 303/482 (63%), Gaps = 5/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR + FC++LI+ L ++ VQ Q +N N R +YA PRGN+I GQ VT S
Sbjct 6 RRAATFCMLLIVLLAVQATRVQVFQKQELDHNSANQRATIQRYAQPRGNLI-AGQPVTGS 64
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G R Y+R++ DGP+YA VTGYSSQ Y +QLE + D +L+GTD RL D +
Sbjct 65 -VATGGRLAYRRTYTDGPLYAAVTGYSSQIYGNTQLEGVYDDLLTGTDPRLTGWAVWDAI 123
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + GGDVVTTIDP VQ+A +GLG ++GA AI+P TG IL L STPSYDPGT AG
Sbjct 124 TRNQRPGGDVVTTIDPAVQRAAMQGLGGQQGAVAAIEPATGRILALASTPSYDPGTIAGA 183
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
D+ AW L +DP++PMLNRALR+ YPPGSTFK+VTAA A G T P
Sbjct 184 GAADQAAWEKLQADPDQPMLNRALRQIYPPGSTFKVVTAAAALANGTVTDIQAPTGAPFP 243
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y++PGT+T L N + D ++ AM LSCN+V G +G + G D++ A A+ FGF+
Sbjct 244 YVMPGTTTTLNNDTSACDREGLSLDEAMTLSCNSVLGWLGVQTGLDRMVAMAKNFGFDDA 303
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
++ +P+RA S+F + A+ +IGQ++T ATPL MAMVAA +A +G +M P LV +
Sbjct 304 KLDVPVRAARSNFDTRMNSSQLALSSIGQYNTAATPLVMAMVAAGVAADGQVMHPQLVDR 363
Query 1093 EGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
A G + QA P A +L++LM +VV +GTG NA+IPG VGGKTGTA+
Sbjct 364 LTKADGTQVQLMRPALYRQAFGPGVAAELRRLMVNVVNNGTGGNAQIPGAEVGGKTGTAE 423
Query 1270 HGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG DNSG P+AWF+++A+ VAV V S A ++GG LAAPIAKA+MQA L
Sbjct 424 HGVDNSGTPYAWFIAWARPAGSTATPPVAVAVVVADSTATDVTGGRLAAPIAKAVMQAVL 483
Query 1447 GK 1452
+
Sbjct 484 DR 485
>KDN83954.1 penicillin-binding protein [Kitasatospora cheerisanensis KCTC
2395]
Length=503
Score = 387 bits (995), Expect = 8e-130, Method: Compositional matrix adjust.
Identities = 231/482 (48%), Positives = 305/482 (63%), Gaps = 5/482 (1%)
Frame = +1
Query 16 RRVSIFCLVLILALMLRVNWVQGVQASTWANNPHNDRTKYDKYAYPRGNIIVGGQAVTKS 195
RR FC++LI+AL ++ VQ Q +N N R +YA PRGN+I G Q +T S
Sbjct 24 RRAGTFCMLLIVALAVQATRVQVFQKQELDHNSANQRAVIQRYAQPRGNLIAG-QPLTGS 82
Query 196 DFVNGLRYKYKRSWVDGPMYAPVTGYSSQTYDASQLEKLEDGILSGTDSRLFFRNTLDML 375
G R ++R++ DGP+YA VTGYSSQ Y +QLE + D +L+GTD RL D +
Sbjct 83 KATGG-RLAHQRTYTDGPLYAAVTGYSSQLYGNTQLEGVYDDLLTGTDPRLAGWAVWDAI 141
Query 376 TGKPKQGGDVVTTIDPKVQKAGFEGLGNKKGAAVAIDPKTGAILGLVSTPSYDPGTFAGG 555
T + + GGDVVTTIDP VQ+A +GLG++KGA AI+P TG IL L STPSYDPG AG
Sbjct 142 TRQQQPGGDVVTTIDPAVQRAAMQGLGSQKGAVAAIEPATGRILALASTPSYDPGAIAGT 201
Query 556 TKDDEKAWTALDSDPNKPMLNRALRETYPPGSTFKLVTAATAFETGKYQSPSDVTDTPDQ 735
D+ W L +DP++PMLNRALR+ YPPGSTFK+VTAA A G T P
Sbjct 202 GTADQDTWKRLQADPDQPMLNRALRQIYPPGSTFKVVTAAAALAAGVVTDIESPTGAPYP 261
Query 736 YILPGTSTPLINASPTEDCGNATVQHAMDLSCNTVFGKMGAELGGDKLRAQAEKFGFNK- 912
Y++PGT+TPL N + + ++ AM LSCN+V G +G + G D++ A A+ FGFN
Sbjct 262 YVMPGTTTPLTNDTSACNQEGLSLDDAMTLSCNSVMGWLGVQTGLDRMTAMAKNFGFNDA 321
Query 913 EVTIPIRADASHFPSGSKPDGTAMDAIGQHDTRATPLQMAMVAAAIANNGSLMEPYLVAQ 1092
++ P+RA S+F + A+ +IGQ+DT ATPL MAMVAA A +G +M P LV +
Sbjct 322 KLDTPVRAARSNFDTTMNASQLALSSIGQYDTAATPLTMAMVAAGAAADGQVMYPQLVDR 381
Query 1093 EGSA-GNVISTHTEHQLSQAVSPATAQKLQQLMESVVQHGTGTNAKIPGVTVGGKTGTAQ 1269
A G+ + QA P AQ+L++LM VVQ+GTG NA+IPG VGGKTGTAQ
Sbjct 382 LTKADGSQVRLMHPVLYRQAFGPGVAQQLRRLMVDVVQNGTGGNARIPGAEVGGKTGTAQ 441
Query 1270 HGQDNSGLPFAWFVSYAK-GQDGKQVAVAVVVEDGSSEAQQISGGSLAAPIAKAMMQAAL 1446
HG DN+G P+AWF+++A+ VAV V S+A I+GG LAAPIAKA+MQ+ L
Sbjct 442 HGVDNTGTPYAWFIAWARPAGSTATPPVAVAVVVADSQASDITGGGLAAPIAKAVMQSVL 501
Query 1447 GK 1452
+
Sbjct 502 DR 503
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Mar 1, 2018 5:11 PM
Number of letters in database: 57,881,132
Number of sequences in database: 174,420
Lambda K H
0.314 0.131 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 174420
Number of Hits to DB: 556788
Number of extensions: 18422
Number of successful extensions: 133
Number of sequences better than 10: 2
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 130
Number of HSP's successfully gapped: 2
Length of query: 1455
Length of database: 57881132
Length adjustment: 113
Effective length of query: 1342
Effective length of database: 38171672
Effective search space: 14199861984
Effective search space used: 14199861984
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 67 (30.4 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
QUESTIONS
- How does this output differ from that of the same search conducted through the web browser interface?
Exercise 01 (10min)¶
Using the sequence in the file data/kitasatospora/lantibiotic.fasta as a query, can you do the following?
- Conduct a remote `BLASTX` query against the `nr` database, restricted to Kitasatospora proteins
- Write the output to a new file called `lantibiotic_blastx_nr_kitasatospora.txt` in the output directory `output/kitasatospora`.
- Compare the results to the output from Exercise 01 in `notebook 03`.
In [9]:
# SOLUTION - EXERCISE 01
# Load sequence of lantibiotic synthesis protein, and inspect the information
seq = SeqIO.read("data/kitasatospora/lantibiotic.fasta", "fasta")
# Run the BLASTX search on the nr database, restricted to Kitasatospora spp.
result_handle = NCBIWWW.qblast("blastx", "nr", seq.format("fasta"),
entrez_query="kitasatospora[ORGN]",
format_type="Text")
# Save output to file
outfilename = os.path.join('output', 'kitasatospora', 'lantibiotic_blastx_nr_kitasatospora.txt')
with open(outfilename, 'w') as outfh:
outfh.write(result_handle.read())